-
公开(公告)号：US5876945A
公开(公告)日：1999-03-02
申请号：US759581
申请日：1996-12-05
申请人： Dexter Allan Chisholm , Bruce Aaron Diner , Gail K. Donaldson , Howard Paul Hershey , Douglas Brian Jordan , Xiao Song Tang , Shaojie Wang , Jeffrey T. Trost , Patrick V. Warren
发明人： Dexter Allan Chisholm , Bruce Aaron Diner , Gail K. Donaldson , Howard Paul Hershey , Douglas Brian Jordan , Xiao Song Tang , Shaojie Wang , Jeffrey T. Trost , Patrick V. Warren
CPC分类号： C07H21/00 , C12Q1/37 , G01N33/535
摘要： D1 protease has been isolated from the alga (Scenedesmjus obliquus), wheat, and Synechocystis PCC 6803 and the genes encoding these enzymes have been cloned and sequenced. Native or recombinantly produced enzyme has been used to develop assays to detect herbicidal compositions capable of inhibiting the D1 protease enzyme.
-
公开(公告)号：US06350573B1
公开(公告)日：2002-02-26
申请号：US09304711
申请日：1999-05-04
IPC分类号： C12Q100
CPC分类号： C12Q1/37 , G01N2430/20 , G01N2500/00
摘要： D1 protease has been isolated from the alga (Scenedesmjus obliquus), wheat, and Synechocystis PCC 6803 and the genes encoding these enzymes have been cloned and sequenced. Native or recombinantly produced enzyme has been used to develop assays to detect herbicidal compositions capable of inhibiting the D1 protease enzyme.
摘要翻译： 已经从藻类（Scenedesmjus obliquus），小麦和集胞藻PCC 6803中分离出D1蛋白酶，并且已经克隆并测序了编码这些酶的基因。 已经使用天然或重组产生的酶开发测定以检测能够抑制D1蛋白酶的除草组合物。
-
公开(公告)号：US06444457B1
公开(公告)日：2002-09-03
申请号：US09173281
申请日：1998-10-15
申请人： Dexter Allan Chisholm , Bruce Aaron Diner , Gail K Donaldson , Howard Paul Hershey , Douglas Brian Jordan , Xiao Song Tang , Shaojie Wang
发明人： Dexter Allan Chisholm , Bruce Aaron Diner , Gail K Donaldson , Howard Paul Hershey , Douglas Brian Jordan , Xiao Song Tang , Shaojie Wang
IPC分类号： C12N950
CPC分类号： C07H21/00 , C12Q1/37 , G01N33/535
摘要： D1 protease has been isolated from the alga (Scenedesmjus obliquus), wheat, and Synechocystis PCC 6803 and the genes encoding these enzymes have been cloned and sequenced. Native or recombinantly produced enzyme has been used to develop assays to detect herbicidal compositions capable of inhibiting the D1 protease enzyme.
-
公开(公告)号：US6146866A
公开(公告)日：2000-11-14
申请号：US181183
申请日：1998-10-28
CPC分类号： C12N9/1085 , C12N15/8243 , C12N9/00
摘要： Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fingal RS in a transformed host cell. In vivo and in vitro methods to identify herbicide or fungicide candidates are included that evaluate the ability of a chemical compound to inhibit the activity of a plant or fungal LS enzyme or a plant or fungal RS enzyme.
