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    Fructosylamine deglycase and a method of producing it
    1.
    发明授权
    Fructosylamine deglycase and a method of producing it 失效

    公开(公告)号：US5387109A

    公开(公告)日：1995-02-07

    申请号：US66499

    申请日：1993-05-24

    申请人： Atsushi Ishikawa ,  Yoshifumi Ohshima ,  Mikio Yamada ,  Hajime Okumura ,  Yoshiya Kawamura

    发明人： Atsushi Ishikawa ,  Yoshifumi Ohshima ,  Mikio Yamada ,  Hajime Okumura ,  Yoshiya Kawamura

    IPC分类号： C12N9/06 ,  C12Q1/26 ,  C12R1/72 ,  C12N9/04 ,  C12N9/08

    CPC分类号： C12N9/0012 ,  C12Q1/26

    摘要： Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an .alpha.-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the .epsilon.-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.

    Biochemical oxygen demand analyzer and methods of analysis using Klebsiella microorganisms
    2.
    发明授权
    Biochemical oxygen demand analyzer and methods of analysis using Klebsiella microorganisms 失效
    生化需氧量分析仪和使用克雷伯菌微生物分析方法

    公开(公告)号：US5356792A

    公开(公告)日：1994-10-18

    申请号：US978843

    申请日：1992-11-19

    申请人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Hatagaki Keizo ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

    发明人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Hatagaki Keizo ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

    IPC分类号： C12Q1/00 ,  G01N33/18 ,  C12Q1/24 ,  G01N27/40

    CPC分类号： G01N33/1806 ,  C12Q1/001 ,  G01N33/1866 ,  G01N33/1893 ,  Y10S435/817 ,  Y10S435/852

    摘要： The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in membrane. Specifically, the present invention relates to a BOD analyzer comprising flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from the group consisting of alginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.

    摘要翻译： 本发明涉及一种包含含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，本发明涉及一种BOD分析装置，其特征在于，包括配置有含有氧电极和微生物膜的微生物传感器的流动池， 以及与流通池的入口连接且配有出口的液体通道。 通过使用至少一种选自藻酸或其盐的凝胶剂，将属于克雷伯氏菌12092菌株的微生物固定在直径为0.65-3μm平均孔径的多孔亲水膜中来制备微生物膜 ，琼脂，结冷胶，黄原胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶和支链淀粉。 本发明还涉及使用BOD分析仪分析BOD的改进方法。

    Process for the production of vinegar with high acetic acid concentration
    4.
    发明授权
    Process for the production of vinegar with high acetic acid concentration 失效
    醋酸浓度高的醋生产工艺

    公开(公告)号：US4364960A

    公开(公告)日：1982-12-21

    申请号：US192468

    申请日：1980-09-30

    申请人： Yoshio Kunimatsu ,  Hajime Okumura ,  Hiroshi Masai ,  Koki Yamada ,  Mikio Yamada

    发明人： Yoshio Kunimatsu ,  Hajime Okumura ,  Hiroshi Masai ,  Koki Yamada ,  Mikio Yamada

    IPC分类号： C12J1/04 ,  C12J1/00

    CPC分类号： C12J1/00

    摘要： A finished vinegar having an acetic acid concentration higher than 20 percent weight by volume is produced by repeating a fermentation cycle wherein a broth is fermented at 27.degree.-32.degree. C. in a 1st submerged fermentation tank by a continuous batch process, and, when the acetic acid concentration of the fermenting broth reaches 12-15 percent weight by volume, the large part of the fermenting broth in the 1st fermentation tank is withdrawn and charged in a 2nd submerged fermentation tank. The 1st fermentation tank is recharged with a mash and the fermentation of the fermenting broth in the 2nd tank is continued under aeration while lowering the temperature of the fermenting broth in such manner that the final fermentation temperature does not fall below 18.degree. C. and the lowering temperature does not become higher than the temperature once lowered.

