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公开(公告)号：US07385040B2
公开(公告)日：2008-06-10
申请号：US10589718
申请日：2005-02-24
申请人： Bo-Lennart Johansson , Hans J. Johansson , Anders Ljunglöf , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans J. Johansson , Anders Ljunglöf , Jean-Luc Maloisel , Nicolas Thevenin
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译： 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。
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公开(公告)号：US07750129B2
公开(公告)日：2010-07-06
申请号：US10589717
申请日：2005-02-25
申请人： Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans J. Johansson , Anna Grönberg , Jean-Luc Maloisel , Nicolas Thevenin
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译： 本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到 所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。 在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。
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公开(公告)号：US20060175258A1
公开(公告)日：2006-08-10
申请号：US10547570
申请日：2004-03-05
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译： 本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。 本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。
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公开(公告)号：US07320755B2
公开(公告)日：2008-01-22
申请号：US10547570
申请日：2004-03-05
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译： 本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。 本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。
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公开(公告)号：US20070167613A1
公开(公告)日：2007-07-19
申请号：US10589717
申请日：2005-02-25
IPC分类号： C07K16/18
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译： 本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到 所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。 在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。
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公开(公告)号：US20070112178A1
公开(公告)日：2007-05-17
申请号：US10589718
申请日：2005-02-24
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译： 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。
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公开(公告)号：US07510657B2
公开(公告)日：2009-03-31
申请号：US10547569
申请日：2004-03-05
CPC分类号： B01J20/286 , B01D15/3804 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3255 , B01J20/3259
摘要： The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatize the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatized scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.
摘要翻译： 本发明是制备亲和配体的方法，该方法包括提供包含硫醇基，羰基和胺基的环状支架; 以亲电子试剂衍生所述支架的胺基团，所述亲电子试剂携带能够进行亲和相互作用的官能团或能够与靶分子进行第二相互作用的官能团; 并开启衍生的支架并加入携带能够进行第二相互作用的官能团或能够与靶分子亲和相互作用的官能团的胺。 该方法提供了提供亲和力配体，其能够进行亲和力相互作用以及第二种相互作用。
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公开(公告)号：US08217118B2
公开(公告)日：2012-07-10
申请号：US12094286
申请日：2006-11-22
申请人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07D303/02 , C07H1/00 , C08F8/00
CPC分类号： B01J20/262 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28016 , B01J20/286 , B01J20/287 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3217 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J2220/54 , B01J2220/58
摘要： Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.
摘要翻译： 公开了一种利用相转移催化条件活化具有环氧基团的固体支持材料和固定配体的方法。 该方法允许以高水平的取代在载体材料上引入环氧基团和特定的亲核配体。 此外，本发明提供了用于固定配体以用于各种色谱分离方法如离子交换层析，疏水相互作用层析（HIC），反相色谱（RPC）或亲和层析中的一般方法。
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公开(公告)号：US08648146B2
公开(公告)日：2014-02-11
申请号：US13489492
申请日：2012-06-06
申请人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C08F8/00 , C07H1/00 , C07D303/02
CPC分类号： B01J20/262 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28016 , B01J20/286 , B01J20/287 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3217 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J2220/54 , B01J2220/58
摘要： Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.
摘要翻译： 公开了一种利用相转移催化条件活化具有环氧基团的固体支持材料和固定配体的方法。 该方法允许以高水平的取代在载体材料上引入环氧基团和特定的亲核配体。 此外，本发明提供了用于固定配体以用于各种色谱分离方法如离子交换层析，疏水相互作用层析（HIC），反相色谱（RPC）或亲和层析中的一般方法。
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公开(公告)号：US07320754B2
公开(公告)日：2008-01-22
申请号：US10547567
申请日：2004-03-05
IPC分类号： B01D15/08
CPC分类号： B01J20/3242 , B01D15/363 , B01J20/289 , B01J20/3219 , B01J20/3285 , B01J41/12 , B01J41/20
摘要： The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.
摘要翻译： 本发明涉及制备多模式阴离子交换配体的方法，其包括提供包含胺，羰基和硫醇的环状三官能支架; 可任选地衍生化支架的胺以提供阴离子交换基团和/或保护胺; 并通过加入包含偶联到残基R的胺的试剂以将所述胺加入到开放的支架的羰基碳中，开启衍生物。 支架有利的是同型半胱氨酸硫内酯。 在一个实施方案中，该方法包括将开放的支架固定在基础基质上以提供分离介质如色谱介质的附加步骤。
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