Nitric oxide as an activator of the plant pathogen defense systems
    1.
    发明授权
    Nitric oxide as an activator of the plant pathogen defense systems 有权
    一氧化氮作为植物病原体防御系统的活化剂

    公开(公告)号:US06198020B1

    公开(公告)日:2001-03-06

    申请号:US09257541

    申请日:1999-02-24

    IPC分类号: A01H100

    摘要: Compositions and methods to aid in protecting the plant from invading pathogenic organisms are provided. The compositions of the invention comprise genes that influence the levels of nitric oxide in plant cells. Such genes include those encoding nitric oxide synthase (iNOS) as well as natural resistance-associated macrophage proteins (NRAMP) and NRAMP homologues. A pathogen inducible promoter or alternatively a constitutive, preferably a weak constitutive, promoter is used to control the desired level of disease control in the plant. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.

    摘要翻译: 提供了有助于保护植物免受入侵病原生物体的组合物和方法。 本发明的组合物包含影响植物细胞中一氧化氮水平的基因。 这些基因包括编码一氧化氮合酶(iNOS)以及天然抗性相关巨噬细胞蛋白(NRAMP)和NRAMP同源物的基因。 病原体诱导型启动子或选择性的组成型优选弱组成型启动子用于控制植物中所需的疾病控制水平。 还提供了具有增强的抗病性的转化植物,植物细胞,组织和种子。

    Viral promoter, truncations thereof, and methods of use

    公开(公告)号:US08350121B2

    公开(公告)日:2013-01-08

    申请号:US12775600

    申请日:2010-05-07

    IPC分类号: C12N15/82 C12N15/113 C12N5/10

    CPC分类号: C12N15/8216

    摘要: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

    Antifungal polypeptides
    5.
    发明授权
    Antifungal polypeptides 有权
    抗真菌多肽

    公开(公告)号:US08148510B2

    公开(公告)日:2012-04-03

    申请号:US12795770

    申请日:2010-06-08

    IPC分类号: C12N15/82 A01H1/00

    摘要: Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

    摘要翻译: 提供了用于保护植物免受病原体,特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物,以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物,植物细胞,种子和微生物。