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    Fluorescent protein
    3.
    发明授权
    Fluorescent protein 有权
    荧光蛋白

    公开(公告)号：US08034614B2

    公开(公告)日：2011-10-11

    申请号：US13008610

    申请日：2011-01-18

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    IPC分类号： C12N5/00 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/43595 ,  G03C1/731 ,  G03C1/733

    摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); (a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

    摘要翻译： 本发明的目的是提供一种新的荧光蛋白，其中可以通过用两种不同波长的光照射来控制其荧光的开和关。 本发明提供下述（a）或（b）所示的荧光蛋白质。 （a）具有SEQ ID NO：3或7所示氨基酸序列的蛋白质; 或（b）相对于SEQ ID NO：3或7所示的氨基酸序列具有氨基酸序列的氨基酸序列的氨基酸序列的氨基酸序列的缺失，取代， 具有光致变色作用。

    Fluorescent protein
    4.
    发明授权
    Fluorescent protein 有权

    公开(公告)号：US07897385B2

    公开(公告)日：2011-03-01

    申请号：US11569275

    申请日：2005-05-20

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    IPC分类号： C12N5/00 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/43595 ,  G03C1/731 ,  G03C1/733

    摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); (a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

    FLUORESCENT PROTEIN
    8.
    发明申请
    FLUORESCENT PROTEIN 有权
    荧光蛋白

    公开(公告)号：US20090155888A1

    公开(公告)日：2009-06-18

    申请号：US11569275

    申请日：2005-05-20

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    IPC分类号： C12N1/21 ,  C07K14/00 ,  C12N15/11 ,  C12N15/00

    CPC分类号： C07K14/43595 ,  G03C1/731 ,  G03C1/733

    摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b);(a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

    摘要翻译： 本发明的目的是提供一种新的荧光蛋白，其中可以通过用两种不同波长的光照射来控制其荧光的开和关。 本发明提供下述（a）或（b）所示的荧光蛋白质。 （a）具有SEQ ID NO：3或7所示氨基酸序列的蛋白质; 或（b）相对于SEQ ID NO：3或7所示的氨基酸序列具有氨基酸序列的氨基酸序列的氨基酸序列的氨基酸序列的缺失，取代， 具有光致变色作用。

    Fluorescent protein
    9.
    发明授权
    Fluorescent protein 失效
    荧光蛋白

    公开(公告)号：US07375201B2

    公开(公告)日：2008-05-20

    申请号：US10504325

    申请日：2003-02-25

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hidetomo Tanase ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hidetomo Tanase ,  Satoshi Karasawa

    IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N5/00

    CPC分类号： C07K14/43595

    摘要： An object of the present invention is to provide a fluorescent protein derived from organisms other than Aequorea victoria and having a novel primary structure. According to the present invention, there is provided a fluorescent protein derived from Halcurias sp. L, which has the following properties: (1) the excitation maximum wavelength is 494 nm, and the fluorescence maximum wavelength is 506 nm; (2) the molar absorption coefficient at 494 nm is 94600 M−1 cm−1; (3) the quantum yield is 0.65; and (4) the pH sensitivity of the fluorescent property is low in the range between pH 4 and pH 11.

    摘要翻译： 本发明的目的是提供一种衍生自维他命A以外的生物体并具有新一级结构的荧光蛋白。 根据本发明，提供了衍生自Halcurias sp。 L具有以下性质：（1）激发最大波长为494nm，荧光最大波长为506nm; （2）在494nm处的摩尔吸光系数为94600M -1 -1 cm -1; （3）量子产率为0.65; 和（4）pH4和pH11之间的荧光特性的pH敏感性低。

    Fluorescent protein
    10.
    发明申请
    Fluorescent protein 失效
    荧光蛋白

    公开(公告)号：US20060127877A1

    公开(公告)日：2006-06-15

    申请号：US10504325

    申请日：2003-02-25

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hidetomo Tanase ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hidetomo Tanase ,  Satoshi Karasawa

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K14/435

    CPC分类号： C07K14/43595

    摘要： An object of the present invention is to provide a fluorescent protein derived from organisms other than Aequorea victoria and having a novel primary structure. According to the present invention, there is provided a fluorescent protein derived from Halcurias sp. L, which has the following properties: (1) the excitation maximum wavelength is 494 nm, and the fluorescence maximum wavelength is 506 nm; (2) the molar absorption coefficient at 494 nm is 94600 M−1cm−1; (3) the quantum yield is 0.65; and (4) the pH sensitivity of the fluorescent property is low in the range between pH 4 and pH 11.

    摘要翻译： 本发明的目的是提供一种衍生自维他命A以外的生物体并具有新一级结构的荧光蛋白。 根据本发明，提供了衍生自Halcurias sp。 L具有以下性质：（1）激发最大波长为494nm，荧光最大波长为506nm; （2）在494nm处的摩尔吸光系数为94600M -1 -1 cm -1; （3）量子产率为0.65; 和（4）pH4和pH11之间的荧光特性的pH敏感性低。