公开(公告)号：US10546735B2

公开(公告)日：2020-01-28

申请号：US14361885

申请日：2012-11-30

Applicant: bioMerieux, Inc.

Inventor： Grégory Strubel ,  Maud Arsac ,  Denis Desseree ,  Pierre-Jean Cotte-Pattat ,  Pierre Mahe

IPC: H01J49/00 ,  G01N33/483 ,  H01J49/26

Abstract: An identification by mass spectrometry of a microorganism from among reference microorganisms represented by reference data sets includes: determining a set of data of the microorganism according to a spectrum; for each reference microorganism, calculating a distance between the determined and reference sets; and calculating a probability f(m) according to relation f ⁡ ( m ) = pN ⁡ ( m ❘ μ , σ ) pN ⁡ ( m ❘ μ , σ ) + ( 1 - p ) ⁢ N ⁡ ( m ❘ μ _ , σ _ ) where: m is the distance calculated for the reference microorganism; N(m|μ,σ) is the value, for m, of a random variable modeling the distance between a reference microorganism to be identified and the reference microorganism, when the microorganism is the reference microorganism; N(m|μ,σ) is the value, for m, of a random variable modeling the distance between a microorganism to be identified and the reference microorganism, when the microorganism is not the reference microorganism; and p is a scalar in the range from 0 to 1.

公开(公告)号：US11085091B2

公开(公告)日：2021-08-10

申请号：US16353386

申请日：2019-03-14

Applicant: BIOMERIEUX

Inventor： Maud Arsac ,  Bertrand Bonnaud ,  Francois Mallet ,  Jean-Philippe Pichon

IPC: C12Q1/70 ,  C12Q1/6886

Abstract: The invention relates to a method for in vitro diagnosis or prognosis of testicular cancer which comprises a step of detecting the presence or absence of at least one expression product from at least one nucleic acid sequence selected from the sequences identified in SEQ ID NOS: 1 to 6 or from the sequences which exhibit at least 99% identity with one of the sequences identified in SEQ ID NOS: 1 to 6, to isolated nucleic acid sequences and to the use thereof as a testicular cancer marker.

公开(公告)号：US11562165B2

公开(公告)日：2023-01-24

申请号：US15569005

申请日：2016-04-21

Applicant: BIOMERIEUX

Inventor： Maud Arsac ,  Pierre-Jean Cotte-Pattat ,  Victoria Girard ,  Valérie Monnin

IPC: G06K9/00 ,  G16B40/10 ,  G16B40/20 ,  C12Q1/04 ,  G06F16/28 ,  G06K9/62 ,  H01J49/00 ,  H01J49/16 ,  H01J49/40

Abstract: A method for identifying by mass spectrometry an unknown microorganism subgroup among a set of reference subgroups, including a step of constructing one knowledgebase and one classifying model per associated subgroup on the basis of the acquisition of at least one set of learning spectra of microorganisms identified as belonging to the subgroups of a group and including: constructing an adjusting model allowing mass-to-charge offsets of the acquired spectra to be corrected on the basis of reference masses-to-charges that are common to the various subgroups; adjusting the masses-to-charges of all of the lists of peaks of the learning spectra and constructing one classifying model per subgroup and the associated knowledgebase on the basis of the adjusted learning spectra.

公开(公告)号：US12191129B2

公开(公告)日：2025-01-07

申请号：US16708419

申请日：2019-12-09

Applicant: BIOMERIEUX, INC.

Inventor： Grégory Strubel ,  Maud Arsac ,  Denis Desseree ,  Pierre-Jean Cotte-Pattat

IPC: H01J49/00 ,  G01N33/68 ,  G16B40/10 ,  G16B99/00

Abstract: A method of identifying a microorganism by mass spectrometry, including acquiring at least one mass spectrum of said microorganism; for each acquired mass spectrum: detecting peaks of the spectrum in a predetermined mass range; generating a list of peaks identifying at most one peak in each interval of a predetermined subdivision of the range of mass-to-charge ratios, the width of the intervals of the subdivision logarithmically increasing along with the mass-to-charge ratio, and analyzing the list(s) of peaks obtained according to a knowledge base of previously-identified microorganisms and/or types of microorganisms.

