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    Antibody purification
    1.
    发明授权
    Antibody purification 有权
    抗体纯化

    公开(公告)号：US07385040B2

    公开(公告)日：2008-06-10

    申请号：US10589718

    申请日：2005-02-24

    申请人： Bo-Lennart Johansson ,  Hans J. Johansson ,  Anders Ljunglöf ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Hans J. Johansson ,  Anders Ljunglöf ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： C07K1/18 ,  C07K1/22

    CPC分类号： C07K1/18 ,  B01D15/362 ,  B01D15/3809 ,  B01D15/3847 ,  B01J39/07 ,  B01J39/26 ,  B01J47/014 ,  C07K1/165 ,  B01D15/327

    摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.

    摘要翻译： 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

    Process for the purification of antibodies
    2.
    发明授权
    Process for the purification of antibodies 有权
    抗体纯化方法

    公开(公告)号：US07750129B2

    公开(公告)日：2010-07-06

    申请号：US10589717

    申请日：2005-02-25

    申请人： Bo-Lennart Johansson ,  Hans J. Johansson ,  Anna Grönberg ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Hans J. Johansson ,  Anna Grönberg ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： C07K1/18 ,  C07K1/22

    CPC分类号： B01J20/3253 ,  B01D15/3847 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3255 ,  B01J20/3285

    摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.

    摘要翻译： 本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到 所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。 在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。

    Method of preparing ligands for hydrophobic interaction chromatography
    3.
    发明申请
    Method of preparing ligands for hydrophobic interaction chromatography 失效
    制备疏水相互作用色谱配体的方法

    公开(公告)号：US20060175258A1

    公开(公告)日：2006-08-10

    申请号：US10547570

    申请日：2004-03-05

    申请人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： C07F5/04 ,  B01D15/08

    CPC分类号： B01J20/3285 ,  B01D15/325 ,  B01D15/327 ,  B01J20/286

    摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.

    摘要翻译： 本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。 本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

    Method of preparing ligands for hydrophobic interaction chromatography
    4.
    发明授权
    Method of preparing ligands for hydrophobic interaction chromatography 失效
    制备疏水相互作用色谱配体的方法

    公开(公告)号：US07320755B2

    公开(公告)日：2008-01-22

    申请号：US10547570

    申请日：2004-03-05

    申请人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： B01D15/08

    CPC分类号： B01J20/3285 ,  B01D15/325 ,  B01D15/327 ,  B01J20/286

    摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.

    摘要翻译： 本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。 本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

    Process for the purification of antibodies
    5.
    发明申请
    Process for the purification of antibodies 有权
    抗体纯化方法

    公开(公告)号：US20070167613A1

    公开(公告)日：2007-07-19

    申请号：US10589717

    申请日：2005-02-25

    申请人： Bo-Lennart Johansson ,  Hans Johansson ,  Anna Gronberg ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Hans Johansson ,  Anna Gronberg ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： C07K16/18

    CPC分类号： B01J20/3253 ,  B01D15/3847 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3255 ,  B01J20/3285

    摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.

    摘要翻译： 本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到 所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。 在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。

    Antibody purification
    6.
    发明申请
    Antibody purification 有权
    抗体纯化

    公开(公告)号：US20070112178A1

    公开(公告)日：2007-05-17

    申请号：US10589718

    申请日：2005-02-24

    申请人： Bo-Lennart Johansson ,  Hans Johansson ,  Anders Ljunglof ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    发明人： Bo-Lennart Johansson ,  Hans Johansson ,  Anders Ljunglof ,  Jean-Luc Maloisel ,  Nicolas Thevenin

    IPC分类号： C07K16/00 ,  C02F1/28

    CPC分类号： C07K1/18 ,  B01D15/362 ,  B01D15/3809 ,  B01D15/3847 ,  B01J39/07 ,  B01J39/26 ,  B01J47/014 ,  C07K1/165 ,  B01D15/327

    摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.

    摘要翻译： 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。