摘要翻译： 通过大肠杆菌营养缺陷型功能互补，克隆了菠菜核黄素生物合成途径的最终和五倍体酶，即Lumazine合酶（LS）和核黄素合成酶（RS）。 本发明涉及从编码LS蛋白的植物或真菌中分离核酸片段。 本发明还涉及从编码RS蛋白的植物或真菌中分离核酸片段。 此外，本发明还涉及在正义或反义方向上构建编码LS的一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物LS水平的变化。 此外，本发明还涉及在正义或反义方向上编码RS的全部一部分的嵌合基因的构建，其中嵌合基因的表达导致在转化的宿主细胞中产生改变的植物或指甲的水平。 包括用于鉴定除草剂或杀真菌剂候选物的体内和体外方法，其评估化合物抑制植物或真菌LS酶或植物或真菌RS酶的活性的能力。
-
公开(公告)号：US07205331B2
公开(公告)日：2007-04-17
申请号：US09798423
申请日：2001-03-02
IPC分类号： A01N43/10 , A61K31/381 , C07D333/20
CPC分类号： A01N37/20 , A01N37/34 , A01N43/36 , A01N53/00 , C07C233/57 , C07C255/41 , C07C255/60 , C07D207/34 , C07D207/46 , C07D333/20 , C07D333/24 , C07D333/28 , C07D333/38
摘要： Compounds of Formula I are disclosed which are useful as fungicides wherein X is —O—, —CH(R11)—or ═C(R11)—; R1 is H or C1–C2 alkyl; R2 is H; C1–C6 alkyl; C3–C6 cycloalkyl; or phenyl optionally substituted with halogen, cyano, C1–C2 alkyl or C1–C2 alkoxy; and R3–R11 are as defined in the disclosure. Also disclosed are compositions containing the compounds of Formula I and a method for controlling plant diseases caused by fungal plant pathogens which involves applying an effective amount of a compound of Formula I.
摘要翻译： 公开了可用作杀真菌剂的式I化合物
-
公开(公告)号：US07135585B2
公开(公告)日：2006-11-14
申请号：US09798428
申请日：2001-03-02
IPC分类号： C07C255/03 , A01N37/34
CPC分类号： A01N37/20 , A01N37/34 , A01N43/36 , A01N53/00 , C07C233/57 , C07C255/41 , C07C255/60 , C07D207/34 , C07D207/46 , C07D333/20 , C07D333/24 , C07D333/28 , C07D333/38
摘要： Compounds of Formula I are disclosed which are useful as fungicides wherein Q is Z is X is —O—, —CH(R11)— or ═C(R11)—; R1 is H or C1–C2 alkyl; R2 is H; C1–C6 alkyl; C3–C6 cycloalkyl; or phenyl optionally substituted with halogen, cyano, C1–C2 alkyl or C1–C2 alkoxy; and R3–R11 are as defined in the disclosure. Also disclosed are compositions containing the compounds of Formula I and a method for controlling plant diseases caused by fungal plant pathogens which involves applying an effective amount of a compound of Formula I.
摘要翻译： 公开了可用作杀真菌剂的式I化合物，其中Q是Z是X是-O-，-CH（R 11）或-C（R 11） - ; R 1是H或C 1 -C 2烷基; R 2是H; C 1 -C 6烷基; C 1 -C 6烷基; C 3 -C 6环烷基; 或任选被卤素，氰基，C 1 -C 2烷基或C 1 -C 2烷氧基取代的苯基 ; R 3和R 3 - 如本公开所定义。 还公开了含有式I化合物的组合物和控制由真菌植物病原体引起的植物病害的方法，其涉及施用有效量的式I化合物。
-
7.发明授权Methods of identifying nucleic acid sequences encoding plant riboflavin synthase enzymes 失效鉴定编码植物核黄素合酶的核酸序列的方法公开(公告)号：US06682891B2
公开(公告)日：2004-01-27
申请号：US09874585
申请日：2001-06-05
IPC分类号： C12Q168
CPC分类号： C12N9/1085 , C12N9/00 , C12N15/8243
摘要： Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fungal RS in a transformed host cell. In vivo and in vitro methods to identify herbicide or fungicide candidates are included that evaluate the ability of a chemical compound to inhibit the activity of a plant or fungal LS enzyme or a plant or fungal RS enzyme.
