公开(公告)号：US20190032039A1

公开(公告)日：2019-01-31

申请号：US16157538

申请日：2018-10-11

Applicant: BASF Enzymes LLC ,  Syngenta Participations AG

Inventor： Malgorzata Slupska ,  Geoff Hazlewood ,  Cathy Chang ,  Peter Luginbuhl ,  Ellen Burke ,  Michelle Cayouette ,  Uvini Gunawardena ,  Majid Ghassemian ,  Aron Silverstone ,  Yan Zhang

IPC: C12N9/96 ,  C12N9/26 ,  C12N9/34

Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

公开(公告)号：US09150845B2

公开(公告)日：2015-10-06

申请号：US14014567

申请日：2013-08-30

Applicant: BASF ENZYMES LLC

Inventor： David P. Weiner ,  Alexander Varvak ,  Toby Richardson ,  Mircea Podar ,  Ellen Burke ,  Shaun Healey

IPC: C12P7/42 ,  C12N9/88

CPC classification number: C12N9/88 ,  C12P7/42 ,  C12P13/222 ,  C12P13/225 ,  C12P13/24 ,  C12Y403/01005 ,  C12Y403/01023 ,  D06M16/003

Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

Abstract translation: 本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸 和多肽。 本发明的多肽可用于多种药物，农业和工业环境。

公开(公告)号：US10100299B2

公开(公告)日：2018-10-16

申请号：US13674199

申请日：2012-11-12

Applicant: BASF Enzymes LLC ,  Syngenta Participation AG

Inventor： Malgorzata Slupska ,  Geoff Hazlewood ,  Cathy Chang ,  Peter Luginbuhl ,  Ellen Burke ,  Michelle Cayouette ,  Uvini Gunawardena ,  Majid Ghassemian ,  Aron Silverstone ,  Yan Zhang

IPC: C12P7/10 ,  C12P7/06 ,  C12N9/34 ,  C12N9/26 ,  C12N9/24 ,  C12N9/14 ,  C12N9/10 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C07H21/04 ,  C07K1/00 ,  C12N9/96

Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

公开(公告)号：US09719079B2

公开(公告)日：2017-08-01

申请号：US15009414

申请日：2016-01-28

Applicant: BASF Enzymes LLC

Inventor： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

IPC: C12N9/10 ,  C12Q1/68 ,  C12P21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/88 ,  C12N15/82 ,  C12P17/10 ,  A61K38/00

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

公开(公告)号：US11739312B2

公开(公告)日：2023-08-29

申请号：US17110222

申请日：2020-12-02

Applicant: BASF Enzymes LLC ,  Syngenta Participations AG

Inventor： Malgorzata Slupska ,  Geoff Hazlewood ,  Cathy Chang ,  Peter Luginbuhl ,  Ellen Burke ,  Michelle Cayouette ,  Uvini Gunawardena ,  Majid Ghassemian ,  Aron Silverstone ,  Yan Zhang

IPC: C12N9/96 ,  C12N9/26 ,  C12N9/34

CPC classification number: C12N9/96 ,  C12N9/2408 ,  C12N9/2414 ,  C12N9/2425 ,  C12N9/2428 ,  Y02E50/10

Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

公开(公告)号：US10883098B2

公开(公告)日：2021-01-05

申请号：US16157538

申请日：2018-10-11

Applicant: BASF Enzymes LLC ,  Syngenta Participations AG

Inventor： Malgorzata Slupska ,  Geoff Hazlewood ,  Cathy Chang ,  Peter Luginbuhl ,  Ellen Burke ,  Michelle Cayouette ,  Uvini Gunawardena ,  Majid Ghassemian ,  Aron Silverstone ,  Yan Zhang

IPC: C12N9/96 ,  C12N9/26 ,  C12N9/34

Abstract: In one aspect, the invention is directed to polypeptides having an amylase and/or glucoamylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of polysaccharide, oligosaccharide or starch into sugars. In one aspect, the invention provides delayed release compositions comprising an desired ingredient coated by a latex polymer coating. In alternative embodiments, enzymes are used to make biofuels, e.g., ethanol, butanol, propanol, or a gasoline-ethanol mix, including a bioethanol, biopropanol, biobutanol, or a biodiesel, or for any form of fuel or biomass processing.

公开(公告)号：US09290755B2

公开(公告)日：2016-03-22

申请号：US13966880

申请日：2013-08-14

Applicant: BASF ENZYMES LLC

Inventor： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

IPC: C12N9/10 ,  C12Q1/68 ,  C12P21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/88 ,  C12N15/82 ,  C12P17/10 ,  A61K38/00

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.