    摘要翻译： 乙酸浓度高于20体积重量的成品醋通过重复发酵循环来制备，其中在第一浸没发酵罐中通过连续分批过程在27℃-32℃发酵肉汤，并且当 发酵液的乙酸浓度达到12-15重量％，第一发酵罐中大部分发酵液被取出并装入第二个浸没发酵罐中。 第一发酵罐用醪液补充，并且在通气的同时继续在第二罐中发酵发酵液，同时以最终发酵温度不低于18℃的方式降低发酵液的温度，并且 降低温度不会高于一旦降低的温度。

    GOLF BALL
    8.
    发明申请
    GOLF BALL 有权
    高尔夫球

    公开(公告)号：US20100331118A1

    公开(公告)日：2010-12-30

    申请号：US12821770

    申请日：2010-06-23

    申请人： Mutsuhisa FURUKAWA ,  Ryo Mashita ,  Kazuyoshi Shiga ,  Takashi Sasaki ,  Mikio Yamada

    发明人： Mutsuhisa FURUKAWA ,  Ryo Mashita ,  Kazuyoshi Shiga ,  Takashi Sasaki ,  Mikio Yamada

    IPC分类号： A63B37/12

    CPC分类号： A63B37/12 ,  A63B37/0003 ,  A63B37/0024 ,  A63B37/0031 ,  A63B37/0033 ,  A63B37/0062

    摘要： An object of the present invention is to improve the abrasion resistance of a golf ball that uses a polyurethane as a resin component for a cover. Another object of the present invention is to improve the shot feeling of a golf ball that uses a polyurethane as a resin component for a cover. The present invention provides a golf ball comprising a core; and a cover covering the core, wherein the cover contains a polyurethane elastomer as a resin component, and the polyurethane elastomer contains a polyol component and a polyisocyanate component and does not contain a chain extender.

    摘要翻译： 本发明的目的是提高使用聚氨酯作为盖的树脂成分的高尔夫球的耐磨性。 本发明的另一个目的是提高使用聚氨酯作为盖的树脂成分的高尔夫球的射出感。 本发明提供一种包括芯的高尔夫球; 以及覆盖所述芯的盖，其中，所述盖包含作为树脂成分的聚氨酯弹性体，所述聚氨酯弹性体含有多元醇成分和多异氰酸酯成分，不含扩链剂。

    Golf ball
    9.
    发明授权
    Golf ball 失效
    高尔夫球

    公开(公告)号：US5733974A

    公开(公告)日：1998-03-31

    申请号：US516657

    申请日：1995-08-18

    申请人： Mikio Yamada ,  Keiji Moriyama

    发明人： Mikio Yamada ,  Keiji Moriyama

    IPC分类号： A63B37/00 ,  A63B37/12 ,  B29D99/00 ,  B29K21/00 ,  C08L9/00 ,  C08L23/08

    CPC分类号： C08L23/0876 ,  A63B37/0003 ,  A63B37/0033 ,  A63B37/0054 ,  A63B37/0074 ,  A63B37/0075 ,  C08L2205/02 ,  C08L23/0853 ,  C08L9/00

    摘要： The present invention provides a golf ball which exhibits a soft hit feeling and a high impact resilience. The golf ball comprises a core made of an elastomer and a cover covering said core wherein said cover is made of a thermoplastic material comprising a rubber powder and a thermoplastic elastomer, said rubber powder being present in an amount of 5 to 40 parts by weight based on 100 parts by weight of said thermoplastic elastomer.

    摘要翻译： 本发明提供了具有柔软的打击感和高抗冲击性的高尔夫球。 高尔夫球包括由弹性体制成的芯和覆盖所述芯的盖，其中所述盖由包含橡胶粉末和热塑性弹性体的热塑性材料制成，所述橡胶粉末以基于5至40重量份的量存在 在100重量份的所述热塑性弹性体上。