利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

18 条记录 (耗时 0.045 秒)

    Biochemical oxygen demand analyzer and methods of analysis using Klebsiella microorganisms
    1.
    发明授权
    Biochemical oxygen demand analyzer and methods of analysis using Klebsiella microorganisms 失效
    生化需氧量分析仪和使用克雷伯菌微生物分析方法

    公开(公告)号：US5356792A

    公开(公告)日：1994-10-18

    申请号：US978843

    申请日：1992-11-19

    申请人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Hatagaki Keizo ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

    发明人： Shigeru Maeda ,  Akira Ohki ,  Takeshi Sato ,  Naho Kato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Hatagaki Keizo ,  Yasushi Takahashi ,  Mikio Yamada ,  Hajime Okumura

    IPC分类号： C12Q1/00 ,  G01N33/18 ,  C12Q1/24 ,  G01N27/40

    CPC分类号： G01N33/1806 ,  C12Q1/001 ,  G01N33/1866 ,  G01N33/1893 ,  Y10S435/817 ,  Y10S435/852

    摘要： The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in membrane. Specifically, the present invention relates to a BOD analyzer comprising flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from the group consisting of alginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.

    摘要翻译： 本发明涉及一种包含含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，本发明涉及一种BOD分析装置，其特征在于，包括配置有含有氧电极和微生物膜的微生物传感器的流动池， 以及与流通池的入口连接且配有出口的液体通道。 通过使用至少一种选自藻酸或其盐的凝胶剂，将属于克雷伯氏菌12092菌株的微生物固定在直径为0.65-3μm平均孔径的多孔亲水膜中来制备微生物膜 ，琼脂，结冷胶，黄原胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶和支链淀粉。 本发明还涉及使用BOD分析仪分析BOD的改进方法。

    Novel foodstuff from soymilk and method for production thereof
    4.
    发明授权
    Novel foodstuff from soymilk and method for production thereof 失效
    来自豆浆的新型食品及其生产方法

    公开(公告)号：US4996064A

    公开(公告)日：1991-02-26

    申请号：US396662

    申请日：1989-08-22

    申请人： Hirofumi Akano ,  Takeshi Sato ,  Hajime Okumura ,  Yoshiya Kawamura ,  Kyo Shimada

    发明人： Hirofumi Akano ,  Takeshi Sato ,  Hajime Okumura ,  Yoshiya Kawamura ,  Kyo Shimada

    IPC分类号： A23C20/02 ,  A23L11/00

    CPC分类号： A23C20/025 ,  A23L11/09

    摘要： A novel foodstuff is produced by reacting a milk-coagulating enzyme which is produced by a milk-coagulating enzyme-producing microorganism belonging to genus Aspergillus, genus Mucor, or genus Rhizopus and which exhibits a milk-coagulating activity (A) and a protease activity (B) such that the ratio of (A)/(B) is larger than 0.1, with soymilk thereby inducing coagulation, and collecting a resulting coagulated material. The novel foodstuffs produced have the benefit of lacking the bitterness and astringency common to soybean, abounds in emulsifying activity, exhibit a high water-retention property, and are smoothly agreeable to the taste. Thus, they have extensive utility as substitutes for raw materials in conventional processed foods. They are especially suitable for use as raw materials for emulsifiable foods.

    摘要翻译： 通过使属于曲霉属，毛霉属或根霉属的乳凝固酶生成微生物产生的乳化凝固酶与乳固醇活性（A）和蛋白酶活性（A）反应而制造的新型食品 （B）使得（A）/（B）的比例大于0.1，用豆浆从而引起凝结，并收集所得的凝固材料。 所生产的新型食品具有缺乏大豆常见的苦味和涩味的优点，乳化活性大，显示出高保水性，并且味道顺利。 因此，它们作为常规加工食品中原料的替代物具有广泛的用途。 它们特别适合用作可乳化食品的原料。

    Coulometric analysis method and a device therefor
    5.
    发明授权
    Coulometric analysis method and a device therefor 失效
    库仑分析法及其设备

    公开(公告)号：US5909114A

    公开(公告)日：1999-06-01

    申请号：US957393

    申请日：1997-10-23

    申请人： Shunichi Uchiyama ,  Takeshi Sato ,  Hirofumi Akano ,  Yoshiya Kawamura

    发明人： Shunichi Uchiyama ,  Takeshi Sato ,  Hirofumi Akano ,  Yoshiya Kawamura

    IPC分类号： G01N27/42 ,  G01N33/18 ,  G01N27/26

    CPC分类号： G01N27/423 ,  G01N33/1806

    摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance with high reproducibility without any application of voltage etc. from the outside, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.

