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24 条记录 (耗时 0.036 秒)

    Cyclin D binding factor, and uses thereof
    1.
    发明授权
    Cyclin D binding factor, and uses thereof 失效
    细胞周期蛋白D结合因子及其用途

    公开(公告)号：US06673902B2

    公开(公告)日：2004-01-06

    申请号：US09892398

    申请日：2001-06-27

    申请人： Charles J. Sherr ,  Hiroshi Hirai ,  Sara M. Bodner ,  Kazushi Inoue

    发明人： Charles J. Sherr ,  Hiroshi Hirai ,  Sara M. Bodner ,  Kazushi Inoue

    IPC分类号： C07K1600

    CPC分类号： C07K14/4705 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00

    摘要： The invention discloses a direct interaction between D-type cyclins and a novel myb-like transcription factor, DMP1, which specifically interacts with cyclin D2. The present invention also provides evidence that D-type cyclins regulate gene expression in an RB-independent manner. Also included is DMP1, the transcription factor composed of a central DNA-binding domain containing three atypical myb repeats flanked by highly acidic segments located at its amino- and carboxyterminal ends. The invention includes amino acid sequences coding for DMP1, and DNA and RNA nucleotide sequences that encode the amino acid sequences. A use of DMP1 as a transcription factor is disclosed due to its specificity in binding to oligonucleotides containing the nonamer consensus sequence CCCG(G/T)ATGT. In this aspect of the invention, DMP1 when transfected into mammalian cells, activates the transcription of a reporter gene driven by a minimal promoter containing concatamerized DMP1 binding sites.

    摘要翻译： 本发明公开了D型细胞周期蛋白与与细胞周期蛋白D2特异性相互作用的新型myb样转录因子DMP1之间的直接相互作用。 本发明还提供了D型细胞周期蛋白以不依赖RB的方式调节基因表达的证据。 还包括DMP1，转录因子由包含三个非典型myb重复的中心DNA结合结构域组成，其侧翼为位于其氨基末端和羧基末端的高度酸性的片段。 本发明包括编码DMP1的氨基酸序列，以及编码氨基酸序列的DNA和RNA核苷酸序列。 公开了DMP1作为转录因子的用途，因为它在与含有非标记共有序列CCCG（G / T）ATGT的寡核苷酸的结合中具有特异性。 在本发明的这个方面，当转染到哺乳动物细胞中时，DMP1激活由含有连接的DMP1结合位点的最小启动子驱动的报道基因的转录。

    Cyclin D binding factor, and uses thereof
    2.
    发明授权
    Cyclin D binding factor, and uses thereof 失效
    细胞周期蛋白D结合因子及其用途

    公开(公告)号：US06180763B2

    公开(公告)日：2001-01-30

    申请号：US08928941

    申请日：1997-09-12

    申请人： Charles J. Sherr ,  Hiroshi Hirai ,  Kazushi Inoue ,  Sara M. Bodner

    发明人： Charles J. Sherr ,  Hiroshi Hirai ,  Kazushi Inoue ,  Sara M. Bodner

    IPC分类号： C07K122

    CPC分类号： C07K14/4705 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00

    摘要： The invention discloses a direct interaction between D-type cyclins and a novel myb-like transcription factor, DMP1, which specifically interacts with cyclin D2. The present invention also provides evidence that D-type cyclins regulate gene expression in an RB-independent manner. Also included is DMP1, the transcription factor composed of a central DNA-binding domain containing three atypical myb repeats flanked by highly acidic segments located at its amino- and carboxyterminal ends. The invention includes amino acid sequences coding for DMP1, and DNA and RNA nucleotide sequences that encode the amino acid sequences. A use of DMP1 as a transcription factor is disclosed due to its specificity in binding to oligonucleotides containing the nonamer consensus sequence CCCG(G/T)ATGT. In this aspect of the invention, DMP1 when transfected into mammalian cells, activates the transcription of a reporter gene driven by a minimal promoter containing concatamerized DMP1 binding sites.

