公开(公告)号：US09290755B2

公开(公告)日：2016-03-22

申请号：US13966880

申请日：2013-08-14

Applicant: BASF ENZYMES LLC

Inventor： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

IPC: C12N9/10 ,  C12Q1/68 ,  C12P21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/88 ,  C12N15/82 ,  C12P17/10 ,  A61K38/00

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

公开(公告)号：US09169478B2

公开(公告)日：2015-10-27

申请号：US13967157

申请日：2013-08-14

Applicant: BASF Enzymes LLC

Inventor： David P. Weiner ,  Ellen G. Burke ,  Peter Luginbuhl ,  Analia Bueno ,  Joslin M. Cuenca ,  Mervyn L. De Souza ,  Sherry Kollmann

IPC: C12N9/90 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/20 ,  C12N1/00 ,  C12N5/00 ,  C12N5/07 ,  C12N5/04 ,  C07H21/04 ,  C07K1/00 ,  A01K67/027 ,  C12Q1/533 ,  A23G4/12 ,  A23K1/165 ,  A23L1/03 ,  A23G3/36 ,  A23G4/14 ,  A61K38/00

CPC classification number: C12N9/90 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/10 ,  A23G3/366 ,  A23G4/123 ,  A23G4/14 ,  A23K20/189 ,  A23L29/06 ,  A23V2002/00 ,  A61K38/00 ,  A61K38/52 ,  C12Q1/533 ,  C12Y501/00 ,  C12Y501/01

Abstract: This invention relates generally to enzymes, polynucleotides encoding the enzymes having isomerase activity, e.g., racemase activity, e.g., amino acid racemase activity, alanine racemase activity, and/or epimerase activity, and/or catalyze the re-arrangement of atoms within a molecule, catalyze the conversion of one isomer into another, catalyze the conversion of an optically active substrate into a raceme, which is optically inactive, catalyze the interconversion of substrate enantiomers, catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry, catalyze the stereochemical inversion of the configuration around an asymmetric carbon atom in a substrate having more than one asymmetric center, and/or catalyze the racemization of amino acids.

Abstract translation: 本发明一般涉及酶，编码具有异构酶活性的酶的多核苷酸，例如消旋酶活性，例如氨基酸消旋酶活性，丙氨酸消旋酶活性和/或差向异构酶活性，和/或催化分子内原子重新排列 催化一种异构体转化为另一种异构体，催化光学活性底物转化为光学无活性的外消旋催化底物对映异构体的相互转化，催化仅具有一个中心的底物中不对称碳原子周围的立体化学反应 的不对称性，催化具有多于一个不对称中心的底物中不对称碳原子周围构型的立体化学反转，和/或催化氨基酸的外消旋化。

公开(公告)号：US09150845B2

公开(公告)日：2015-10-06

申请号：US14014567

申请日：2013-08-30

Applicant: BASF ENZYMES LLC

Inventor： David P. Weiner ,  Alexander Varvak ,  Toby Richardson ,  Mircea Podar ,  Ellen Burke ,  Shaun Healey

IPC: C12P7/42 ,  C12N9/88

CPC classification number: C12N9/88 ,  C12P7/42 ,  C12P13/222 ,  C12P13/225 ,  C12P13/24 ,  C12Y403/01005 ,  C12Y403/01023 ,  D06M16/003

Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

Abstract translation: 本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸 和多肽。 本发明的多肽可用于多种药物，农业和工业环境。

公开(公告)号：US09719079B2

公开(公告)日：2017-08-01

申请号：US15009414

申请日：2016-01-28

Applicant: BASF Enzymes LLC

Inventor： David P. Weiner ,  Lishan Zhao ,  Peter Luginbuhl ,  Ellen Burke ,  Toby H. Richardson ,  Paula M. Hicks

IPC: C12N9/10 ,  C12Q1/68 ,  C12P21/04 ,  C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/04 ,  C12N9/88 ,  C12N15/82 ,  C12P17/10 ,  A61K38/00

CPC classification number: C12N9/88 ,  A01K2217/05 ,  A61K38/00 ,  C07K2317/92 ,  C12N9/1022 ,  C12N15/8243 ,  C12P17/10 ,  C12Y401/03016 ,  C12Y401/03017 ,  Y02E50/16 ,  Y02E50/17

Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.