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    Methods and compositions for labeling nucleic acids
    4.
    发明申请
    Methods and compositions for labeling nucleic acids 有权
    用于标记核酸的方法和组合物

    公开(公告)号：US20110065907A1

    公开(公告)日：2011-03-17

    申请号：US12914338

    申请日：2010-10-28

    申请人： Adrian Salic ,  Timothy J. Mitchison

    发明人： Adrian Salic ,  Timothy J. Mitchison

    IPC分类号： C07H21/04 ,  C07H1/00

    CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. In particular, the methods include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    摘要翻译： 本发明涉及体外和体内标记核酸聚合物的方法。 特别地，所述方法包括掺入核酸聚合物的核苷酸类似物与附着于标签的试剂之间的[3 + 2]环加成。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS
    5.
    发明申请
    METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS 有权
    标记核酸的方法和组合物

    公开(公告)号：US20100311063A1

    公开(公告)日：2010-12-09

    申请号：US12785999

    申请日：2010-05-24

    申请人： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    发明人： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    摘要翻译： 本发明涉及体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

    Methods and compositions for labeling nucleic acids
    6.
    发明授权
    Methods and compositions for labeling nucleic acids 有权
    用于标记核酸的方法和组合物

    公开(公告)号：US07767421B2

    公开(公告)日：2010-08-03

    申请号：US11588697

    申请日：2006-10-27

    申请人： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    发明人： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC分类号： C12P19/34 ,  C07H21/02

    CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    摘要翻译： 本发明涉及体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。

    Detecting structural or synthetic information about chemical compounds
    9.
    发明授权
    Detecting structural or synthetic information about chemical compounds 失效
    检测关于化合物的结构或综合信息

    公开(公告)号：US06541203B2

    公开(公告)日：2003-04-01

    申请号：US09448395

    申请日：1999-11-23

    申请人： Timothy J. Mitchison

    发明人： Timothy J. Mitchison

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6816 ,  C12Q1/686 ,  G01N33/543 ,  G01N33/582 ,  C12Q2565/626 ,  C12Q2563/185 ,  C12Q2563/131

    摘要： The present invention provides an improved system for the rapid and non-destructive identification of chemical compounds attached to solid supports. In general, the invention provides an identification unit comprising a tag attached to the solid support and a binding partner that interacts specifically and detectably with he tag. In preferred embodiments, the identification unit incorporates the advantages of chemically robust tags and a decoding technique capable of amplification for easy detection and analysis. The present invention further provides a kit comprising a collection of tags capable of attachment to a support and binding partners capable of binding selectively and detectably to the tags, to generate an identification unit for the facile determination of the structure of a compound of interest by determining the reaction history and/or structural characteristics of the compounds that are encoded by the identification unit.

    摘要翻译： 本发明提供了用于快速和非破坏性鉴定连接到固体支持物上的化合物的改进的系统。 通常，本发明提供了一种识别单元，其包括附接到固体支持物的标签和与标签特异性和可检测地相互作用的结合配偶体。 在优选实施例中，识别单元结合了化学稳健标签的优点和能够进行放大以便于检测和分析的解码技术。 本发明还提供了一种试剂盒，其包括能够附着于能够选择性地和可检测地结合标签的载体和结合配偶体的标签的集合，以产生识别单元，以通过确定感兴趣的化合物的结构来容易地确定感兴趣的化合物的结构 由识别单元编码的化合物的反应历程和/或结构特征。

    Methods and compositions for labeling nucleic acids
    10.
    发明授权
    Methods and compositions for labeling nucleic acids 有权
    用于标记核酸的方法和组合物

    公开(公告)号：US08541570B2

    公开(公告)日：2013-09-24

    申请号：US12785999

    申请日：2010-05-24

    申请人： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    发明人： Kyle R. Gee ,  Brian Agnew ,  Adrian Salic ,  Timothy J. Mitchison

    IPC分类号： C07H19/06 ,  C07F9/02 ,  G01N33/48

    CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N33/5011 ,  G01N33/582 ,  C12Q2525/101 ,  C12Q2563/107

    摘要： The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.

    摘要翻译： 本发明涉及体外和体内标记核酸聚合物的方法。 提供了包括掺入核酸聚合物的核苷酸类似物与附着于标记物上的试剂之间的[3 + 2]环加成的某些方法。 提供了包括掺入核酸聚合物的核苷酸类似物与包含连接到标签上的取代的三芳基膦的试剂之间的施陶丁格连接的其它方法。 这种方法不需要固定和变性，因此可以应用于活细胞和生物体中核酸聚合物的标记。 还提供了测量细胞增殖的方法。 在这些方法中，测量结合到DNA中的标记量作为细胞增殖的指示。 本发明的方法可用于各种应用，包括涉及细胞增殖的疾病和病症的临床诊断，毒性测定以及用于研究染色体超微结构的工具。