    摘要翻译： 本发明公开了D型细胞周期蛋白与与细胞周期蛋白D2特异性相互作用的新型myb样转录因子DMP1之间的直接相互作用。 本发明还提供了D型细胞周期蛋白以不依赖RB的方式调节基因表达的证据。 还包括DMP1，转录因子由包含三个非典型myb重复的中心DNA结合结构域组成，其侧翼为位于其氨基末端和羧基末端的高度酸性的片段。 本发明包括编码DMP1的氨基酸序列，以及编码氨基酸序列的DNA和RNA核苷酸序列。 公开了DMP1作为转录因子的用途，因为它在与含有非标记共有序列CCCG（G / T）ATGT的寡核苷酸的结合中具有特异性。 在本发明的这个方面，当转染到哺乳动物细胞中时，DMP1激活由含有连接的DMP1结合位点的最小启动子驱动的报道基因的转录。

    Cyclin D binding factor, and uses thereof
    3.
    发明授权
    Cyclin D binding factor, and uses thereof 有权

    公开(公告)号：US06303772B1

    公开(公告)日：2001-10-16

    申请号：US09280590

    申请日：1999-03-29

    申请人： Charles J. Sherr ,  Hiroshi Hirai ,  Sara M. Bodner ,  Kazushi Inoue

    发明人： Charles J. Sherr ,  Hiroshi Hirai ,  Sara M. Bodner ,  Kazushi Inoue

    IPC分类号： C07K122

    CPC分类号： C07K14/4705 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00

    摘要： The invention discloses a direct interaction between D-type cyclins and a novel myb-like transcription factor, DMP1, which specifically interacts with cyclin D2. The present invention also provides evidence that D-type cyclins regulate gene expression in an RB-independent manner. Also included is DMP1, the transcription factor composed of a central DNA-binding domain containing three atypical myb repeats flanked by highly acidic segments located at its amino- and carboxyterminal ends. The invention includes amino acid sequences coding for DMP1, and DNA and RNA nucleotide sequences that encode the amino acid sequences. A use of DMP1 as a transcription factor is disclosed due to its specificity in binding to oligonucleotides containing the nonamer consensus sequence CCCG(G/T)ATGT. In this aspect of the invention, DMP1 when transfected into mammalian cells, activates the transcription of a reporter gene driven by a minimal promoter containing concatamerized DMP1 binding sites.

    InK4c-p18 and InK4d-p19, inhibitors of cyclin-dependent kinases CDK4 and CDK6, and uses thereof
    4.
    发明授权
    InK4c-p18 and InK4d-p19, inhibitors of cyclin-dependent kinases CDK4 and CDK6, and uses thereof 失效
    InK4c-p18和InK4d-p19，细胞周期蛋白依赖性激酶CDK4和CDK6的抑制剂及其用途

    公开(公告)号：US6033847A

    公开(公告)日：2000-03-07

    申请号：US384106

    申请日：1995-02-06

    申请人： Charles J. Sherr ,  James Downing ,  Hiroshi Hirai ,  Tsukasa Okuda

    发明人： Charles J. Sherr ,  James Downing ,  Hiroshi Hirai ,  Tsukasa Okuda

    IPC分类号： G01N33/53 ,  A61K31/70 ,  A61K48/00 ,  A61P35/00 ,  C07K14/47 ,  C07K14/52 ,  C07K16/24 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/574 ,  C07H21/00 ,  C07K14/435 ,  C12N15/85

    CPC分类号： C07K14/47 ,  A01K2217/05 ,  C07K2319/00

    摘要： Members of the InK4 (Inhibitors of CDK4) family inhibit the activities of specific cyclin D-dependent kinases (CDK4 and/or CDK6), thereby arresting cell cycle progression in G1 phase and preventing chromosomal DNA replication. Disclosed herein are novel mammalian InK4 family members, having apparent molecular masses of 18,000 and 19,000 daltons, designated "InK4c-p18" and "InK4d-p19," respectively, or simply "p18" and "p19." In particular, the invention provides p19 genes and proteins isolated from murine or human cells and p18 genes and proteins from murine cells. When constitutively expressed in cells, p19 inhibits cyclin D-dependent kinase activity in vivo and induces G1 phase arrest. Materials and methods disclosed herein include (1) nucleic acids that encode p18 or p19; (2) methods for detecting nucleic acids encoding p18 or p19 proteins; (3) methods for producing p18 or p19 proteins using nucleic acids that encode p18 or p19, respectively; (4) purified pl8 or p19 proteins and peptide fragments, oligopeptides, or fusion proteins derived therefrom; (5) methods of inhibiting cells from replicating their chromosomal DNA using purified p18 or p19 proteins or derivatives thereof; (6) antibodies that specifically bind p18 or p19; (7) methods for detecting p18 and p19 proteins; (8) methods of stimulating cell growth by blocking p18 or p19 expression via antisense oligonucleotides; (9) methods of gene therapy using nucleic acids that encode p18 or p19; and (10) methods of making transgenic non-human animals that have alterations in the gene encoding p18 or p19, or in both genes.

