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公开(公告)号：US06776988B2
公开(公告)日：2004-08-17
申请号：US10158735
申请日：2002-05-29
IPC分类号： A01N6300
CPC分类号： C12N15/85 , C12N15/1034 , C12N15/1037 , C12N15/1051 , C12N15/86 , C12N2740/10043 , C12N2740/13043 , C12N2800/30 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44 , C40B40/02
摘要： Novel vectors are described that incorporate, inter alia, a novel 3′ gene trap cassette which can be used to efficiently trap and identify previously unknown cellular genes. Vectors incorporating the described 3′ gene trap cassette find particular application in gene discovery and in the production of mutated cells and animals.
摘要翻译： 描述了新颖的载体，其特别包含一种新的3'基因捕获盒，其可以用于有效捕获和鉴定先前未知的细胞基因。 结合所述3'基因捕获盒的载体在基因发现和突变的细胞和动物的产生中具有特殊应用。
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公开(公告)号：US06808921B1
公开(公告)日：2004-10-26
申请号：US09443282
申请日：1999-11-19
IPC分类号： C12N1500
CPC分类号： C40B40/02 , C12N15/1034 , C12N15/1037 , C12N15/1051 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2740/13043 , C12N2800/30 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Novel vectors are described that incorporate, inter alia, a novel 3′ gene trap cassette that does not encode a marker conferring antibiotic resistance and which can be used to efficiently trap, mutagenize, and identify previously unknown cellular genes. Vectors incorporating the presently described 3′ gene trap cassette find particular application in gene discovery, and the production of mutated cells and animals.
摘要翻译： 描述了新颖的载体，其特别包含一种新颖的3'基因捕获盒，其不编码赋予抗生素抗性的标记，其可用于有效捕获，诱变和鉴定先前未知的细胞基因。 结合目前描述的3'基因陷阱盒的载体在基因发现中具有特殊应用，并且产生突变的细胞和动物。
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公开(公告)号：US06436707B1
公开(公告)日：2002-08-20
申请号：US09276533
申请日：1999-03-25
IPC分类号： C12N1500
CPC分类号： C12N15/85 , C12N15/1034 , C12N15/1037 , C12N15/1051 , C12N15/86 , C12N2740/10043 , C12N2740/13043 , C12N2800/30 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44 , C40B40/02
摘要： Novel vectors are described that incorporate, inter alia, a novel 3′ gene trap cassette which can be used to efficiently trap and identify previously unknown cellular genes. Vectors incorporating the described 3′ gene trap cassette find particular application in gene discovery and in the production of mutated cells and animals.
摘要翻译： 描述了新颖的载体，其特别包含一种新的3'基因捕获盒，其可用于有效捕获和鉴定先前未知的细胞基因。 结合所述3'基因捕获盒的载体在基因发现和突变的细胞和动物的产生中具有特殊应用。
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公开(公告)号：US06548271B1
公开(公告)日：2003-04-15
申请号：US09556916
申请日：2000-04-21
IPC分类号： C07H2102
CPC分类号： C07K14/705 , A01K2217/05
摘要： The present invention provides two novel families of novel human transporter proteins (NTPs). The invention additionally provides for agonists, antagonists, antibodies, antisense molecules that are specific for the NTPs, and further provides genetically engineered expression vectors for the NTPs and host comprising the same. The invention further provides for processes for identifying/producing molecules that effect NTP activity which comprise the use of the disclosed NTPs or genes encoding the same.
摘要翻译： 本发明提供两种新型人类转运蛋白（NTPs）的新家族。 本发明另外提供对NTP有特异性的激动剂，拮抗剂，抗体，反义分子，并进一步提供用于NTP和包含其的宿主的基因工程表达载体。 本发明进一步提供鉴定/产生影响NTP活性的分子的方法，其包括使用所公开的NTP或编码其的基因。
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5.发明申请Indexed library of cells containing genomic modifications and methods of making and utilizing the same 审中-公开指定的含有基因组修饰的细胞库，以及制备和利用它们的方法公开(公告)号：US20090149336A1
公开(公告)日：2009-06-11
申请号：US11978961
申请日：2007-10-29
IPC分类号： C40B30/04
CPC分类号： C12N15/1082 , A01K2217/075 , C12N15/102 , C12N15/1034 , C12N15/1051 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2800/60 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译： 提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。 该文库优选地在基因组中存在基因组中所有基因的突变。 图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。 这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。 本发明包括描述的库，制作方法和用于构建库的向量。 还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。 本发明还提供了从文库细胞中分离并产生的特异性基因突变的非人转基因动物的产生。
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6.发明授权Indexed library of cells containing genomic modifications and methods of making and utilizing the same 失效指定的含有基因组修饰的细胞库，以及制备和利用它们的方法公开(公告)号：US06207371B1
公开(公告)日：2001-03-27
申请号：US08942806
申请日：1997-10-02
IPC分类号： C12Q168
CPC分类号： C12N15/1082 , A01K2217/075 , C12N15/102 , C12N15/1034 , C12N15/1051 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2800/60 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译： 提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。 该文库优选地在基因组中存在基因组中所有基因的突变。 图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。 这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。 本发明包括描述的库，制作方法和用于构建库的向量。 还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。 本发明还提供了从文库细胞分离和产生的特异性基因突变的非人转基因动物的产生。
