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    METHOD FOR STERILIZING MICROBIAL CELLS USING POLYETHYLENE GLYCOL-BASED NONIONIC SURFACTANT
    3.
    发明申请
    METHOD FOR STERILIZING MICROBIAL CELLS USING POLYETHYLENE GLYCOL-BASED NONIONIC SURFACTANT 有权
    使用聚乙烯甘油基非离子表面活性剂灭菌微生物细胞的方法

    公开(公告)号：US20140302564A1

    公开(公告)日：2014-10-09

    申请号：US14311185

    申请日：2014-06-20

    Applicant: CJ CHEILJEDANG CORPORATION

    Inventor： Jin Ha Kim ,  Yong Mi Lee ,  Seong Bo Kim ,  Taek Beom Kim ,  Yang Hee Kim ,  Seung Won Park

    IPC: A23L1/03 ,  A23L3/3508

    CPC classification number: A23L29/06 ,  A23L3/3508 ,  A23L29/065 ,  C12N1/20 ,  C12N9/90 ,  C12N11/16 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01003 ,  Y02P20/52

    Abstract: A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.

    Abstract translation: 提供了一种灭菌微生物细胞的方法。 根据该方法，用聚乙二醇类非离子表面活性剂处理微生物细胞或含有微生物细胞的培养物，使得几乎所有的微生物细胞在微生物细胞中表达的酶活性保持在高水平的同时进行灭菌。 将微生物细胞灭菌的方法和含有灭菌的微生物细胞的材料，其中使用聚乙二醇类非离子表面活性剂将微生物细胞灭菌，可以用于食品，使得将微生物细胞灭菌以用于食品生产。 此外，含有灭菌的微生物细胞的材料可用于制备塔格糖的方法，其中产生半乳糖和/或阿拉伯糖异构酶的棒状杆菌属微生物细胞使用聚乙二醇基非离子表面活性剂灭菌。

    APPARATUS FOR PREPARING ENZYME-IMMOBILIZED BEADS AND METHOD FOR PREPARING ENZYME-IMMOBILIZED BEADS USING SAME
    5.
    发明申请
    APPARATUS FOR PREPARING ENZYME-IMMOBILIZED BEADS AND METHOD FOR PREPARING ENZYME-IMMOBILIZED BEADS USING SAME 有权
    用于制备酶固定的珠的装置和使用其制备酶固定的珠的方法

    公开(公告)号：US20150125922A1

    公开(公告)日：2015-05-07

    申请号：US14401780

    申请日：2013-05-10

    Applicant: CJ CHEILJEDANG CORPORATION

    Inventor： Soon Chul Kim ,  Seong Bo Kim ,  Jae Youn Lim ,  Kwang Jin An ,  Jin Ha Kim

    IPC: C12N11/00

    CPC classification number: C12N11/00 ,  C12M21/14 ,  C12M25/16

    Abstract: The present invention relates to an apparatus for preparing enzyme-immobilized beads used for preparation of tagatose, and a method for preparing enzyme-immobilized beads using the same. More specifically, the present invention relates to an apparatus for preparing enzyme-immobilized beads, comprising a nozzle with an inside diameter of 0.1-1 mm having a cylindrical lower end and comprising a cut-type liquid outlet (cut perpendicularly to the vertical axis of the nozzle) formed at the lower end, and a method for preparing enzyme-immobilized beads using the same.

    Abstract translation: 本发明涉及一种用于制备塔格糖的制备酶固定化珠粒的装置，以及使用其制备固定化酶的珠粒的方法。 更具体地说，本发明涉及一种用于制备酶固定珠粒的装置，其包括内径为0.1-1mm的喷嘴，其具有圆柱形下端，并且包括切割液体出口（垂直于垂直于 喷嘴），以及使用其制备固定化酶的珠粒的方法。

    Method for sterilizing microbial cells using polyethylene glycol-based nonionic surfactant
    8.
    发明授权
    Method for sterilizing microbial cells using polyethylene glycol-based nonionic surfactant 有权
    使用聚乙二醇类非离子表面活性剂灭菌微生物细胞的方法

    公开(公告)号：US09504271B2

    公开(公告)日：2016-11-29

    申请号：US14311185

    申请日：2014-06-20

    Applicant: CJ CHEILJEDANG CORPORATION

    Inventor： Jin Ha Kim ,  Young Mi Lee ,  Seong Bo Kim ,  Taek Beom Kim ,  Yang Hee Kim ,  Seung Won Park

    IPC: A23L1/03 ,  C12N1/20 ,  C12N9/90 ,  C12N11/16 ,  A23L3/3508 ,  C12P19/02 ,  C12P19/24

    CPC classification number: A23L29/06 ,  A23L3/3508 ,  A23L29/065 ,  C12N1/20 ,  C12N9/90 ,  C12N11/16 ,  C12P19/02 ,  C12P19/24 ,  C12Y503/01003 ,  Y02P20/52

    Abstract: A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.

    Abstract translation: 提供了一种灭菌微生物细胞的方法。 根据该方法，用聚乙二醇类非离子表面活性剂处理微生物细胞或含有微生物细胞的培养物，使得几乎所有的微生物细胞在微生物细胞中表达的酶活性保持在高水平的同时进行灭菌。 将微生物细胞灭菌的方法和含有灭菌的微生物细胞的材料，其中使用聚乙二醇类非离子表面活性剂将微生物细胞灭菌，可以用于食品，使得将微生物细胞灭菌以用于食品生产。 此外，含有灭菌的微生物细胞的材料可用于制备塔格糖的方法，其中产生半乳糖和/或阿拉伯糖异构酶的棒状杆菌属微生物细胞使用聚乙二醇基非离子表面活性剂灭菌。

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