    摘要翻译： InK4（CDK4抑制剂）家族的成员抑制特异性细胞周期蛋白D依赖性激酶（CDK4和/或CDK6）的活性，从而阻止G1期的细胞周期进程并防止染色体DNA复制。 本文公开了新颖的哺乳动物InK4家族成员，其分子量分别为18,000和19,000道尔顿，分别称为“InK4c-p18”和“InK4d-p19”，或简称为“p18”和“p19”。 特别地，本发明提供从鼠或人细胞分离的p19基因和蛋白质，以及来自鼠细胞的p18基因和蛋白质。 当细胞中组成型表达时，p19在体内抑制细胞周期蛋白D依赖性激酶活性，并诱导G1期停滞。 本文公开的材料和方法包括（1）编码p18或p19的核酸; （2）检测编码p18或p19蛋白质的核酸的方法; （3）分别使用编码p18或p19的核酸产生p18或p19蛋白的方法; （4）纯化的p18或p19蛋白质和肽片段，寡肽或从其衍生的融合蛋白; （5）使用纯化的p18或p19蛋白或其衍生物抑制细胞复制染色体DNA的方法; （6）特异性结合p18或p19的抗体; （7）检测p18和p19蛋白的方法; （8）通过反义寡核苷酸阻断p18或p19表达刺激细胞生长的方法; （9）使用编码p18或p19的核酸进行基因治疗的方法; 和（10）制备在编码p18或p19的基因或两种基因中具有改变的转基因非人动物的方法。

    Millimeter wave and far-infrared detector
    6.
    发明授权
    Millimeter wave and far-infrared detector 有权
    毫米波和远红外探测器

    公开(公告)号：US06627914B1

    公开(公告)日：2003-09-30

    申请号：US09763603

    申请日：2001-02-26

    申请人： Susumu Komiyama ,  Astafiev Oleg ,  Antonov Vladimir ,  Hiroshi Hirai ,  Takeshi Kutsuwa

    发明人： Susumu Komiyama ,  Astafiev Oleg ,  Antonov Vladimir ,  Hiroshi Hirai ,  Takeshi Kutsuwa

    IPC分类号： H01L2906

    CPC分类号： H01Q9/28 ,  B82Y10/00 ,  H01L31/02325 ,  H01L31/03046 ,  H01L31/0352 ,  H01L31/101

    摘要： An MR/FIR light detector is disclosed herein that has extraordinarily high degree of sensitivity and a high speed of response. The detector includes an MR/FIR light introducing section (1) for guiding an incident MR/FIR light (2), a semiconductor substrate (14) formed with a single-electron transistor (14) for controlling electric current passing through a semiconductor quantum dot (12) formed therein, and a BOTAI antenna (6, 6a, 6b, 6c) for concentrating the MW/FIR light (2) into a small special zone of sub-micron size occupied by the semiconductor quantum dot (12) in the single-electron transistor (14). The quantum dot (12) forming a two-dimensional electron system absorbs the electromagnetic wave concentrated efficiently, and retains an excitation state created therein for 10 nanoseconds or more, thus permitting electrons of as many as one millions in number or more to be transferred with respect to a single photon absorbed.

    摘要翻译： 本文公开了具有非常高的灵敏度和高响应速度的MR / FIR光检测器。 检测器包括用于引导入射MR / FIR光（2）的MR / FIR光导入部（1），形成有用于控制通过半导体量子的电流的单电子晶体管（14）的半导体衬底（14） 形成在其中的点（12）和用于将MW / FIR光（2）聚集成由半导体量子点（12）占据的亚微米尺寸的小特殊区域的BOTAI天线（6,6a，6b，6c） 单电子晶体管（14）。 形成二维电子系统的量子点（12）吸收有效集中的电磁波，并且将其中产生的激发态保持10纳秒以上，从而允许多达一百万数以上的电子与 尊重单个光子吸收。