公开(公告)号：US10541119B2

公开(公告)日：2020-01-21

申请号：US14361794

申请日：2012-11-30

Applicant: Biomerieux, Inc.

Inventor： Grégory Strubel ,  Maud Arsac ,  Denis Desseree ,  Pierre-Jean Cotte-Pattat

IPC: H01J49/00 ,  G16B99/00

Abstract: A method of identifying a microorganism by mass spectrometry, including acquiring at least one mass spectrum of said microorganism; for each acquired mass spectrum: detecting peaks of the spectrum in a predetermined mass range; generating a list of peaks identifying at most one peak in each interval of a predetermined subdivision of the range of mass-to-charge ratios, the width of the intervals of the subdivision logarithmically increasing along with the mass-to-charge ratio, and analyzing the list(s) of peaks obtained according to a knowledge base of previously-identified microorganisms and/or types of microorganisms.

公开(公告)号：US20190203306A1

公开(公告)日：2019-07-04

申请号：US16353386

申请日：2019-03-14

Applicant: BIOMERIEUX

Inventor： Maud Arsac ,  Bertrand Bonnaud ,  Francois Mallet ,  Jean-Philippe Pichon

IPC: C12Q1/70 ,  C12Q1/6886

CPC classification number: C12Q1/702 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/158

Abstract: The invention relates to a method for in vitro diagnosis or prognosis of testicular cancer which comprises a step of detecting the presence or absence of at least one expression product from at least one nucleic acid sequence selected from the sequences identified in SEQ ID NOS: 1 to 6 or from the sequences which exhibit at least 99% identity with one of the sequences identified in SEQ ID NOS: 1 to 6, to isolated nucleic acid sequences and to the use thereof as a testicular cancer marker.

公开(公告)号：US20140343864A1

公开(公告)日：2014-11-20

申请号：US14361885

申请日：2012-11-30

Applicant: bioMerieux, Inc.

Inventor： Grégory Strubel ,  Maud Arsac ,  Denis Desseree ,  Pierre-Jean Cotte-Pattat ,  Pierre Mahe

IPC: H01J49/00 ,  G01N33/483

CPC classification number: H01J49/0036 ,  C12Q1/04 ,  G01N33/483 ,  G01N33/6848 ,  G06K9/00147 ,  G06K9/6269 ,  G06K9/627 ,  G06K9/6278 ,  H01J49/26

Abstract: An identification by mass spectrometry of a microorganism from among reference microorganisms represented by reference data sets includes: determining a set of data of the microorganism according to a spectrum; for each reference microorganism, calculating a distance between the determined and reference sets; and calculating a probability f(m) according to relation f  ( m ) = pN  ( m  μ , σ ) pN  ( m  μ , σ ) + ( 1 - p )  N  ( m  μ _ , σ _ ) where: m is the distance calculated for the reference microorganism; N(m|μ,σ) is the value, for m, of a random variable modeling the distance between a reference microorganism to be identified and the reference microorganism, when the microorganism is the reference microorganism; N(m| μ, σ) is the value, for m, of a random variable modeling the distance between a microorganism to be identified and the reference microorganism, when the microorganism is not the reference microorganism; and p is a scalar in the range from 0 to 1.

Abstract translation: 通过参考数据组所表示的参考微生物中的微生物的质谱鉴定包括：根据光谱确定微生物的一组数据; 对于每个参考微生物，计算所确定的和参考组之间的距离; 并且根据关系式（m）= pN（mμ，＆sgr;）pN（mμ，＆sgr）+（1-p）N（mμ）计算概率f（m） _，＆sgr; _）其中：m是参考微生物计算的距离; 当微生物是参照微生物时，N（m |μ，＆sgr;）是m的值，用于建立待鉴定的参考微生物与参照微生物之间的距离的随机变量; N（m |μ，＆sgr;）是当微生物不是参照微生物时，随机变量建模待鉴定微生物与参照微生物之间的距离的m值; p是0到1范围内的标量。

公开(公告)号：US12091702B2

公开(公告)日：2024-09-17

申请号：US16708417

申请日：2019-12-09

Applicant: BIOMERIEUX, INC.

