公开(公告)号：US20140030776A1

公开(公告)日：2014-01-30

申请号：US13838508

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： G06F19/22 ,  C12P7/16 ,  C12P7/40 ,  C12N9/88

CPC分类号： G06F19/22 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

公开(公告)号：US09611482B2

公开(公告)日：2017-04-04

申请号：US13837893

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Dennis Flint ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N15/81 ,  C12N9/88 ,  C07K14/395 ,  C12P7/16 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  G01N33/573

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

公开(公告)号：US09512435B2

公开(公告)日：2016-12-06

申请号：US13837921

申请日：2013-03-15

申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC

发明人： Dennis Flint ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N15/81 ,  C12N9/88 ,  C07K14/395 ,  C12P7/16 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  G01N33/573

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

公开(公告)号：US20140038268A1

公开(公告)日：2014-02-06

申请号：US13837893

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N15/81

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.

摘要翻译： 本发明涉及包含二羟基酸脱水酶多肽的重组宿主细胞，特别是酵母细胞。 本发明还涉及由于多肽的表达增加，细胞的Fe-S簇生物合成的调节或其组合而导致的具有增加的二羟基酸脱水酶多肽的比活性的重组宿主细胞。 本发明还包括使用宿主细胞的方法，以及用于鉴定增加宿主细胞中Fe-S簇生物合成途径中的通量的多肽的方法。

公开(公告)号：US08951937B2

公开(公告)日：2015-02-10

申请号：US13838508

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis Flint ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： C40B30/00 ,  C12P21/00 ,  G06F19/22 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12N1/21 ,  C12N1/19 ,  C12N9/88 ,  C12N15/00 ,  C12N5/10

CPC分类号： G06F19/22 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化成α-酮戊二酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。

公开(公告)号：US20140051137A1

公开(公告)日：2014-02-20

申请号：US13838570

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Steven Cary Rothman ,  Wonchul Suh ,  Jean-Francois Tomb ,  Rick W. Ye

IPC分类号： C12N9/88 ,  C12P7/16 ,  G06F19/22

CPC分类号： G16B30/00 ,  C12N9/88 ,  C12P7/16 ,  C12P7/40 ,  C12P13/06 ,  C12P13/08 ,  C12Y402/01009 ,  Y02E50/10

摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化为α-酮基甲基戊酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。

公开(公告)号：US20140038263A1

公开(公告)日：2014-02-06

申请号：US13837921

申请日：2013-03-15

申请人： Butamax(TM) Advanced Biofuels LLC

发明人： Dennis FLINT ,  Brian James Paul ,  Rick W. Ye

IPC分类号： C12N9/88

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N9/88 ,  C12N15/81 ,  C12P7/40 ,  C12P13/02 ,  C12P13/04 ,  C12Q1/527 ,  C12Y402/01009 ,  G01N33/573 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.