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公开(公告)号：US20210261939A1
公开(公告)日：2021-08-26
申请号：US16763041
申请日：2018-11-15
发明人： Su Jin Kim , Young Mi Lee , Yang Hee Kim , Seong Bo Kim , Seung Won Park , Eun Jung Choi
摘要： The present disclosure relates to novel D-psicose 3-epimerase and a method for producing psicose using the same.
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公开(公告)号：US11028420B2
公开(公告)日：2021-06-08
申请号：US16624973
申请日：2018-06-22
发明人： Min Hoe Kim , Joo Hang Lee , Hye Jin Jo , Seong Bo Kim , Yang Hee Kim , Jung Eun Kim , Seung Won Park , Eun Jung Choi
摘要： The present application relates to a method for producing D-psicose, the method comprising the steps of: putting a composition containing a D-psicose borate complex into a chromatography comprising divalent cations; and separating the composition containing the D-psicose borate complex into a D-psicose-containing fraction (i) and a borate-containing fraction (ii).
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公开(公告)号：US10544438B2
公开(公告)日：2020-01-28
申请号：US15521243
申请日：2015-10-19
发明人： Yang Hee Kim , Sung Jae Yang , Young Mi Lee , Il Hyang Park , Seong Bo Kim , Hyun Kug Cho , Seung Won Park
摘要： The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.
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公开(公告)号：US09896705B2
公开(公告)日：2018-02-20
申请号：US15413960
申请日：2017-01-24
发明人： In Seok Oh , Chang Gyeom Kim , Seung Hyun Cho , Seong Bo Kim , Yang Hee Kim , Kyong Yeon Cho , Sung Jae Yang , Jin Ha Kim
摘要： The present invention relates to the development of an L-arabinose isomerase variant from Thermotoga neapolitana DSM 5068, which is a kind of thermophile, on the basis of protein molecular modeling. Moreover, the present invention relates to a method of producing D-tagatose from D-galactose by using the enzyme or a microorganism of the genus Corynebacterium expressing the enzyme.
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5.发明申请METHOD FOR STERILIZING MICROBIAL CELLS USING POLYETHYLENE GLYCOL-BASED NONIONIC SURFACTANT 有权使用聚乙烯甘油基非离子表面活性剂灭菌微生物细胞的方法公开(公告)号：US20140302564A1
公开(公告)日：2014-10-09
申请号：US14311185
申请日：2014-06-20
发明人： Jin Ha Kim , Yong Mi Lee , Seong Bo Kim , Taek Beom Kim , Yang Hee Kim , Seung Won Park
IPC分类号： A23L1/03 , A23L3/3508
CPC分类号： A23L29/06 , A23L3/3508 , A23L29/065 , C12N1/20 , C12N9/90 , C12N11/16 , C12P19/02 , C12P19/24 , C12Y503/01003 , Y02P20/52
摘要： A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.
摘要翻译： 提供了一种灭菌微生物细胞的方法。 根据该方法，用聚乙二醇类非离子表面活性剂处理微生物细胞或含有微生物细胞的培养物，使得几乎所有的微生物细胞在微生物细胞中表达的酶活性保持在高水平的同时进行灭菌。 将微生物细胞灭菌的方法和含有灭菌的微生物细胞的材料，其中使用聚乙二醇类非离子表面活性剂将微生物细胞灭菌，可以用于食品，使得将微生物细胞灭菌以用于食品生产。 此外，含有灭菌的微生物细胞的材料可用于制备塔格糖的方法，其中产生半乳糖和/或阿拉伯糖异构酶的棒状杆菌属微生物细胞使用聚乙二醇基非离子表面活性剂灭菌。
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公开(公告)号：US11499147B2
公开(公告)日：2022-11-15
申请号：US17076728
申请日：2020-10-21
发明人： Sung Jae Yang , Young Mi Lee , Il Hyang Park , Chan-Hyoung Lee , Hyun Kug Cho , Seong Bo Kim , Yang Hee Kim , Seung Won Park
摘要： Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
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公开(公告)号：US10947524B2
公开(公告)日：2021-03-16
申请号：US16060018
申请日：2017-07-31
发明人： Sung Jae Yang , Young Mi Lee , Il Hyang Park , Chan-Hyoung Lee , Hyun Kug Cho , Seong Bo Kim , Yang Hee Kim , Seung Won Park
摘要： Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
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公开(公告)号：US10550414B2
公开(公告)日：2020-02-04
申请号：US16065749
申请日：2016-12-23
发明人： Jung Eun Kim , Yang Hee Kim , Seong Bo Kim , Seung Won Park , Ji Hyun Kang
摘要： The present application relates to a composition for producing D-psicose comprising (a) a D-psicose 3-epimerase, a strain expressing the enzyme or a culture of the strain, and (b) at least one salt selected from the group consisting of an aluminate and an iodate, and a method for producing D-psicose from D-fructose or a method for increasing the conversion of D-fructose into D-psicose comprising adding (b) to (a).
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公开(公告)号：US10544439B2
公开(公告)日：2020-01-28
申请号：US15759511
申请日：2017-07-31
发明人： Sung Jae Yang , Young Mi Lee , Il Hyang Park , Chan-Hyoung Lee , Hyun Kug Cho , Seong Bo Kim , Yang Hee Kim , Seung Won Park
摘要： Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
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10.发明授权Method for sterilizing microbial cells using polyethylene glycol-based nonionic surfactant 有权使用聚乙二醇类非离子表面活性剂灭菌微生物细胞的方法公开(公告)号：US09504271B2
公开(公告)日：2016-11-29
申请号：US14311185
申请日：2014-06-20
发明人： Jin Ha Kim , Young Mi Lee , Seong Bo Kim , Taek Beom Kim , Yang Hee Kim , Seung Won Park
CPC分类号： A23L29/06 , A23L3/3508 , A23L29/065 , C12N1/20 , C12N9/90 , C12N11/16 , C12P19/02 , C12P19/24 , C12Y503/01003 , Y02P20/52
摘要： A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.
摘要翻译： 提供了一种灭菌微生物细胞的方法。 根据该方法，用聚乙二醇类非离子表面活性剂处理微生物细胞或含有微生物细胞的培养物，使得几乎所有的微生物细胞在微生物细胞中表达的酶活性保持在高水平的同时进行灭菌。 将微生物细胞灭菌的方法和含有灭菌的微生物细胞的材料，其中使用聚乙二醇类非离子表面活性剂将微生物细胞灭菌，可以用于食品，使得将微生物细胞灭菌以用于食品生产。 此外，含有灭菌的微生物细胞的材料可用于制备塔格糖的方法，其中产生半乳糖和/或阿拉伯糖异构酶的棒状杆菌属微生物细胞使用聚乙二醇基非离子表面活性剂灭菌。
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