公开(公告)号：US20120264173A1

公开(公告)日：2012-10-18

申请号：US13539555

申请日：2012-07-02

Applicant: BRUCE A. DINER ,  Janine Fan

Inventor： BRUCE A. DINER ,  Janine Fan

IPC: C12P19/00 ,  C12P13/04 ,  C12P7/02

CPC classification number: D21C3/003 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/02 ,  D21C3/006 ,  D21C3/20 ,  Y02E50/16 ,  Y02E50/17

Abstract: Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of one or more alkylamine and optionally one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.

Abstract translation: 在碱性条件下，在一种或多种烷基胺和任选的一种或多种另外的亲核试剂存在下，使用有机溶剂溶液对生物质进行预处理以分离和提取木质素。 预处理的生物质被糖化酶联合体进一步水解。 通过糖化释放的发酵糖可用于通过发酵生产目标化学品。

公开(公告)号：US20120270269A1

公开(公告)日：2012-10-25

申请号：US13539696

申请日：2012-07-02

Applicant: BRUCE A. DINER ,  Janine Fan

Inventor： BRUCE A. DINER ,  Janine Fan

IPC: C12P19/00 ,  C12P7/10 ,  C12P7/16 ,  C12P13/04 ,  C12P7/04

CPC classification number: C12P19/14 ,  C08H8/00 ,  C10G2300/1011 ,  C10G2300/1014 ,  C10G2300/44 ,  C10G2300/805 ,  C10L1/02 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02 ,  D21C3/024 ,  D21C3/20 ,  D21C11/0007 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343 ,  Y02P30/20

Abstract: Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of ammonia and optionally an additional nucleophile to fragment and extract lignin without loss of hemicellulose. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.

Abstract translation: 在碱性条件下，在氨和任选另外的亲核试剂存在下，使用有机溶剂溶液对生物质进行预处理，将木质素分解并提取而不损失半纤维素。 预处理的生物质被糖化酶联合体进一步水解。 通过糖化释放的发酵糖可用于通过发酵生产目标化学品。

公开(公告)号：US20100159519A1

公开(公告)日：2010-06-24

申请号：US12639050

申请日：2009-12-16

Applicant: BRUCE A. DINER ,  Paul Joseph Fagan ,  Janine Fan

Inventor： BRUCE A. DINER ,  Paul Joseph Fagan ,  Janine Fan

IPC: C12P7/02 ,  C07H1/08 ,  C12P19/00 ,  C12P7/40

CPC classification number: C12P7/10 ,  C08H8/00 ,  C10G2300/1014 ,  C10G2300/202 ,  C10G2300/44 ,  C10G2300/805 ,  C10L1/02 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  D21C3/02 ,  D21C3/20 ,  D21C5/00 ,  D21C11/0007 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P30/20

Abstract: Biomass is pretreated using an organic solvent solution under alkaline conditions in the presence of elemental sulfur and optionally one or more alkylamine and/or one or more additional nucleophile to fragment and extract lignin. Pretreated biomass is further hydrolyzed with a saccharification enzyme consortium. Fermentable sugars released by saccharification may be utilized for the production of target chemicals by fermentation.

Abstract translation: 在碱性条件下，在元素硫和任选的一种或多种烷基胺和/或一种或多种另外的亲核试剂的存在下，使用有机溶剂溶液预处理生物质，以片段和提取木质素。 预处理的生物质被糖化酶联合体进一步水解。 通过糖化释放的发酵糖可用于通过发酵生产目标化学品。

公开(公告)号：US20120187228A1

公开(公告)日：2012-07-26

申请号：US13185572

申请日：2011-07-19

Applicant: CARL E. CAMP ,  JELENA CIRAKOVIC ,  BRUCE A. DINER ,  Janine Fan

Inventor： CARL E. CAMP ,  JELENA CIRAKOVIC ,  BRUCE A. DINER ,  Janine Fan

IPC: B02C25/00

CPC classification number: C12P19/00 ,  C08H8/00 ,  C12P7/10 ,  C12P2201/00 ,  D21C3/02 ,  D21C5/00 ,  Y02E50/16 ,  Y02E50/17

Abstract: Mechanical milling of biomass as a pretreatment to render the biomass readily saccharifiable requires high energy input. Preceding mechanical milling by treatment with anhydrous ammonia was found to greatly reduce the energy requirement for fine milling, providing a more economical pretreatment process for commercial use.

Abstract translation: 机械研磨生物质作为预处理以使生物质易于糖化需要高能量输入。 发现用无水氨处理前机械研磨大大降低了精细研磨的能量需求，为商业用途提供了更经济的预处理工艺。

公开(公告)号：US20140094630A1

公开(公告)日：2014-04-03

申请号：US14032328

申请日：2013-09-20

Applicant: DOUGLAS ROBERT ANTON ,  BRUCE A. DINER ,  STEVEN D. DOIG

Inventor： DOUGLAS ROBERT ANTON ,  BRUCE A. DINER ,  STEVEN D. DOIG

IPC: C07C29/86

CPC classification number: C07C29/86 ,  C12P7/04 ,  C12P7/16 ,  C12P21/06 ,  Y02E50/10

Abstract: Provided herein are processes comprising providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; recovering at least a portion of the butanol and extractant composition from the fermentation medium; recycling the extractant composition recovered in (c) one or more times in the fermentation medium; optionally adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition, whereby the rate of oxidation or effect of oxidized products in the recycled extractant composition is substantially reduced and/or avoided such that the extractant composition may be recycled.

