公开(公告)号：US09657304B2

公开(公告)日：2017-05-23

申请号：US13938784

申请日：2013-07-10

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Sanjaya

IPC分类号： C12N15/82 ,  A01H5/10 ,  C07K14/415

CPC分类号： C12N15/8247 ,  C07K14/415 ,  C12N9/1241 ,  C12N15/8218 ,  C12N15/8245 ,  C12Y207/07027

摘要： The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.

公开(公告)号：US09062331B2

公开(公告)日：2015-06-23

申请号：US13719868

申请日：2012-12-19

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Rachel Miller ,  Eric R. Moellering

IPC分类号： C12N15/82 ,  C07H21/04 ,  C12P7/64 ,  C12N9/10 ,  C12N15/79

CPC分类号： C12P7/6463 ,  C12N9/1029 ,  C12N15/79 ,  C12N15/8247 ,  C12P7/6409 ,  C12Y203/0102

摘要： The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chiarella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

摘要翻译： 本发明涉及生物合成油组合物及其制备方法。 在一些实施方案中，本发明涉及内源性酶在能够合成油的植物中的用途。 在优选的实施方案中，所述植物是藻类。 在另外的实施方案中，所述藻类来自衣藻，南非氯氰菊酯，杜氏藻属，赤霉病菌属和Scenedesmus。 在另外的实施方案中，所述内源酶是二酰基甘油酰基转移酶。

公开(公告)号：US20140134685A1

公开(公告)日：2014-05-15

申请号：US14073959

申请日：2013-11-07

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Xiaobo Li ,  Bensheng Liu ,  Min-Hao Kuo ,  Barbara Sears

IPC分类号： C12P7/64 ,  C12N15/82

CPC分类号： C12P7/6463 ,  C12N15/8247 ,  Y02E50/13

摘要： The invention provides a recombinant cell having a nucleotide sequence encoding a polypeptide which is a lipase having at least 40% amino acid sequence identity to a polypeptide having SEQ ID NO:1, and methods of using the recombinant cell to produce triacylglycerols or to increase oil production by the cell.

摘要翻译： 本发明提供了具有编码多肽的核苷酸序列的重组细胞，该多肽是与具有SEQ ID NO：1的多肽具有至少40％氨基酸序列同一性的脂肪酶，以及使用该重组细胞产生三酰基甘油或增加油的方法 由细胞生产。

公开(公告)号：US11286494B2

公开(公告)日：2022-03-29

申请号：US16498663

申请日：2018-03-29

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Kun Wang

IPC分类号： C12N15/82 ,  C12N9/02 ,  C12N9/20

摘要： Described herein are transgenic plants with increased oil content that exhibit enhanced expression of plastid-specific lipases (e.g., PLIP1). The manufacture of lipids can be enhanced by expression of FAD4.

公开(公告)号：US10858687B2

公开(公告)日：2020-12-08

申请号：US16058632

申请日：2018-08-08

申请人： Board of Trustees of Michigan State University

发明人： Gregory Bonito ,  Zhi-Yan Du ,  Christoph Benning

IPC分类号： C12P39/00 ,  C12N1/12 ,  C12P7/64 ,  C12N9/02 ,  C12N1/14 ,  A01H15/00 ,  C12N9/00 ,  C12N15/82 ,  C12R1/85 ,  C12R1/885 ,  C12R1/89 ,  C12R1/77 ,  C12R1/80 ,  C12R1/66 ,  C12R1/72 ,  C12R1/84

摘要： This application describes consortium between fungi and algae, where the algae are incorporated within hyphae of the fungi. The fungi, the algae, or both can be modified to express heterologous lipid synthesizing enzymes. Incorporation of algae into fungi facilitates harvesting of the algae and products produced by the consortia. Such consortia are robust. For example, the fungi and algae can symbiotically provide nutrients to each other and are tolerant of environmental stresses.

公开(公告)号：US10421975B2

公开(公告)日：2019-09-24

申请号：US15627006

申请日：2017-06-19

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  John B. Ohlrogge ,  Wei Ma ,  Que Kong

IPC分类号： C12N15/82 ,  C07K14/415

摘要： The application describes plants, plant cells, and seeds that express WRINKLED1 transcription factors and/or 14-3-3 proteins that are useful for increasing production of oils in plants and seeds. Also described are expression systems and cassettes that encode and express such WRINKLED1 transcription factors and/or 14-3-3 proteins.

公开(公告)号：US10280432B2

公开(公告)日：2019-05-07

申请号：US15487063

申请日：2017-04-13

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Sanjaya

IPC分类号： C12N15/82 ,  C12N9/12 ,  C07K14/415

摘要： The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.

公开(公告)号：US20140020133A1

公开(公告)日：2014-01-16

申请号：US13938784

申请日：2013-07-10

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Sanjaya

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C07K14/415 ,  C12N9/1241 ,  C12N15/8218 ,  C12N15/8245 ,  C12Y207/07027

摘要： The invention relates to genetically modified agricultural plants with increased oil content in vegetative tissues, as well as to expression systems, plant cells, seeds and vegetative tissues related thereto.

摘要翻译： 本发明涉及营养组织中油含量增加的转基因农作物以及与其相关的表达系统，植物细胞，种子和营养组织。

公开(公告)号：US10570404B2

公开(公告)日：2020-02-25

申请号：US15199357

申请日：2016-06-30

申请人： Board of Trustees of Michigan State University

发明人： John B. Ohlrogge ,  Christoph Benning ,  Wei Ma ,  Que Kong

IPC分类号： C12N15/82 ,  C07K14/415

摘要： The application describes stabilized WRINKLED1 transcription factors, as well as nucleic acids, and expression cassettes that encode and express such stabilized WRINKLED1 transcription factors and that are useful for increasing production of oils in plants and seeds.

公开(公告)号：US10392629B2

公开(公告)日：2019-08-27

申请号：US14598953

申请日：2015-01-16

申请人： Board of Trustees of Michigan State University

发明人： Christoph Benning ,  Sanjaya ,  Rachel Miller

IPC分类号： C12N15/82 ,  C12N9/10 ,  C10L1/19 ,  C10G32/00

摘要： As described herein, plants expressing algal Diacylglycerol Acyltransferase Type Two (DGTT) from heterologous nucleic acids can alter acyl carbon partitioning in plant vegetative tissues and increase acyl-CoA-dependent triacylglycerol synthesis, thereby increasing the lipid content of the plants' tissues.