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58 条记录 (耗时 0.028 秒)

    FLUORESCENT PROTEIN
    3.
    发明申请
    FLUORESCENT PROTEIN 有权
    荧光蛋白

    公开(公告)号：US20090155888A1

    公开(公告)日：2009-06-18

    申请号：US11569275

    申请日：2005-05-20

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    IPC分类号： C12N1/21 ,  C07K14/00 ,  C12N15/11 ,  C12N15/00

    CPC分类号： C07K14/43595 ,  G03C1/731 ,  G03C1/733

    摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b);(a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

    摘要翻译： 本发明的目的是提供一种新的荧光蛋白，其中可以通过用两种不同波长的光照射来控制其荧光的开和关。 本发明提供下述（a）或（b）所示的荧光蛋白质。 （a）具有SEQ ID NO：3或7所示氨基酸序列的蛋白质; 或（b）相对于SEQ ID NO：3或7所示的氨基酸序列具有氨基酸序列的氨基酸序列的氨基酸序列的氨基酸序列的缺失，取代， 具有光致变色作用。

    Fluorescent protein
    4.
    发明授权
    Fluorescent protein 有权
    荧光蛋白

    公开(公告)号：US08034614B2

    公开(公告)日：2011-10-11

    申请号：US13008610

    申请日：2011-01-18

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    IPC分类号： C12N5/00 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/43595 ,  G03C1/731 ,  G03C1/733

    摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); (a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

    摘要翻译： 本发明的目的是提供一种新的荧光蛋白，其中可以通过用两种不同波长的光照射来控制其荧光的开和关。 本发明提供下述（a）或（b）所示的荧光蛋白质。 （a）具有SEQ ID NO：3或7所示氨基酸序列的蛋白质; 或（b）相对于SEQ ID NO：3或7所示的氨基酸序列具有氨基酸序列的氨基酸序列的氨基酸序列的氨基酸序列的缺失，取代， 具有光致变色作用。

    Fluorescent protein
    5.
    发明授权
    Fluorescent protein 有权

    公开(公告)号：US07897385B2

    公开(公告)日：2011-03-01

    申请号：US11569275

    申请日：2005-05-20

    申请人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    发明人： Atsushi Miyawaki ,  Ryoko Ando ,  Hideaki Mizuno ,  Satoshi Karasawa

    IPC分类号： C12N5/00 ,  C07K14/00 ,  C07H21/04

    CPC分类号： C07K14/43595 ,  G03C1/731 ,  G03C1/733

    摘要： The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); (a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.

    Vehicle and method of mounting gas fuel tank
    9.
    发明授权
    Vehicle and method of mounting gas fuel tank 有权
    车辆和安装气体燃料箱的方法

    公开(公告)号：US09260009B2

    公开(公告)日：2016-02-16

    申请号：US11794095

    申请日：2006-01-06

    申请人： Hideaki Mizuno ,  Yoshiaki Naganuma

    发明人： Hideaki Mizuno ,  Yoshiaki Naganuma

    IPC分类号： B60K11/04 ,  B60K15/035 ,  B60K15/063 ,  B60K15/067 ,  B60H1/32 ,  B60H1/24 ,  B60H1/26 ,  B65D51/16 ,  B65D88/12 ,  B60P3/00 ,  B60K15/07

    CPC分类号： B60K15/07 ,  B60K2015/0639 ,  B60Y2200/143 ,  B60Y2200/1432

    摘要： A hydrogen tank is mounted on a roof so that a valve unit mounted to one end in a longitudinal direction of the hydrogen tank is separated as far apart as possible from a front platform and a middle platform of a fuel cell equipped bus and so that the longitudinal direction of the hydrogen tank is in a lateral direction of a vehicle. Thus, even if hydrogen leaks from the valve unit or a fusible plug valve of the valve unit is fused to discharge hydrogen from the tank, the influence of the leaking or discharged hydrogen on the front platform and the middle platform can be reduced.

    摘要翻译： 氢罐安装在屋顶上，使得安装在氢罐纵向方向上的一端的阀单元尽可能远离燃料电池配备的总线的前平台和中间平台分开， 氢罐的纵向方向是在车辆的横向方向上。 因此，即使氢气从阀单元泄漏，也可以将阀单元的易熔塞阀熔融，从罐中排出氢气，可以减少前台和中间平台上泄漏或排出的氢气的影响。

    BONDING METHOD AND MEMBERS TO BE BONDED
    10.
    发明申请
    BONDING METHOD AND MEMBERS TO BE BONDED 审中-公开
    绑定方式和会员绑定

    公开(公告)号：US20130255619A1

    公开(公告)日：2013-10-03

    申请号：US13994217

    申请日：2011-12-09

    申请人： Hideaki Mizuno ,  Toru Fukami ,  Kenshi Ushijima

    发明人： Hideaki Mizuno ,  Toru Fukami ,  Kenshi Ushijima

    IPC分类号： B23K11/00 ,  F02F1/24

    CPC分类号： B23K11/002 ,  B23K11/093 ,  B23K20/10 ,  B23K28/02 ,  F02F1/24 ,  F02F2200/00

    摘要： A bonding method includes bonding a pair of members together made of conductive material and having bonding surfaces which are not positioned on the same plane. In the bonding method, the bonding surfaces of the members to be bonded are positioned to face each other, an electric current is supplied from one of the members to be bonded to the other for carrying out resistance heating while the pair of members to be bonded are slid relative to each other. In this way, the bonding surfaces are bonded to each other. Split or partition surfaces that form a hollow passage and extending along the axis of the hollow passage are set as the bonding surfaces which are not on the same plane.

    摘要翻译： 接合方法包括将由导电材料制成的一对部件接合在一起并具有不位于同一平面上的接合面。 在接合方法中，要结合的部件的接合表面彼此面对，从要接合的部件之一供给电流以进行电阻加热，同时将要被接合的一对部件 相对于彼此滑动。 以这种方式，接合表面彼此结合。 形成中空通道并沿着中空通道的轴线延伸的分隔或分隔面被设定为不在同一平面上的接合表面。