    摘要翻译： 本发明提供了一种用于定量测定样品物质的快速库仑分析，其重现性高，而没有外部施加电压等，以及用于所述库仑分析的电池和装置。 根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。 本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。

    Sulfur dioxide detector device embodied by using S02-oxidant bioagent
    7.
    发明授权
    Sulfur dioxide detector device embodied by using S02-oxidant bioagent 失效
    通过使用SO2-氧化物生物体实现的硫二氧化物检测器件

    公开(公告)号：US5206170A

    公开(公告)日：1993-04-27

    申请号：US769534

    申请日：1991-10-01

    申请人： Takeshi Sato ,  Nobuko Kubo ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Shigesada Iijima

    发明人： Takeshi Sato ,  Nobuko Kubo ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Shigesada Iijima

    IPC分类号： C12Q1/00 ,  G01N33/00

    CPC分类号： C12Q1/005 ,  C12Q1/001 ,  G01N33/0042 ,  Y10S435/817

    摘要： In a sulfur dioxide detector using SO.sub.2 -oxidant microbes, a bio-membrane is located at one end of an oxygen concentration sensor, the bio-membrane containing the SO.sub.2 -oxidant microbes between an immobilized membrane and a gas permeable membrane. The sensor has an electrode in contact with the immobilized membrane. A dish-shaped or frusto-conical cell has an open end which is in contact with the gas permeable membrane so that SO.sub.2 -laden solution supplied to the cell causes sulfurous acid to oxidize to sulfuric acid as the SO.sub.2 permeates through the bio-membrane so as to change an output from the sensor. An inlet and outlet hole are formed on a sidewall of the cell. The outlet hole is located to be always directly above the inlet hole so as to drain foam formed when the SO.sub.2 -laden solution is supplied to the cell through the inlet hole and drained from the outlet hole.

    Fructosylamine deglycase and a method of producing it
    9.
    发明授权
    Fructosylamine deglycase and a method of producing it 失效

    公开(公告)号：US5387109A

    公开(公告)日：1995-02-07

    申请号：US66499

    申请日：1993-05-24

    申请人： Atsushi Ishikawa ,  Yoshifumi Ohshima ,  Mikio Yamada ,  Hajime Okumura ,  Yoshiya Kawamura

    发明人： Atsushi Ishikawa ,  Yoshifumi Ohshima ,  Mikio Yamada ,  Hajime Okumura ,  Yoshiya Kawamura

    IPC分类号： C12N9/06 ,  C12Q1/26 ,  C12R1/72 ,  C12N9/04 ,  C12N9/08

    CPC分类号： C12N9/0012 ,  C12Q1/26

    摘要： Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an .alpha.-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the .epsilon.-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.

    Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter
    10.
    发明授权
    Electrophoretic medium for electrophoretic separation, gel holder for holding the same medium, slab type electrophoretic apparatus using the same medium and gel holder, and electrophoretic gel cutter 失效
    用于电泳分离的电泳介质，用于保持相同介质的凝胶保持器，使用相同介质和凝胶保持器的板式电泳装置，以及电泳凝胶切割器

    公开(公告)号：US5340461A

    公开(公告)日：1994-08-23

    申请号：US12194

    申请日：1993-02-02

    申请人： Tomoaki Fukushige ,  Hiroko Toda ,  Hirofumi Akano ,  Emiko Ito ,  Tomohiko Fukuta ,  Masahiro Fujimori ,  Yoshiya Kawamura

    发明人： Tomoaki Fukushige ,  Hiroko Toda ,  Hirofumi Akano ,  Emiko Ito ,  Tomohiko Fukuta ,  Masahiro Fujimori ,  Yoshiya Kawamura

    IPC分类号： B01D57/02 ,  G01N27/447 ,  C25B9/00

    CPC分类号： G01N27/44704 ,  B01D57/02 ,  G01N27/44747

    摘要： An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.

    摘要翻译： 最适合作为分离介质的电泳介质由凝胶溶液组成并含有水溶性聚合物，并且可大大提高酶蛋白质的恢复特性。 凝胶保持器设置有止动件，因此可以保持柔软或滑动，同时保持其固定形状，从而允许可预期的物质的可靠分离和纯化，而不会使物质变性。 板式电泳装置允许自动检测样品的电泳状态，并在最佳条件下自动完成电泳，而不设置测量条件，允许连续测量缓冲溶液温度，这对于获得维持酶的电泳条件是重要的 功能，并且允许在非常简单的操作中分离和纯化大量的酶或类似的生理活性物质。 电泳凝胶切割机甚至将大尺寸电泳凝胶切割成精确和安全地变化到所需尺寸，并且特别地在加压状态下切割聚丙烯酰胺等的柔软且容易变形的电泳凝胶，从而允许精确和安全地切割聚丙烯酰胺凝胶，其具有 非常难以切割，不会对凝胶造成伤害。