摘要翻译： 通过大肠杆菌营养缺陷型功能互补，克隆了菠菜核黄素生物合成途径的最终和五倍体酶，即Lumazine合酶（LS）和核黄素合成酶（RS）。 本发明涉及从编码LS蛋白的植物或真菌中分离核酸片段。 本发明还涉及从编码RS蛋白的植物或真菌中分离核酸片段。 此外，本发明还涉及在正义或反义方向上构建编码LS的一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物LS水平的改变。 此外，本发明还涉及在正义或反义方向上构建编码RS的全部一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物或真菌RS水平的改变。 包括用于鉴定除草剂或杀真菌剂候选物的体内和体外方法，其评估化合物抑制植物或真菌LS酶或植物或真菌RS酶的活性的能力。
-
公开(公告)号：US06642392B1
公开(公告)日：2003-11-04
申请号：US09798421
申请日：2001-03-02
IPC分类号： C07D20730
CPC分类号： A01N37/20 , A01N37/34 , A01N43/36 , A01N53/00 , C07C233/57 , C07C255/41 , C07C255/60 , C07D207/34 , C07D207/46 , C07D333/20 , C07D333/24 , C07D333/28 , C07D333/38
摘要： Compounds of Formula I are disclosed which are useful as fungicides wherein X is —O—, —CH(R11)— or ═C(R11)—; R1 is H or C1-C2 alkyl; R2 is H; C1-C6 alkyl; C3-C6 cycloalkyl; or phenyl optionally substituted with halogen, cyano, C1-C2 alkyl or C1-C2 alkoxy; and R3-R11 are as defined in the disclosure. Also disclosed are compositions containing the compounds of Formula I and a method for controlling plant diseases caused by fungal plant pathogens which involves applying an effective amount of a compound of Formula I.
摘要翻译： 公开了式I化合物，其可用作杀真菌剂，其中X是-O-，-CH（R 11） - 或= C（R 11） - ; R 1是H或C 1 -C 2烷基; 2>是H; C1-C6烷基; C3-C6环烷基; 或任选被卤素，氰基，C 1 -C 2烷基或C 1 -C 2烷氧基取代的苯基; R 3 -R 11如本公开内容所定义。还公开了含有式I化合物和 控制真菌植物病原体引起的植物病害，其涉及施用有效量的式I化合物。
-
公开(公告)号：US06323013B1
公开(公告)日：2001-11-27
申请号：US09280040
申请日：1999-03-26
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N15/8243
摘要： Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fungal RS in a transformed host cell. In vivo and in vitro methods to identify herbicide or fungicide candidates are included that evaluate the ability of a chemical compound to inhibit the activity of a plant or fungal LS enzyme or a plant or fungal RS enzyme.
摘要翻译： 通过大肠杆菌营养缺陷型功能互补，克隆了菠菜核黄素生物合成途径的最终和五倍体酶，即Lumazine合酶（LS）和核黄素合成酶（RS）。 本发明涉及从编码LS蛋白的植物或真菌中分离核酸片段。 本发明还涉及从编码RS蛋白的植物或真菌中分离核酸片段。 此外，本发明还涉及在正义或反义方向上构建编码LS的一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物LS水平的变化。 此外，本发明还涉及在正义或反义方向上构建编码RS的全部一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物或真菌RS水平的改变。 包括用于鉴定除草剂或杀真菌剂候选物的体内和体外方法，其评估化合物抑制植物或真菌LS酶或植物或真菌RS酶的活性的能力。
-
公开(公告)号：US06350597B1
公开(公告)日：2002-02-26
申请号：US09277700
申请日：1999-03-26
IPC分类号： C12N900
CPC分类号： C12N9/1085 , C12N9/00 , C12N15/8243
摘要： Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fungal RS in a transformed host cell. In vivo and in vitro methods to identify herbicide or fungicide candidates are included that evaluate the ability of a chemical compound to inhibit the activity of a plant or fungal LS enzyme or a plant or fungal RS enzyme.
摘要翻译： 通过大肠杆菌营养缺陷型功能互补，克隆了菠菜核黄素生物合成途径的最终和五倍体酶，即Lumazine合酶（LS）和核黄素合成酶（RS）。 本发明涉及从编码LS蛋白的植物或真菌中分离核酸片段。 本发明还涉及从编码RS蛋白的植物或真菌中分离核酸片段。 此外，本发明还涉及在正义或反义方向上构建编码LS的一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物LS水平的变化。 此外，本发明还涉及在正义或反义方向上构建编码RS的全部一部分的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中植物或真菌RS水平的改变。 包括用于鉴定除草剂或杀真菌剂候选物的体内和体外方法，其评估化合物抑制植物或真菌LS酶或植物或真菌RS酶的活性的能力。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.028 秒)