Abstract translation: 本文提供的方法包括提供包含可发酵碳源的发酵培养基，包含工程丁醇生物合成途径的重组微生物和丁醇; 使所述发酵培养基与包含有效量的抗氧化剂或抗氧化剂样化合物的提取剂组合物接触，由此发酵培养基中的至少一部分丁醇分配到提取剂中; 从发酵培养基中回收至少一部分丁醇和提取剂组合物; 在（c）中回收的萃取剂组合物在发酵培养基中回收一次或多次; 任选地调节萃取剂组合物中抗氧化剂或抗氧化剂样化合物的有效量，从而大大降低和/或避免了再循环的萃取剂组合物中的氧化产物的氧化速率或氧化产物的影响，从而可以回收萃取剂组合物。

公开(公告)号：US20080274035A1

公开(公告)日：2008-11-06

申请号：US12169063

申请日：2008-07-08

Applicant: BRUCE A. DINER ,  Ming Zheng

Inventor： BRUCE A. DINER ,  Ming Zheng

IPC: C01B31/02

CPC classification number: D01F11/10 ,  B82Y10/00 ,  D01F11/12 ,  D01F11/14 ,  D01F11/16 ,  H01G9/2031 ,  H01L51/0048 ,  H01L51/0052 ,  H01L51/4226 ,  H01M8/20 ,  H01M14/005 ,  Y02E10/542 ,  Y02E10/549 ,  Y02E60/528 ,  Y10S977/704 ,  Y10S977/742 ,  Y10S977/745 ,  Y10S977/746 ,  Y10S977/842 ,  Y10S977/847 ,  Y10S977/895 ,  Y10S977/902 ,  Y10S977/948

Abstract: Carbon nanotubes have been reversibly and readily oxidized and reduced with common chemicals in solution, thereby allowing the nanotubes to be used as catalysts for chemical reactions and as stable charge storage devices.

Abstract translation: 碳纳米管已被可逆地容易地用溶液中的常见化学物质氧化和还原，从而允许纳米管用作化学反应的催化剂和稳定的电荷存储装置。

公开(公告)号：US20110312044A1

公开(公告)日：2011-12-22

申请号：US13162828

申请日：2011-06-17

Applicant: DOUGLAS ROBERT ANTON ,  JELENA CIRAKOVIC ,  BRUCE A. DINER ,  MICHAEL CHARLES GRADY ,  FRANCIS J. WOERNER

Inventor： DOUGLAS ROBERT ANTON ,  JELENA CIRAKOVIC ,  BRUCE A. DINER ,  MICHAEL CHARLES GRADY ,  FRANCIS J. WOERNER

IPC: C12P7/04 ,  C12N1/19 ,  C12N1/15 ,  C12P13/02 ,  C12P7/64 ,  C07C29/04 ,  C12P7/06 ,  C11C3/00 ,  C11C1/02 ,  C07C29/147 ,  C11C3/10 ,  C12N1/21 ,  C12P7/16

CPC classification number: C12P7/16 ,  A23K10/38 ,  B01D3/002 ,  B01D11/0492 ,  B01D21/26 ,  B01D21/262 ,  C07C29/86 ,  C11B1/025 ,  C11B13/00 ,  C12F3/10 ,  C12M1/00 ,  C12M21/12 ,  C12M45/04 ,  C12M45/09 ,  C12N1/14 ,  C12N15/00 ,  C12P7/18 ,  C12P7/6418 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02W30/74

Abstract: In an alcohol fermentation process, oil derived from biomass is chemically converted into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be chemically converted into a reaction product, such as fatty acids, fatty alcohols, fatty amides, fatty acid methyl esters, fatty acid glycol esters, and hydroxylated triglycerides, and mixtures thereof, which forms a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be chemically converting into the fermentation product extractant. The oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be chemically converted to a fermentation product extractant, which can then contacted with a fermentation product comprising a product alcohol, whereby the product alcohol is separated from the fermentation product.

Abstract translation: 在酒精发酵过程中，将来自生物质的油化学转化成可从发酵液中原位除去产物醇如丁醇的萃取剂。 油中的甘油酯可以化学转化成反应产物，例如脂肪酸，脂肪醇，脂肪酰胺，脂肪酸甲酯，脂肪酸二醇酯和羟基化甘油三酸酯及其混合物，其形成发酵产物提取剂，其具有 产物醇的分配系数大于产物醇的生物量的油的分配系数。 来自醇发酵方法的原料的油可以化学转化成发酵产物萃取剂。 在将原料进料到发酵容器之前，油可以与原料分离，并且将分离的油化学转化成发酵产物萃取剂，然后可将其与包含产物醇的发酵产物接触，由此产物醇 与发酵产物分离。

公开(公告)号：US20110212495A1

公开(公告)日：2011-09-01

申请号：US12871976

申请日：2010-08-31

Applicant: BRUCE A. DINER

Inventor： BRUCE A. DINER

IPC: C12P1/00 ,  C12M1/40 ,  C12P19/14

CPC classification number: C12N1/22 ,  C12M29/04 ,  C12M29/16 ,  C12M29/18 ,  C12M45/09 ,  C12P7/10 ,  C12P19/14 ,  C12Y302/01004 ,  Y02E50/16 ,  Y02E50/17

Abstract: Methods and apparatus' are disclosed for the simultaneous saccharification and fermentation of biomass providing for the compartmentalization of the saccharification process and the fermentation process resulting in decreased enzymatic end-product inhibition.

Abstract translation: 公开了用于同时糖化和发酵生物质的方法和装置，其提供了糖化过程的分隔和发酵过程，导致酶终止产物抑制的减少。