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11 条记录 (耗时 0.032 秒)

    Acidophilic Enzymes
    4.
    发明申请
    Acidophilic Enzymes 失效
    嗜酸性酶

    公开(公告)号：US20100196965A1

    公开(公告)日：2010-08-05

    申请号：US11885109

    申请日：2006-03-03

    申请人： Olga Golyshina ,  Peter Golyshin ,  Kenneth Timmis ,  Manuel Ferrer

    发明人： Olga Golyshina ,  Peter Golyshin ,  Kenneth Timmis ,  Manuel Ferrer

    IPC分类号： C12P19/34 ,  C12N9/00 ,  C12N9/16 ,  C12N9/24

    CPC分类号： C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/93

    摘要： The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium Ferroplasma acidiphilum. In general, the present invention provides enzymes which are active and stable at acidic pH values, especially at pH values from 1 to 4, especially in the range of pH 2 to 3, obtainable from Ferroplasma acidiphilum, especially to an esterase, glycosidases and a DNA ligase. In addition to stability and activity at low pH values, the enzymes according to the present invention are all dependent on Fe2+ for their catalytic activity.

    摘要翻译： 本发明涉及在pH低于5.0时具有催化活性的酶。 本发明提供了可从古细菌获得的水解酶，详细地可从碱性阿斯法氏球菌获得的水解酶。 通常，本发明提供了在酸性pH值下，特别是在pH值为1至4，特别是在pH2至3的pH值范围内具有活性和稳定性的酶，其可从碳酸亚铁（Ferroplasma acidiphilum），特别是酯酶，糖苷酶和 DNA连接酶。 除了在低pH值下的稳定性和活性之外，根据本发明的酶全部依赖于Fe 2+作为其催化活性。

    Acidophilic enzymes
    5.
    发明授权
    Acidophilic enzymes 失效
    嗜酸酶

    公开(公告)号：US08003357B2

    公开(公告)日：2011-08-23

    申请号：US11885109

    申请日：2006-03-03

    申请人： Olga Golyshina ,  Peter Golyshin ,  Kenneth Timmis ,  Manuel Ferrer

    发明人： Olga Golyshina ,  Peter Golyshin ,  Kenneth Timmis ,  Manuel Ferrer

    IPC分类号： C12N9/00 ,  C12P19/34 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/93

    摘要： The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium Ferroplasma acidiphilum. In general, the present invention provides enzymes which are active and stable at acidic pH values, especially at pH values from 1 to 4, especially in the range of pH 2 to 3, obtainable from Ferroplasma acidiphilum, especially to an esterase, glycosidases and a DNA ligase. In addition to stability and activity at low pH values, the enzymes according to the present invention are all dependent on Fe2+ for their catalytic activity.

    摘要翻译： 本发明涉及在pH低于5.0时具有催化活性的酶。 本发明提供了可从古细菌获得的水解酶，详细地可从碱性阿斯法氏球菌获得的水解酶。 通常，本发明提供了在酸性pH值下，特别是在pH值为1至4，特别是在pH2至3的pH值范围内具有活性和稳定性的酶，其可从碳酸亚铁（Ferroplasma acidiphilum），特别是酯酶，糖苷酶和 DNA连接酶。 除了在低pH值下的稳定性和活性之外，根据本发明的酶全部依赖于Fe 2+作为其催化活性。

    Transgenic organisms with lower growth temperature
    8.
    发明申请
    Transgenic organisms with lower growth temperature 有权
    生长温度较低的转基因生物

    公开(公告)号：US20090255014A1

    公开(公告)日：2009-10-08

    申请号：US10575505

    申请日：2004-10-11

    申请人： Manuel Ferrer ,  Kenneth Timmis ,  Tatjana Chernikova ,  Peter Golyshin ,  Michail Yakimov

    发明人： Manuel Ferrer ,  Kenneth Timmis ,  Tatjana Chernikova ,  Peter Golyshin ,  Michail Yakimov

    IPC分类号： A01H5/00 ,  C12P21/00 ,  C12P21/02

    CPC分类号： C12R1/01 ,  C12N1/20

    摘要： The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.

    摘要翻译： 本发明涉及生物体，特别是植物和微生物的生长温度以及可耐受栽培温度的操作。 更具体地，本发明涉及异源蛋白在微生物中的表达，特别涉及在细菌中革兰氏阴性或革兰氏阳性的热敏蛋白的异源表达。 在第一方面，本发明提供了一种用于操作细胞和所得细胞的方法，其中表达来自编码至少一个伴侣或伴侣蛋白的嗜热微生物的至少一个基因。 这样的细胞选自培养的真核细胞，即动物和植物细胞以及整个植物，革兰氏阴性和革兰氏阳性细菌，真菌和酵母。

    Transgenic organisms with lower growth temperature
    10.
    发明授权
    Transgenic organisms with lower growth temperature 有权
    生长温度较低的转基因生物

    公开(公告)号：US07811784B2

    公开(公告)日：2010-10-12

    申请号：US10575505

    申请日：2004-10-11

    申请人： Manuel Ferrer ,  Kenneth Timmis ,  Tatjana Chernikova ,  Peter Golyshin ,  Michail Yakimov

    发明人： Manuel Ferrer ,  Kenneth Timmis ,  Tatjana Chernikova ,  Peter Golyshin ,  Michail Yakimov

    IPC分类号： C12P21/06 ,  C12N9/00

    CPC分类号： C12R1/01 ,  C12N1/20

    摘要： The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.

    摘要翻译： 本发明涉及生物体，特别是植物和微生物的生长温度以及可耐受栽培温度的操作。 更具体地，本发明涉及异源蛋白在微生物中的表达，特别涉及在细菌中革兰氏阴性或革兰氏阳性的热敏蛋白的异源表达。 在第一方面，本发明提供了一种用于操作细胞和所得细胞的方法，其中表达来自编码至少一个伴侣或伴侣蛋白的嗜热微生物的至少一个基因。 这样的细胞选自培养的真核细胞，即动物和植物细胞以及整个植物，革兰氏阴性和革兰氏阳性细菌，真菌和酵母。