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11 条记录 (耗时 0.022 秒)

    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex
    1.
    发明申请
    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex 审中-公开
    使用固定化金属 - 配体复合物纯化核酸的方法和设备

    公开(公告)号：US20060275815A1

    公开(公告)日：2006-12-07

    申请号：US11446795

    申请日：2006-06-05

    申请人： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    发明人： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C12N15/1006

    摘要： Provided is a method of purifying nucleic acids using a metal-ligand complex, the method comprising: immobilizing the metal-ligand complex on a solid support; bringing a sample containing the nucleic acid into contact with the immobilized complex on the solid support to bind the nucleic acid to the complex; and adding a solution containing a chelate capable of removing the metal to elute the nucleic acid bound to the complex. The nucleic acids can be efficiently purified using a metal-ligand complex interacting with a base of the nucleic acid instead of interacting with a phosphoric acid of the nucleic acid to selectively bind the nucleic acid, and using a chelator capable of removing the metal to elute the nucleic acid.

    摘要翻译： 提供了使用金属 - 配体络合物纯化核酸的方法，所述方法包括：将所述金属 - 配体复合物固定在固体支持物上; 使含有核酸的样品与固体支持物上的固定化复合物接触以将核酸结合到复合物上; 并加入含有能除去金属的螯合物的溶液以洗脱与络合物结合的核酸。 可以使用与核酸的碱基相互作用的金属 - 配体复合物来有效纯化核酸，而不是与核酸的磷酸相互作用以选择性地结合核酸，并且使用能够去除金属洗脱的螯合剂 核酸。

    Nucleic acid purification apparatus including photovoltaic device, microfluidic apparatus including the nucleic acid purification apparatus, and method of purifying nucleic acid using the nucleic acid purification apparatus
    4.
    发明申请
    Nucleic acid purification apparatus including photovoltaic device, microfluidic apparatus including the nucleic acid purification apparatus, and method of purifying nucleic acid using the nucleic acid purification apparatus 审中-公开
    包含光电装置的核酸纯化装置，包括核酸纯化装置的微流体装置和使用核酸纯化装置纯化核酸的方法

    公开(公告)号：US20070042396A1

    公开(公告)日：2007-02-22

    申请号：US11347407

    申请日：2006-02-03

    申请人： Jong-myeon Park ,  Sang-hyun Peak

    发明人： Jong-myeon Park ,  Sang-hyun Peak

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12N15/1003 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/1805 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0487

    摘要： Provided are a nucleic acid purification apparatus including a chamber that includes an inlet and an outlet, at least two electrodes that defines the chamber and form an electric field, a photovoltaic device that applies a bias to the electrodes, a microfluidic apparatus including the same, and a method of purifying a nucleic acid using the nucleic acid purification apparatus. The nucleic acid purification apparatus includes the photovoltaic device and the conductive transparent electrodes, thus operating independently, and a PCR buffer can be used as an eluate for the nucleic acid. As a result, the nucleic acid purification apparatus can be integrated, and by using the apparatus, a fast and simplified purification can be obtained. The microfludic apparatus including the nucleic acid purification apparatus can operate independently, and perform the purification of the nucleic acid and a PCR at the same time. As a result, the microfluidic apparatus can be miniaturized and automated.

    摘要翻译： 提供了一种核酸纯化装置，其包括包括入口和出口的室，限定室并形成电场的至少两个电极，向电极施加偏压的光伏器件，包括该电极的微流体装置， 以及使用核酸纯化装置纯化核酸的方法。 核酸纯化装置包括光电器件和导电透明电极，因此独立地操作，并且可以使用PCR缓冲液作为核酸的洗脱液。 结果，可以将核酸纯化装置整合，并且通过使用该装置，可以获得快速和简化的纯化。 包含核酸纯化装置的微循环装置可以独立工作，同时进行核酸的纯化和PCR。 结果，微流控装置可以小型化和自动化。

    Method of identifying target cell using target cell-specific virus
    7.
    发明申请
    Method of identifying target cell using target cell-specific virus 有权
    使用靶细胞特异性病毒鉴定靶细胞的方法

    公开(公告)号：US20060252147A1

    公开(公告)日：2006-11-09

    申请号：US11187409

    申请日：2005-07-22

    申请人： Sang-hyun Peak

    发明人： Sang-hyun Peak

    IPC分类号： C12Q1/68 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12Q1/04 ,  C12Q1/10

    摘要： Provided is a method of identifying a target cell using a virus. The method includes infecting the target cell with the virus by contacting the virus to the target cell and culturing the target cell to propagate the virus; adding a chromogenic substrate to the resultant cell culture to induce enzyme reaction converting the chromogenic substrate to a chromogenic product; and measuring an optical signal emitted from the chromogenic product, wherein the virus contains in its genome a gene encoding an enzyme capable of converting the chromogenic substrate to the chromogenic product and a gene encoding a ligand allowing the virus to specifically bind with a receptor of the target cell to infect the target cell with the virus.