Inventor： Grégory Strubel ,  Maud Arsac ,  Denis Desseree ,  Pierre-Jean Cotte-Pattat ,  Pierre Mahe

IPC: C12Q1/04 ,  C12Q1/6872 ,  G01N33/483 ,  G01N33/68 ,  G06F18/2411 ,  G06F18/2413 ,  G06F18/2415 ,  G06V20/69 ,  G16B40/00 ,  G16B40/10 ,  G16B40/20 ,  H01J49/00 ,  H01J49/26

CPC classification number: C12Q1/04 ,  C12Q1/6872 ,  G01N33/483 ,  G01N33/6848 ,  G06F18/2411 ,  G06F18/2413 ,  G06F18/24155 ,  G06V20/698 ,  G16B40/00 ,  G16B40/10 ,  G16B40/20 ,  H01J49/0036 ,  H01J49/26

Abstract: An identification by mass spectrometry of a microorganism from among reference microorganisms represented by reference data sets includes: determining a set of data of the microorganism according to a spectrum; for each reference microorganism, calculating a distance between the determined and reference sets; and calculating a probability ƒ(m) according to relation





f
⁡

(
m
)


=


pN
⁡

(


m
|
μ

,
σ

)




pN
⁡

(


m
|
μ

,
σ

)


+


(

1
-
p

)

⁢

N
⁡

(


m
|

μ
_


,

σ
_


)









where: m is the distance calculated for the reference microorganism; N(m|μ,σ) is the value, for m, of a random variable modeling the distance between a reference microorganism to be identified and the reference microorganism, when the microorganism is the reference microorganism; N(m|μ,σ) is the value, for m, of a random variable modeling the distance between a microorganism to be identified and the reference microorganism, when the microorganism is not the reference microorganism; and p is a scalar in the range from 0 to 1.

公开(公告)号：US10240213B2

公开(公告)日：2019-03-26

申请号：US13739447

申请日：2013-01-11

Applicant: BIOMERIEUX

Inventor： Maud Arsac ,  Bertrand Bonnaud ,  Francois Mallet ,  Jean-Philippe Pichon

IPC: C12Q1/6886 ,  C12Q1/70

Abstract: The invention relates to a method for in vitro diagnosis or prognosis of testicular cancer which comprises a step of detecting the presence or absence of at least one expression product from at least one nucleic acid sequence selected from the sequences identified in SEQ ID NOS: 1 to 6 or from the sequences which exhibit at least 99% identity with one of the sequences identified in SEQ ID NOS: 1 to 6, to isolated nucleic acid sequences and to the use thereof as a testicular cancer marker.

公开(公告)号：US20140358449A1

公开(公告)日：2014-12-04

申请号：US14361794

申请日：2012-11-30

Applicant: BIOMERIEUX, INC.

Inventor： Grégory Strubel ,  Maud Arsac ,  Denis Desseree ,  Pierre-Jean Cotte-Pattat

IPC: H01J49/00 ,  G06F19/10

Abstract: A method of identifying a microorganism by mass spectrometry, including acquiring at least one mass spectrum of said microorganism; for each acquired mass spectrum: detecting peaks of the spectrum in a predetermined mass range; generating a list of peaks identifying at most one peak in each interval of a predetermined subdivision of the range of mass-to-charge ratios, the width of the intervals of the subdivision logarithmically increasing along with the mass-to-charge ratio, and analyzing the list(s) of peaks obtained according to a knowledge base of previously-identified microorganisms and/or types of microorganisms.

Abstract translation: 一种通过质谱鉴定微生物的方法，包括获取所述微生物的至少一个质谱; 对于每个获得的质谱：检测在预定质量范围内的光谱的峰值; 产生在质量 - 电荷比范围的预定细分的每个间隔中至多标识一个峰的峰列表，细分间隔的宽度与质荷比对数增加，并分析 根据先前确定的微生物和/或微生物类型的知识库获得的峰的列表。