Trimerizing polypeptides
    1.
    发明授权
    Trimerizing polypeptides 有权
    三聚多肽

    公开(公告)号:US08084230B2

    公开(公告)日:2011-12-27

    申请号:US12686896

    申请日:2010-01-13

    IPC分类号: C12P21/06

    摘要: The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

    摘要翻译: 本发明涉及一种三聚蛋白的制备方法,其包括用编码包含ZymoZipper(ZZ)结构域和异源蛋白质的融合蛋白的表达载体转化或转染的宿主细胞。 在一个实施方案中,异源蛋白质是膜蛋白,融合蛋白中包含的异源蛋白质的部分是该蛋白质的细胞外结构域,并且所得的融合蛋白质是可溶的。 在本发明的另一个实施方案中,ZZ结构域衍生自病毒包膜蛋白的跨膜(TM)亚基或其它含有病毒基因组的七重复重复基因。 该方法可用于产生同型和异三聚体蛋白质。 本发明还包括本方法中使用的DNA分子,表达载体和宿主细胞以及通过本发明方法产生的融合蛋白。

    TRIMERIZING POLYPEPTIDES
    2.
    发明申请
    TRIMERIZING POLYPEPTIDES 有权
    调整多糖

    公开(公告)号:US20100297701A1

    公开(公告)日:2010-11-25

    申请号:US12686896

    申请日:2010-01-13

    IPC分类号: C12P21/06

    摘要: The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

    摘要翻译: 本发明涉及一种三聚蛋白的制备方法,其包括用编码包含ZymoZipper(ZZ)结构域和异源蛋白质的融合蛋白的表达载体转化或转染的宿主细胞。 在一个实施方案中,异源蛋白质是膜蛋白,融合蛋白中包含的异源蛋白质的部分是该蛋白质的细胞外结构域,并且所得的融合蛋白质是可溶的。 在本发明的另一个实施方案中,ZZ结构域衍生自病毒包膜蛋白的跨膜(TM)亚基或其它含有病毒基因组的七重复重复基因。 该方法可用于产生同型和异三聚体蛋白质。 本发明还包括本方法中使用的DNA分子,表达载体和宿主细胞以及通过本发明方法产生的融合蛋白。

    MATERIALS AND METHODS FOR PREPARING DIMERIC GROWTH FACTORS
    6.
    发明申请
    MATERIALS AND METHODS FOR PREPARING DIMERIC GROWTH FACTORS 审中-公开
    制备二元生长因子的材料与方法

    公开(公告)号:US20090280562A1

    公开(公告)日:2009-11-12

    申请号:US12494050

    申请日:2009-06-29

    IPC分类号: C12N15/63 C12N5/10

    摘要: Proteins consisting of, from amino to carboxyl terminus, a first PDGF-D growth factor domain polypeptide, a linker polypeptide, and a second PDGF-D growth factor domain polypeptide, and materials and methods for making the proteins are disclosed. Each of the first and second PDGF-D growth factor domain polypeptides consists of a sequence of amino acid residues as shown in SEQ ID NO:2 or SEQ ID NO:4 from amino acid x to amino acid y, wherein x is an integer from 246 to 258, inclusive, and y is an integer from 365-370, inclusive. The linker polypeptide consists of from 11-40 amino acid residues. The proteins can be used to stimulate the production of bone and/or connective tissue in both humans and non-human animals.

    摘要翻译: 公开了由氨基至羧基末端组成第一PDGF-D生长因子结构域多肽,接头多肽和第二PDGF-D生长因子结构域多肽的蛋白质,以及用于制备蛋白质的材料和方法。 第一和第二PDGF-D生长因子结构域多肽中的每一个由从氨基酸x至氨基酸y的SEQ ID NO:2或SEQ ID NO:4所示的氨基酸残基序列组成,其中x是 246至258(含),y为365-370的整数,包括端值。 接头多肽由11-40个氨基酸残基组成。 蛋白质可用于刺激人和非人动物中骨和/或结缔组织的产生。

    Materials and methods for preparing dimeric growth factors
    7.
    发明授权
    Materials and methods for preparing dimeric growth factors 失效
    制备二聚生长因子的材料和方法

    公开(公告)号:US07556941B2

    公开(公告)日:2009-07-07

    申请号:US11550155

    申请日:2006-10-17

    摘要: Proteins consisting of, from amino to carboxyl terminus, a first PDGF-D growth factor domain polypeptide, a linker polypeptide, and a second PDGF-D growth factor domain polypeptide, and materials and methods for making the proteins are disclosed. Each of the first and second PDGF-D growth factor domain polypeptides consists of a sequence of amino acid residues as shown in SEQ ID NO: 2 or SEQ ID NO: 4 from amino acid x to amino acid y, wherein x is an integer from 246 to 258, inclusive, and y is an integer from 365-370, inclusive. The linker polypeptide consists of from 11-40 amino acid residues. The proteins can be used to stimulate the production of bone and/or connective tissue in both humans and non-human animals.

    摘要翻译: 公开了由氨基至羧基末端组成第一PDGF-D生长因子结构域多肽,接头多肽和第二PDGF-D生长因子结构域多肽的蛋白质,以及用于制备蛋白质的材料和方法。 第一和第二PDGF-D生长因子结构域多肽中的每一个由从氨基酸x至氨基酸y的SEQ ID NO:2或SEQ ID NO:4所示的氨基酸残基序列组成,其中x是 246至258(含),y为365-370的整数,包括端值。 接头多肽由11-40个氨基酸残基组成。 蛋白质可用于刺激人和非人动物中骨和/或结缔组织的产生。

    Kunitz domain polypeptide zkun10
    10.
    发明授权
    Kunitz domain polypeptide zkun10 有权
    Kunitz结构域多肽zkun10

    公开(公告)号:US06914135B2

    公开(公告)日:2005-07-05

    申请号:US10315432

    申请日:2002-12-09

    CPC分类号: C07K14/8114 A61K38/00

    摘要: Proteinase inhibitors comprising a Kunitz domain are disclosed. The Kunitz domain comprises a motif of amino acid residues as shown in SEQ ID NO:4, and the sequence of the Kunitz domain is shown in residues 57 through 107 of SEQ ID NO:2. The polypeptide also includes an N-terminal collagen domain in which a von Willebrand domain resides, and is shown in SEQ ID NO: 5. Also disclosed are methods for making the proteinase inhibitors, and expression vectors and cultured cells that are useful within the methods. The proteinase inhibitors may be used as components of cell culture media, in protein purification, and as inhibitors of protease degradation of plasma proteins.

    摘要翻译: 公开了包含Kunitz结构域的蛋白酶抑制剂。 Kunitz结构域包含如SEQ ID NO:4所示的氨基酸残基的基序,Kunitz结构域的序列显示于SEQ ID NO:2的残基57至107中。 多肽还包括其中von Willebrand结构域所在的N-末端胶原结构域,并且显示于SEQ ID NO:5中。还公开了制备蛋白酶抑制剂的方法,以及在该方法中有用的表达载体和培养细胞 。 蛋白酶抑制剂可以用作细胞培养基的组分,蛋白质纯化,以及作为血浆蛋白质蛋白酶降解的抑制剂。