    摘要翻译： 提供了使用病毒识别目标细胞的方法。 该方法包括通过将病毒与靶细胞接触并培养靶细胞以传播病毒，用病毒感染靶细胞; 向所得细胞培养物中加入显色底物以诱导酶反应，将显色底物转化为显色产物; 并测量从显色产物发射的光信号，其中所述病毒在其基因组中含有编码能够将显色底物转化为显色产物的酶的基因和编码配体的基因，所述基因允许病毒与 靶细胞用病毒感染靶细胞。

    Method of identifying target cell using target cell-specific virus
    8.
    发明授权
    Method of identifying target cell using target cell-specific virus 有权
    使用靶细胞特异性病毒鉴定靶细胞的方法

    公开(公告)号：US07364843B2

    公开(公告)日：2008-04-29

    申请号：US11187409

    申请日：2005-07-22

    申请人： Sang-hyun Peak

    发明人： Sang-hyun Peak

    IPC分类号： C12Q1/70 ,  C12Q1/66 ,  C12Q1/42 ,  G01N33/53 ,  G01N33/542

    CPC分类号： C12Q1/04 ,  C12Q1/10

    摘要： Provided is a method of identifying a target cell using a virus. The method includes infecting the target cell with the virus by contacting the virus to the target cell and culturing the target cell to propagate the virus; adding a chromogenic substrate to the resultant cell culture to induce enzyme reaction converting the chromogenic substrate to a chromogenic product; and measuring an optical signal emitted from the chromogenic product, wherein the virus contains in its genome a gene encoding an enzyme capable of converting the chromogenic substrate to the chromogenic product and a gene encoding a ligand allowing the virus to specifically bind with a receptor of the target cell to infect the target cell with the virus.

    摘要翻译： 提供了使用病毒识别目标细胞的方法。 该方法包括通过将病毒与靶细胞接触并培养靶细胞以传播病毒，用病毒感染靶细胞; 向所得细胞培养物中加入显色底物以诱导酶反应，将显色底物转化为显色产物; 以及测量从所述显色产物发射的光信号，其中所述病毒在其基因组中含有编码能够将显色底物转化为显色产物的酶的基因和编码配体的基因，所述基因允许病毒与 靶细胞用病毒感染靶细胞。

    Primer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species
    10.
    发明授权
    Primer set capable of specifically amplifying a target sequence found in 10 bacterial species and probe oligonucleotide specifically hybridizable with each target sequence of the 10 bacterial species 有权
    能够特异性扩增在10种细菌物种中发现的靶序列的引物组和与10种细菌物种的每个靶序列特异性杂交的探针寡核苷酸

    公开(公告)号：US07553626B2

    公开(公告)日：2009-06-30

    申请号：US11317601

    申请日：2005-12-23

    申请人： Ji-young Oh ,  Nam Huh ,  Sang-hyun Peak ,  Sung-young Jeong

    发明人： Ji-young Oh ,  Nam Huh ,  Sang-hyun Peak ,  Sung-young Jeong

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/689

    摘要： Provided are a primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Stapylococcus aureus, and Streptococcus pneumoniae, the primer set including at least one oligonucleotide of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 1 and at least one oligonucleotides of 10 to 100 nucleotides in length, selected from the group consisting of oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in a sequence as set forth in SEQ ID NO: 2; and probes specific to a specific species of the 10 bacterial species.

    摘要翻译： 本发明提供一种引物组，其用于扩增百日盛博氏杆菌，肺炎衣原体，流感嗜血杆菌，肺炎克雷伯杆菌，嗜肺军团杆菌，卡他莫拉菌，肺炎支原体，铜绿假单胞菌，金黄色葡萄球菌和肺炎链球菌特异性的靶序列，所述引物组包括至少一种 长度为10至100个核苷酸的寡核苷酸，选自寡核苷酸，每个寡核苷酸包含存在于如SEQ ID NO：1所示的序列中的至少10个连续核苷酸的片段和10至100个的至少一个寡核苷酸 长度的核苷酸，选自寡核苷酸，每个寡核苷酸包含存在于如SEQ ID NO：2所示的序列中的至少10个连续核苷酸的片段; 并探测10种细菌物种的特定物种。