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公开(公告)号：US20110101215A1
公开(公告)日：2011-05-05
申请号：US12921087
申请日：2009-03-27
IPC分类号： H01J49/00
CPC分类号： G01N30/8675 , G01N30/7233 , H01J49/0009
摘要： In quantitation without using the isotope labeling technique, there is no means to detect the presence/absence and the time region of the occurrence of quantitative analysis-inhibitory factors in data for the analysis, and the reliability of the data for the analysis cannot be evaluated. Also, the error of the data due to the occurrence of the quantitative analysis-inhibitory factors cannot be evaluated. In order to solve the problems, first, an internal standard to be detected simultaneously with a component for the analysis is mixed in a mobile phase or an eluate of a liquid chromatograph; under the condition where no quantitative analysis-inhibitory factors occur, a blank sample is analyzed to acquire a mass chromatogram of ions originated from the internal standard; and the result is stored in a data storage unit. Then, a sample for the analysis is mixed to acquire data for the analysis of the sample; and the intensity of ions originated from the internal standard is compared with that of the blank sample in the analysis real time in a data analysis unit. At this time, if an inconsistency exceeding a predetermined threshold is detected, the occurrence of the quantitative analysis-inhibitory factors can be detected. Further, based on the inconsistency, the error range of the data can be given to a data set and the like.
摘要翻译： 在不使用同位素标记技术的定量中，无法检测分析数据中定量分析抑制因子的发生的存在/不存在和时间区域，并且不能评估用于分析的数据的可靠性 。 此外，由于定量分析抑制因子的发生导致的数据误差不能被评估。 为了解决上述问题，首先，将与分析用成分同时检测的内标与液相色谱仪的流动相或洗脱液混合， 在没有定量分析抑制因子发生的条件下，分析空白样品以获得源自内标的离子的质谱图; 结果存储在数据存储单元中。 然后，将用于分析的样本混合以获取用于样品分析的数据; 在数据分析单元中实时分析从内部标准产生的离子强度与空白样本的强度进行比较。 此时，如果检测到超过预定阈值的不一致，则可以检测定量分析抑制因子的发生。 此外，基于不一致性，可以向数据集等提供数据的误差范围。
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公开(公告)号：US20130302821A1
公开(公告)日：2013-11-14
申请号：US13885921
申请日：2011-11-17
申请人： Takeshi Sakamoto , Hiroko Hanzawa , Naomi Manri , Yuji Kuge
发明人： Takeshi Sakamoto , Hiroko Hanzawa , Naomi Manri , Yuji Kuge
IPC分类号： G01N33/68
CPC分类号： G01N33/6893 , A61B5/0071 , A61B5/02007 , A61B5/055 , A61B6/504 , A61B6/508 , A61B8/0891 , G01N2800/323 , G01N2800/52
摘要： The present invention provides a method and a means for evaluating atherosclerotic lesions by identifying a protein (marker protein) group, the expression level of which varies according to the progression of the atherosclerotic lesion, and using the proteins. Specifically, the present invention relates to a method for evaluating atherosclerotic lesions, comprising the steps of detecting a marker protein exhibiting an expression pattern (expression variation) characteristic at a specific disease stage of atherosclerotic lesions in a subject, and evaluating the atherosclerotic lesions in the subject based on the detection result.
摘要翻译： 本发明提供了一种通过鉴定其表达水平根据动脉粥样硬化病变的进展以及使用蛋白质而变化的蛋白质（标记蛋白）组来评价动脉粥样硬化病变的方法和手段。 具体而言，本发明涉及评价动脉粥样硬化病变的方法，其特征在于，包括以下步骤：检测在受试者的动脉粥样硬化病变的特定疾病阶段具有特征的表达模式（表达变异）的标记蛋白，并评价动脉粥样硬化病变 主体根据检测结果。
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公开(公告)号：US09175346B2
公开(公告)日：2015-11-03
申请号：US13643590
申请日：2011-04-19
申请人： Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri , Yuji Kuge
发明人： Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri , Yuji Kuge
CPC分类号： C12Q1/6883 , C12Q2600/112 , C12Q2600/158 , G01N33/18 , G01N33/1826 , G01N33/564 , G01N33/86 , G01N2333/4716 , G01N2800/323 , G01N2800/50 , G01N2800/52
摘要： Arteriosclerosis induces cerebral infarction and myocardial infarction. A multi-marker (a group of protein markers) that assesses the accurate pathogenesis of arteriosclerosis and enables the selection of an adequate treatment method for arteriosclerosis and prediction of the progression of arteriosclerosis, and an evaluation method for the diagnosis, prevention, and treatment of arteriosclerosis that uses said marker group as an indicator have been sought. The present invention relates to A method for evaluation of arteriosclerosis comprising the steps of (a) measuring the expression of von Willebrand factor and/or complement factor D in a sample derived from a subject, (b) measuring the expression of complement component C8 and/or vitamin K-dependent protein Z in the sample derived from the subject, and (c) evaluating arteriosclerosis in the subject on the basis of the results from (a) and (b).
