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    MICROFLUIDIC DROPLETS FOR METABOLIC ENGINEERING AND OTHER APPLICATIONS
    1.
    发明申请
    MICROFLUIDIC DROPLETS FOR METABOLIC ENGINEERING AND OTHER APPLICATIONS 审中-公开
    用于代谢工程和其他应用的微流控溅射

    公开(公告)号：US20100124759A1

    公开(公告)日：2010-05-20

    申请号：US12492830

    申请日：2009-06-26

    申请人： Benjamin L. Wang ,  Katherine J. Humphry ,  David A. Weitz ,  Gregory Stephanopoulos

    发明人： Benjamin L. Wang ,  Katherine J. Humphry ,  David A. Weitz ,  Gregory Stephanopoulos

    IPC分类号： C12Q1/28 ,  C12N1/18 ,  C12Q1/26 ,  C12N1/20

    CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421

    摘要： The present invention relates generally to the use of droplets to culture and/or assay cells or other species. In some cases, the cells or other species may be sorted based upon the results of the culture and/or assay. In some embodiments, cells other species can be encapsulated in droplets and exposed to one or more agents (e.g., a sugar, an indicator dye, etc.). For instance, in some cases, exposure of cells to the agents may result in the production of metabolites or other compounds (e.g., amino acids, proteins, organic acids, etc.) which may be, for example, assayed or otherwise determined. In some embodiments, the reaction of an agent with cells and/or other species within a droplet may reveal a property of the cells or other species (e.g., sugar consumption, growth rate, ability to withstand exposure to the agent, etc.). As an example, cells that produce desired metabolites or exhibit certain properties may be separated from the other cells via sorting techniques. Other aspects of the invention relate to devices or kits for implementing such sorts, methods of promoting such techniques, or the like.

    摘要翻译： 本发明一般涉及使用液滴来培养和/或测定细胞或其它物种。 在一些情况下，可以基于培养和/或测定的结果对细胞或其它物种进行分类。 在一些实施方案中，细胞其他物质可以被包封在液滴中并暴露于一种或多种试剂（例如，糖，指示剂染料等）。 例如，在一些情况下，细胞暴露于试剂可导致生成代谢物或其它化合物（例如，氨基酸，蛋白质，有机酸等），其可以例如被测定或以其他方式测定。 在一些实施方案中，药剂与细胞和/或液滴内的其它物质的反应可以揭示细胞或其他物种的性质（例如，糖消耗，生长速率，耐受试剂暴露的能力等）。 作为实例，产生所需代谢物或显示某些特性的细胞可以通过分选技术与其他细胞分离。 本发明的其他方面涉及用于实现这种类型的装置或套件，促进这种技术的方法等。

    Method of fabricating a narrow projection such as a write pole extending from a substrate
    3.
    发明授权
    Method of fabricating a narrow projection such as a write pole extending from a substrate 失效
    制造诸如从基板延伸的写极的窄突起的方法

    公开(公告)号：US06859998B2

    公开(公告)日：2005-03-01

    申请号：US10121989

    申请日：2002-04-12

    申请人： James Kruger ,  Benjamin L. Wang ,  Patrick R. Webb ,  Howard G. Zolla

    发明人： James Kruger ,  Benjamin L. Wang ,  Patrick R. Webb ,  Howard G. Zolla

    IPC分类号： G11B5/187 ,  G11B5/31 ,  B44C1/22 ,  C23C14/04

    CPC分类号： G11B5/1871 ,  G11B5/3116 ,  G11B5/3163 ,  Y10S29/016 ,  Y10T29/49032 ,  Y10T29/49041 ,  Y10T29/49043 ,  Y10T29/49046 ,  Y10T29/49052 ,  Y10T428/1186 ,  Y10T428/24174 ,  Y10T428/24479 ,  Y10T428/2462

    摘要： An article is formed as a substrate having a projection extending outwardly therefrom. The article may be a magnetic recording head and the projection a write pole. The projection has a width in a thinnest dimension measured parallel to a substrate surface of no more than about 0.3 micrometers and a height measured perpendicular to the substrate of not less than about 5 times the width. The article is fabricated by forming an overlying structure on the substrate with an edge thereon, depositing a replication layer lying on the edge, depositing a filler onto the edge and the substrate, so that the filler, the replication layer, and the overlying structure in combination comprise a continuous layer on the substrate, selectively removing at least a portion of the replication layer from a free surface of the continuous layer inwardly toward the substrate, to form a defined cavity, and depositing a projection material into the defined cavity to form the projection.

    摘要翻译： 形成具有从其向外延伸的突起的基底的制品。 物品可以是磁记录头，并且投影是写柱。 突起具有平行于衬底表面测量的最薄尺寸的宽度不大于约0.3微米，垂直于衬底的高度不小于宽度的约5倍。 通过在其上具有边缘的衬底上形成覆盖结构来制造制品，沉积位于边缘上的复制层，在边缘和衬底上沉积填料，使得填料，复制层和上覆结构在 组合包括在衬底上的连续层，选择性地将复制层的至少一部分从连续层的自由表面向内移动到衬底，以形成限定的空腔，并将突出材料沉积到限定的空腔中以形成 投影。

    Method of fabricating a narrow projection such as a write pole extending from a substrate
    4.
    发明授权
    Method of fabricating a narrow projection such as a write pole extending from a substrate 失效
    制造诸如从基板延伸的写极的窄突起的方法

    公开(公告)号：US07355813B2

    公开(公告)日：2008-04-08

    申请号：US11053632

    申请日：2005-02-08

    申请人： James Kruger ,  Benjamin L. Wang ,  Patrick R. Webb ,  Howard G. Zolla

    发明人： James Kruger ,  Benjamin L. Wang ,  Patrick R. Webb ,  Howard G. Zolla

    IPC分类号： G11B5/31 ,  G11B5/127 ,  H04R31/00

    CPC分类号： G11B5/1871 ,  G11B5/3116 ,  G11B5/3163 ,  Y10S29/016 ,  Y10T29/49032 ,  Y10T29/49041 ,  Y10T29/49043 ,  Y10T29/49046 ,  Y10T29/49052 ,  Y10T428/1186 ,  Y10T428/24174 ,  Y10T428/24479 ,  Y10T428/2462

    摘要： An article is formed as a substrate having a projection extending outwardly therefrom. The article may be a magnetic recording head and the projection a write pole. The projection has a width in a thinnest dimension measured parallel to a substrate surface of no more than about 0.3 micrometers and a height measured perpendicular to the substrate of not less than about 5 times the width. The article is fabricated by forming an overlying structure on the substrate with an edge thereon, depositing a replication layer lying on the edge, depositing a filler onto the edge and the substrate, so that the filler, the replication layer, and the overlying structure in combination comprise a continuous layer on the substrate, selectively removing at least a portion of the replication layer from a free surface of the continuous layer inwardly toward the substrate, to form a defined cavity, and depositing a projection material into the defined cavity to form the projection.

    摘要翻译： 形成具有从其向外延伸的突起的基底的制品。 物品可以是磁记录头，并且投影是写柱。 突起具有平行于衬底表面测量的最薄尺寸的宽度不大于约0.3微米，并且垂直于衬底测量的高度不小于宽度的约5倍。 通过在其上具有边缘的衬底上形成覆盖结构来制造制品，沉积位于边缘上的复制层，在边缘和衬底上沉积填料，使得填料，复制层和上覆结构在 组合包括在衬底上的连续层，选择性地将复制层的至少一部分从连续层的自由表面向内移动到衬底，以形成限定的空腔，并将突出材料沉积到限定的空腔中以形成 投影。