Selective isotopic labeling of spin labels for electron spin resonance
spectroscopy
    9.
    发明授权
    Selective isotopic labeling of spin labels for electron spin resonance spectroscopy 失效
    电子自旋共振光谱自旋标记的选择性同位素标记

    公开(公告)号:US5431901A

    公开(公告)日:1995-07-11

    申请号:US227793

    申请日:1988-08-03

    摘要: Selective isotopic-labeling of spin label compounds, used in electron spin resonance spectroscopy to detect, measure and monitor the presence of paramagnetic species, increases the sensitivity of the electron spin resonance technique in assays for paramagnetic species, such as the determination of oxygen tension in solution or in a biological sample, like a living organism. Spin label compounds, such as nitroxides, like 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl (CTPO), are selectively, but not completely, isotopically-labeled, such as partially deuterated, to increase the sensitivity and reliability of assays employing electron spin resonance spectroscopic measurements.

    摘要翻译: 用于电子自旋共振光谱法的自旋标记化合物的选择性同位素标记用于检测,测量和监测顺磁性物质的存在,增加了电子自旋共振技术在顺磁性物质的测定中的灵敏度,例如测定氧气张力 溶液或生物样品,如活体。 旋转标记化合物,例如硝基氧,如3-氨基甲酰基-2,2,5,5-四甲基-3-吡咯啉-1-基氧基(CTPO),是选择性但不完全同位素标记的,例如部分氘代, 以提高使用电子自旋共振光谱测量的测定的灵敏度和可靠性。