摘要翻译： 动脉硬化引起脑梗死和心肌梗死。 评估动脉硬化的准确发病机理的多标记物（一组蛋白质标记物），能够选择适当的动脉硬化治疗方法和预测动脉硬化的进展，以及诊断，预防和治疗的评价方法 已经寻求使用所述标记物组作为指标的动脉硬化。 本发明涉及一种动脉硬化评估方法，其特征在于，包括以下步骤：（a）测定衍生自受试者的样品中血管性血友病因子和/或补体因子D的表达，（b）测量补体成分C8和 /或来自受试者的样品中的维生素K依赖性蛋白质Z，（c）基于（a）和（b）的结果评价受试者的动脉硬化。
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公开(公告)号：US20130040851A1
公开(公告)日：2013-02-14
申请号：US13643590
申请日：2011-04-19
申请人： Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri , Yuji Kuge
发明人： Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri , Yuji Kuge
IPC分类号： G01N27/62 , C40B30/04 , C12Q1/68 , G01N33/566 , G01N21/64
CPC分类号： C12Q1/6883 , C12Q2600/112 , C12Q2600/158 , G01N33/18 , G01N33/1826 , G01N33/564 , G01N33/86 , G01N2333/4716 , G01N2800/323 , G01N2800/50 , G01N2800/52
摘要： Arteriosclerosis induces cerebral infarction and myocardial infarction. A multi-marker (a group of protein markers) that assesses the accurate pathogenesis of arteriosclerosis and enables the selection of an adequate treatment method for arteriosclerosis and prediction of the progression of arteriosclerosis, and an evaluation method for the diagnosis, prevention, and treatment of arteriosclerosis that uses said marker group as an indicator have been sought. The present invention relates to A method for evaluation of arteriosclerosis comprising the steps of (a) measuring the expression of von Willebrand factor and/or complement factor D in a sample derived from a subject, (b) measuring the expression of complement component C8 and/or vitamin K-dependent protein Z in the sample derived from the subject, and (c) evaluating arteriosclerosis in the subject on the basis of the results from (a) and (b).
摘要翻译： 动脉硬化引起脑梗死和心肌梗死。 评估动脉硬化的准确发病机理的多标记物（一组蛋白质标记物），能够选择适当的动脉硬化治疗方法和预测动脉硬化的进展，以及诊断，预防和治疗的评价方法 已经寻求使用所述标记物组作为指标的动脉硬化。 本发明涉及一种动脉硬化评估方法，其特征在于，包括以下步骤：（a）测定衍生自受试者的样品中血管性血友病因子和/或补体因子D的表达，（b）测量补体成分C8和 /或来自受试者的样品中的维生素K依赖性蛋白质Z，（c）基于（a）和（b）的结果评价受试者的动脉硬化。
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公开(公告)号：US07906759B2
公开(公告)日：2011-03-15
申请号：US12190988
申请日：2008-08-13
申请人： Naomi Manri , Takashi Baba , Hiroyuki Satake
发明人： Naomi Manri , Takashi Baba , Hiroyuki Satake
CPC分类号： H01J49/0045
摘要： An inexpensive mass spectrometer system is provided. This mass spectrometer is capable obtaining structural information of a substance at an improved efficiency, and the time required for the analysis and identification of the substance has been reduced. Identification precision has also been improved. More specifically, this invention provides a tandem mass spectrometer system in which the sample is ionized at the desired polarity, fragment ions obtained by dissociating the ion is analyzed in first or second mass spectrometer section, polarity of the second mass spectrometer is determined based on the result of the analysis, and the mass spectroscopy is carried out. A method for the mass spectroscopy is also provided.