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公开(公告)号：US07332338B2
公开(公告)日：2008-02-19
申请号：US10917273
申请日：2004-08-11
CPC分类号： C12N15/86 , A01K2217/075 , C12N15/102 , C12N15/1051 , C12N15/1065 , C12N15/1086 , C12N15/85 , C12N2740/13043 , C12N2800/60 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译： 提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。 该文库优选地在基因组中存在基因组中所有基因的突变。 图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。 这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。 本发明包括描述的库，制作方法和用于构建库的向量。 还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。 本发明还提供了从文库细胞分离和产生的特异性基因突变的非人转基因动物的产生。
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8.发明授权Indexed library of cells containing genomic modifications and methods of making and utilizing the same 有权指定的含有基因组修饰的细胞库，以及制备和利用它们的方法公开(公告)号：US06855545B1
公开(公告)日：2005-02-15
申请号：US09639453
申请日：2000-08-15
IPC分类号： C12N15/10 , C12N15/85 , C12N15/867 , C12N15/86 , C12N15/00 , C12N15/09 , C12N15/82 , C12Q1/68
CPC分类号： C12N15/1082 , A01K2217/075 , C12N15/102 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the gene of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
摘要翻译： 提供方法和载体（DNA和逆转录病毒）用于构建突变细胞库。 该文库将优选地包含基因中存在于细胞基因中的所有基因的突变。 图书馆和载体的性质允许筛选特定基因突变的方法，并收集每个突变基因的核苷酸序列数据，以提供标记基因序列的数据库。 这样的数据库提供了访问图书馆中包含的各个突变细胞克隆的方法。 本发明包括描述的库，制作方法和用于构建库的向量。 还提供了通过序列或汇总和筛选来访问图书馆的各个部分的方法。 本发明还提供了从文库细胞分离和产生的特异性基因突变的非人转基因动物的产生。
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9.发明申请Genetically Engineered and Photyped Mice and Stem Cell Clones for Producing the Same 审中-公开基因工程和愚蠢的小鼠和干细胞克隆生产它公开(公告)号：US20090077680A1
公开(公告)日：2009-03-19
申请号：US12099465
申请日：2008-04-08
申请人： Alejandro Abuin , Joel Edwards , Charles Montgomery , Bobby Joe Payne , Carolina Rangel , Arthur T. Sands , Zheng-Zheng Shi , Mary Jean Sparks , Teresa Gail Townsend , Rosemary Girgis , Allison Anne Byers Horner , Peter Vogel , Brian Zambrowicz
发明人： Alejandro Abuin , Joel Edwards , Charles Montgomery , Bobby Joe Payne , Carolina Rangel , Arthur T. Sands , Zheng-Zheng Shi , Mary Jean Sparks , Teresa Gail Townsend , Rosemary Girgis , Allison Anne Byers Horner , Peter Vogel , Brian Zambrowicz
IPC分类号： A01K67/027 , C12N5/06 , C07K16/18
CPC分类号： A01K67/0276 , A01K2217/054 , A01K2217/075 , A01K2227/105 , A01K2267/0356 , C12N15/8509 , C12N2840/44
摘要： The current invention relates to genetically engineered mice, cells derived from those mice, and polynucleotides and polypeptides corresponding to genes affected by the engineered mutation. The invention also relates to antibodies raised in a mouse of the invention. The invention further provides methods for using the mice, cells, polynucleotides, polypeptides and antibodies of the invention.
摘要翻译： 本发明涉及基因工程小鼠，衍生自这些小鼠的细胞，以及对应于受工程突变影响的基因的多核苷酸和多肽。 本发明还涉及在本发明的小鼠中产生的抗体。 本发明还提供了使用本发明的小鼠，细胞，多核苷酸，多肽和抗体的方法。
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10.发明申请Genetically engineered and phenotyped mice and stem cell clones for producing the same 审中-公开基因工程和表型小鼠和干细胞克隆用于生产相同的公开(公告)号：US20080189799A1
公开(公告)日：2008-08-07
申请号：US11978234
申请日：2007-10-26
申请人： Liangfen Fan , Glenn Friedrich , Laurie Jeanette Minze , Charles Montgomery , Bobby Joe Payne , Carolina Rangel , Arthur T. Sands , Tracy Ellen Willis Sevaux , Zheng-Zheng Shi , Mary Jean Sparks , Peter Vogel , Brian Zambrowicz
发明人： Liangfen Fan , Glenn Friedrich , Laurie Jeanette Minze , Charles Montgomery , Bobby Joe Payne , Carolina Rangel , Arthur T. Sands , Tracy Ellen Willis Sevaux , Zheng-Zheng Shi , Mary Jean Sparks , Peter Vogel , Brian Zambrowicz
IPC分类号： C12N15/11 , A01K67/027
CPC分类号： A01K67/0276 , A01K2217/075 , A01K2267/03 , C07K14/705 , C07K14/70503 , C07K14/7151 , C07K14/775 , C07K14/8114 , C07K14/8121 , C12N9/1051 , C12N9/12 , C12N9/1205 , C12N2799/027 , C12N2800/60
摘要： The current invention relates to genetically engineered mice, cells derived from those mice, and polynucleotides and polypeptides corresponding to genes affected by the engineered mutation. The invention also relates to antibodies raised in a mouse of the invention. The invention further provides methods for using the mice, cells, polynucleotides, polypeptides and antibodies of the invention.
摘要翻译： 本发明涉及基因工程小鼠，衍生自这些小鼠的细胞，以及对应于受工程突变影响的基因的多核苷酸和多肽。 本发明还涉及在本发明的小鼠中产生的抗体。 本发明还提供了使用本发明的小鼠，细胞，多核苷酸，多肽和抗体的方法。
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