公开(公告)号：US20110118442A1

公开(公告)日：2011-05-19

申请号：US12984132

申请日：2011-01-04

申请人： CARINA ENGSTRAND ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

发明人： CARINA ENGSTRAND ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

IPC分类号： C07K1/16 ,  C07C211/00 ,  A61L2/00 ,  B01D15/08

CPC分类号： B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

公开(公告)号：US07714112B2

公开(公告)日：2010-05-11

申请号：US11577203

申请日：2005-10-21

申请人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

发明人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

IPC分类号： C07K16/00 ,  C07K1/22

CPC分类号： B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

摘要： The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface.

摘要翻译： 本发明涉及从液体样品中的其它化合物分离抗体的方法，其中包含所述样品的流动相与多模式分离基质接触以吸附不期望的化合物，同时抗体在液体中保持游离， 其中所述多模式分离基质包含能够与所述目标化合物的带负电荷位点相互作用的第一组和能够与所述目标化合物的电荷 - 电荷相互作用以外的至少一个相互作用的第二组。 本发明还涉及一种填充有上述多模式分离基质的色谱柱，以及具有吸附在其表面上的多模式基团的过滤器。

公开(公告)号：US08895710B2

公开(公告)日：2014-11-25

申请号：US12984132

申请日：2011-01-04

申请人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

发明人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

IPC分类号： C07K1/14 ,  B01D15/36 ,  B01D15/38 ,  B01J20/286 ,  B01J41/20 ,  B01J20/32 ,  B01D15/32 ,  C07K16/06 ,  C07K1/36

CPC分类号： B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

公开(公告)号：US07867784B2

公开(公告)日：2011-01-11

申请号：US11577208

申请日：2005-10-21

申请人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

发明人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J. Johansson ,  Jean-Luc Maloisel

IPC分类号： G01N33/544 ,  G01N33/548 ,  G01N33/551 ,  C12M1/34 ,  C07C211/44

CPC分类号： B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

摘要： The present invention relates to a chromatography ligand defined by the following formula (I) R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式（I）R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

公开(公告)号：US20070259453A1

公开(公告)日：2007-11-08

申请号：US11577203

申请日：2005-10-21

申请人： Carina Engstrand ,  Annika Forss ,  Bo-Lennart Johansson ,  Hans Johansson ,  Jean-Luc Maloisel ,  Gunnar Glad

发明人： Carina Engstrand ,  Annika Forss ,  Bo-Lennart Johansson ,  Hans Johansson ,  Jean-Luc Maloisel ,  Gunnar Glad

IPC分类号： G01N33/53

CPC分类号： B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

摘要： The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface

摘要翻译： 本发明涉及从液体样品中的其它化合物分离抗体的方法，其中包含所述样品的流动相与多模式分离基质接触以吸附不需要的化合物，同时抗体在液体中保持游离， 其中所述多模式分离基质包含能够与所述目标化合物的带负电荷位点相互作用的第一组和能够与所述目标化合物的电荷 - 电荷相互作用以外的至少一个相互作用的第二组。 本发明还涉及一种填充有上述多模式分离基质的色谱柱和一种具有吸附在其表面上的多模式基团的过滤器

公开(公告)号：US20070244307A1

公开(公告)日：2007-10-18

申请号：US11577208

申请日：2005-10-21

申请人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans Johansson ,  Jean-Luc Maloisel

发明人： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans Johansson ,  Jean-Luc Maloisel

IPC分类号： C07K1/16 ,  C07C211/03 ,  G01N30/02

CPC分类号： B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

摘要： The present invention relates to a chromatography ligand defined by the following formula (I) R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式（I）定义的色谱配体R 1 -R 2 -N（R 3 N）-R 其中R 1是取代或未取代的苯基;其中R 1是取代或未取代的苯基; R 2是包含0-4个碳原子的烃链; R 3是包含1-3个碳原子的烃链; R 4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜偶联的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。