公开(公告)号：US20140147554A1

公开(公告)日：2014-05-29

申请号：US14166643

申请日：2014-01-28

申请人： CHINA NATIONAL RESEARCH INSTITUTE OF FOOD AND FERMENTATION INDUSTRIES

发明人： Muyi Cai ,  Ruizeng Gu ,  Jun Lu ,  Weixue Yi ,  Zhe Dong ,  Yong Ma ,  Xingchang Pan ,  Yaguang Xu ,  Yongqing Ma ,  Feng Lin ,  Zhentao Jin ,  Wenying Liu ,  Liang Chen ,  Lu Lu

IPC分类号： A23L2/02 ,  A23L2/66

CPC分类号： A23L2/02 ,  A23L2/382 ,  A23L2/60 ,  A23L2/66 ,  A23L33/135 ,  A23L33/14 ,  A23L33/18 ,  A23L33/21 ,  A23V2002/00 ,  A23V2200/318 ,  A23V2200/3202 ,  A23V2200/3204 ,  A23V2250/042 ,  A23V2250/284 ,  A23V2250/5422 ,  A23V2250/76

摘要： The present invention discloses an enzyme beverage of natural garden stuff and a method for preparing the same. The enzyme beverage is prepared from a fresh juice extracted from fresh garden stuffs and added with marine fish-skin collagen peptide, as a fermentation substrate, by dilution, blending and sterilization followed by stepwise fermentation with lactobacillus and yeast in sequence. With stepwise fermentation of the garden stuff juice from two or more fresh garden stuffs with lactobacillus and yeast, and the addition of marine fish-skin collagen peptide for accelerating growth and metabolism of fermenting strains, said beverage contains more abundant metabolites from the nutritional ingredients of the natural garden stuffs, and will bring more beneficial and nutritional effects on health.

摘要翻译： 本发明公开了一种天然花园材料的酶饮料及其制备方法。 该酶饮料由新鲜花园材料提取的新鲜果汁制成，并通过稀释，混合和灭菌，然后依次用乳杆菌和酵母进行逐步发酵，作为发酵底物，加入海洋鱼皮胶原肽。 从两个或更多新鲜的园艺产品中，用乳杆菌和酵母逐步发酵花生酱汁，并加入海洋鱼皮胶原蛋白肽以加速发酵菌株的生长和代谢，所述饮料含有更丰富的营养成分的代谢物 天然花园的东西，并将为健康带来更多的有益和营养的影响。

公开(公告)号：US09974321B2

公开(公告)日：2018-05-22

申请号：US15442574

申请日：2017-02-24

申请人： CHINA NATIONAL RESEARCH INSTITUTE OF FOOD AND FERMENTATION INDUSTRIES

发明人： Muyi Cai ,  Ruizeng Gu ,  Jun Lu ,  Tao Ma ,  Xingchang Pan ,  Zhe Dong ,  Yong Ma ,  Yaguang Xu ,  Yongqing Ma ,  Zhentao Jin ,  Liang Chen ,  Lu Lu ,  Wenying Liu ,  Ying Wei ,  Haixin Zhang ,  Yan Liu ,  Kelu Cao ,  Jing Wang ,  Guoming Li ,  Ming Zhou

IPC分类号： A23J1/04 ,  A23J3/04 ,  A23J3/34 ,  A23L33/17 ,  A23L17/20 ,  C07K14/46 ,  A23L33/18

CPC分类号： A23J1/04 ,  A23J3/04 ,  A23J3/34 ,  A23J3/341 ,  A23L17/20 ,  A23L33/17 ,  A23L33/18 ,  A23V2002/00 ,  C07K14/461 ,  A23V2200/304 ,  A23V2250/542 ,  A23V2250/55

摘要： Disclosed are a fish protein oligopeptide with low allergenicity and slight fishiness, and industrial preparation method and application thereof. The method comprises the following steps: 1) washing fresh fish flesh and/or fish wastes, crushing, and adding water to obtain a mixture; 2) performing thermal denaturation on the mixture to obtain a denaturized protein solution; 3) centrifuging the denaturized protein solution to obtain a precipitate, and adding water into the precipitate and grinding, to obtain a slurry; 4) adjusting the slurry to pH 6-9, and sequentially adding a neutral protease, a papain and an alkaline protease to conduct enzymolysis, and after enzyme inactivation, to obtain an enzymatic hydrolysate; 5) centrifuging the enzymatic hydrolysate, and performing membrane filtration on centrifuged supernatant, to obtain the fish protein oligopeptide with low allergenicity and slight fishiness. The method completely eliminates the allergenicity and fishiness of fish proteins, and prevents release of bitter components.