摘要翻译： 提供廉价的质谱仪系统。 该质谱仪能够以提高的效率获得物质的结构信息，并且物质的分析和鉴定所需的时间已经减少。 识别精度也得到了提高。 更具体地，本发明提供一种串联质谱仪系统，其中样品以期望的极性离子化，在第一或第二质谱仪部分分析通过离解离子获得的碎片离子，基于第二质谱仪的极性确定第二质谱仪的极性 分析结果，进行质谱分析。 还提供了用于质谱的方法。
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公开(公告)号：US08603829B2
公开(公告)日：2013-12-10
申请号：US12805619
申请日：2010-08-10
申请人： Yuji Kuge , Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri
发明人： Yuji Kuge , Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri
IPC分类号： G01N33/50
CPC分类号： G01N33/6893 , G01N2800/323 , G01N2800/52
摘要： A method is provided for diagnosing or predicting atherosclerosis with the use of, as indicators, markers (factors) that allow early detection and progression prediction of the disease and a method for evaluating preventive or therapeutic effects. In one form, the present method includes detecting CD166 (ALCAM) level in a sample of a subject such as human. Another aspect of the present method includes evaluating preventive or therapeutic effects of a compound on atherosclerosis. A kit and an apparatus for carrying out such methods is also provided.
摘要翻译： 提供了一种用于诊断或预测动脉粥样硬化的方法，其使用允许疾病的早期检测和进展预测的标志物（因子）作为指标，以及评估预防或治疗效果的方法。 在一种形式中，本方法包括检测人类样本中的CD166（ALCAM）水平。 本方法的另一方面包括评估化合物对动脉粥样硬化的预防或治疗作用。 还提供了用于执行这种方法的套件和装置。
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公开(公告)号：US20110065199A1
公开(公告)日：2011-03-17
申请号：US12805619
申请日：2010-08-10
申请人： Yuji Kuge , Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri
发明人： Yuji Kuge , Hiroko Hanzawa , Takeshi Sakamoto , Naomi Manri
CPC分类号： G01N33/6893 , G01N2800/323 , G01N2800/52
摘要： Since atherosclerosis induces cerebral infarction and myocardial infarction, a method for diagnosing or predicting atherosclerosis with the use of, as indicators, markers (factors) that allow early detection and progression prediction of the disease and a method for evaluating preventive or therapeutic effects have been awaited. This invention provides a method for diagnosing or predicting atherosclerosis which comprises detecting CD166 (ALCAM) level in a sample of a subject such as human, a method for evaluating preventive or therapeutic effects of a compound on atherosclerosis, and a kit and an apparatus for carrying out such methods.
摘要翻译： 由于动脉粥样硬化引起脑梗死和心肌梗死，因此已经等待了使用可以使疾病早期检测和进展预测的标志物（因素）作为诊断或预测动脉粥样硬化的方法，以及评估预防或治疗效果的方法 。 本发明提供一种用于诊断或预测动脉粥样硬化的方法，其包括检测受试者例如人的样品中的CD166（ALCAM）水平，评估化合物对动脉粥样硬化的预防或治疗作用的方法，以及用于携带 出这种方法。
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公开(公告)号：US20090072132A1
公开(公告)日：2009-03-19
申请号：US12190988
申请日：2008-08-13
申请人： Naomi Manri , Takashi Baba , Hiroyuki Satake
发明人： Naomi Manri , Takashi Baba , Hiroyuki Satake
IPC分类号： B01D59/44
CPC分类号： H01J49/0045
摘要： An inexpensive mass spectrometer system is provided. This mass spectrometer is capable obtaining structural information of a substance at an improved efficiency, and the time required for the analysis and identification of the substance has been reduced. Identification precision has also been improved. More specifically, this invention provides a tandem mass spectrometer system in which the sample is ionized at the desired polarity, fragment ions obtained by dissociating the ion is analyzed in first or second mass spectrometer section, polarity of the second mass spectrometer is determined based on the result of the analysis, and the mass spectroscopy is carried out. A method for the mass spectroscopy is also provided.
摘要翻译： 提供廉价的质谱仪系统。 该质谱仪能够以提高的效率获得物质的结构信息，并且物质的分析和鉴定所需的时间已经减少。 识别精度也得到了提高。 更具体地，本发明提供一种串联质谱仪系统，其中样品以期望的极性离子化，在第一或第二质谱仪部分分析通过离解离子获得的碎片离子，基于第二质谱仪的极性确定第二质谱仪的极性 分析结果，进行质谱分析。 还提供了用于质谱的方法。
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