公开(公告)号：US09609883B2

公开(公告)日：2017-04-04

申请号：US14515457

申请日：2014-10-15

申请人： CHINA NATIONAL RESEARCH INSTITUTE OF FOOD AND FERMENTATION INDUSTRIES

发明人： Muyi Cai ,  Ruizeng Gu ,  Jun Lu ,  Feng Lin ,  Yong Ma ,  Zhe Dong ,  Xingchang Pan ,  Yongqing Ma ,  Yaguang Xu ,  Zhentao Jin ,  Liang Chen ,  Wenying Liu ,  Ying Wei ,  Haixin Zhang ,  Lu Lu ,  Yan Liu ,  Tao Ma ,  Simeng Jiang ,  Kelu Cao ,  Jing Wang

IPC分类号： C12P1/00 ,  A23J3/34 ,  A23J3/18 ,  C12P21/06 ,  C07K5/093 ,  C07K4/10 ,  A23L33/18 ,  A61K38/00

CPC分类号： A23J3/346 ,  A23J3/18 ,  A23L33/18 ,  A61K38/00 ,  C07K4/10 ,  C07K5/0819 ,  C12P21/06

摘要： The present invention discloses a method for producing wheat glutamine peptide using wheat gluten powder as raw material, belonging to the fields of food and biotechnology. The method includes the steps of: performing enzymolysis in two steps using Alcalase and papain with the wheat gluten powder as raw material, to obtain the wheat glutamine peptide with components with molecular weight of less than 1000 Da being more than 90%, characteristic glutamine peptide segment glutamine-arginine-glutamine (Gln-Arg-Gln, QRQ) content being more than 2.0% and glutamine content being up to 23.54% by treating the enzymatic hydrolysate by centrifugation, ultrafiltration, concentration, spray drying, etc. The produced glutamine peptide can be used as functional nutrition composition ingredient in the development and production of ordinary foods, health foods and medicines.

公开(公告)号：US09534026B2

公开(公告)日：2017-01-03

申请号：US14583058

申请日：2014-12-24

申请人： CHINA NATIONAL RESEARCH INSTITUTE OF FOOD AND FERMENTATION INDUSTRIES

发明人： Muyi Cai ,  Feng Lin ,  Yan Liu ,  Haixin Zhang ,  Ruizeng Gu ,  Jun Lu ,  Ying Wei

IPC分类号： C12N5/00 ,  C07K14/415 ,  C12P21/06

CPC分类号： C07K14/415 ,  C12N5/0043 ,  C12N2500/76 ,  C12P21/06 ,  C12Y304/00 ,  Y02P20/52

摘要： The present invention provides a corn active peptide additive for cell culture medium, wherein in the corn active peptide additive, oligopeptides with molecular weight of lower than 1000 Dalton account for equal to or more than 90 wt % of total proteins, and the oligopeptides at least comprise one or more of AP, SAP, PAL, VNAP, PSSQ, and TQPGPQ. The corn active peptide additive of the present invention can be compounded with various basic culture mediums for serum-free culture of various animal cells, which not only substantially lowers the cost for cell culturing and reduces pollution and other problems caused by an animal derived component, but also can promote cell proliferation, improve cell viability and enhance expression of cell products.

摘要翻译： 本发明提供了一种用于细胞培养基的玉米活性肽添加剂，其中在玉米活性肽添加剂中，分子量低于1000道尔顿的寡肽占总蛋白质的90重量％以上，寡肽至少 包括AP，SAP，PAL，VNAP，PSSQ和TQPGPQ中的一个或多个。 本发明的玉米活性肽添加剂可以与各种碱性培养基混合，用于各种动物细胞的无血清培养，不仅大大降低了细胞培养的成本，还减少了由动物衍生成分引起的污染和其它问题， 而且可以促进细胞增殖，提高细胞活力，增强细胞产物的表达。

公开(公告)号：US08940685B2

公开(公告)日：2015-01-27

申请号：US13894379

申请日：2013-05-14

申请人： China National Research Institute of Food and Fermentation Industries

发明人： Muyi Cai ,  Ruizeng Gu ,  Weixue Yi ,  Jun Lu ,  Yong Ma ,  Zhe Dong ,  Yaguang Xu ,  Xingchang Pan ,  Yongqing Ma ,  Feng Lin ,  Zhentao Jin ,  Liang Chen ,  Lu Lu ,  Wenying Liu

IPC分类号： C12P21/06 ,  A61K36/899 ,  A61K36/88 ,  C07K14/415 ,  C07K5/062 ,  A61K38/01

CPC分类号： C12P21/06 ,  A61K36/899 ,  A61K38/011 ,  C07K5/06026 ,  C07K14/415

摘要： The present invention discloses a method for producing antihypertensive active peptides with corn germ protein as the material. The method comprises an alkali-heat treatment and continuous enzymolysis of the corn germ protein. The components with molecular weight less than 1000 Da in the active peptides obtained according to the present method account for more than 92%, and alanine-tyrosine (Ala-Tyr, AY) as the characteristic peptide fragments in the antihypertensive peptides accounts for more than 0.6%, so that the active peptides have a good ACE inhibitory activity in vitro as well as stability against temperature, pH and major gastrointestinal digestive enzymes, and have a significant effect of lowering blood pressure on spontaneous hypertension rats in vivo. The active peptides can be applied as a new functional nutrient to development and production of food, health food and pharmaceutical.

摘要翻译： 本发明公开了以玉米胚芽蛋白为材料制备抗高血压活性肽的方法。 该方法包括玉米胚芽蛋白的碱热处理和连续酶解。 在根据本方法获得的活性肽中分子量小于1000Da的组分占92％以上，作为抗高血压肽中特征性肽片段的丙氨酸 - 酪氨酸（Ala-Tyr，AY）占比超过 0.6％，使得活性肽在体外具有良好的ACE抑制活性以及对温度，pH和主要胃肠消化酶的稳定性，并且对体内自发性高血压大鼠具有降低血压的显着作用。 活性肽可以作为新的功能营养物来开发和生产食品，保健食品和药物。