公开(公告)号：US20050009165A1

公开(公告)日：2005-01-13

申请号：US10921994

申请日：2004-08-20

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/29 ,  C12N15/82 ,  C07H21/04 ,  C08B37/00 ,  C12N9/24 ,  C12P19/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.

摘要翻译： 从黄瓜纯化的棉子糖合成酶可以作用于蔗糖和肌醇半乳糖苷。 因此，有效地生产棉子糖。 通过用包含棉子糖合成酶基因和在植物中可表达的调节区的嵌合基因转化植物来调节内源棉子糖合成酶的功能。 因此，产生棉子糖家族寡糖减少的植物。

公开(公告)号：US20050066391A1

公开(公告)日：2005-03-24

申请号：US10948251

申请日：2004-09-24

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/24

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US07455996B2

公开(公告)日：2008-11-25

申请号：US11034848

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12P19/18 ,  C12N1/20 ,  C12N9/10 ,  C07H21/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US20050160497A1

公开(公告)日：2005-07-21

申请号：US11034678

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US07250277B2

公开(公告)日：2007-07-31

申请号：US11034678

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12P19/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US20050118300A1

公开(公告)日：2005-06-02

申请号：US11034848

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A23L1/20

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

公开(公告)号：US06891084B1

公开(公告)日：2005-05-10

申请号：US09425055

申请日：1999-10-22

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H5/00 ,  C12N1/21 ,  C12N15/52 ,  C12P21/02

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： The present invention provides a DNA encoding a raffinose synthase from soybean and vectors containing the same. Also provided are plants and host cells transformed with the DNA sequence and methods of altering the levels of raffinose family oligosaccharides in plants.

摘要翻译： 本发明提供编码大豆棉子糖合成酶的DNA和含有它们的载体。 还提供了用DNA序列转化的植物和宿主细胞以及改变植物中棉子糖家族寡糖水平的方法。

公开(公告)号：US6166292A

公开(公告)日：2000-12-26

申请号：US846234

申请日：1997-04-28

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.

摘要翻译： 从黄瓜纯化的棉子糖合成酶可以作用于蔗糖和肌醇半乳糖苷。 因此，有效地生产棉子糖。 通过用包含棉子糖合成酶基因和在植物中可表达的调节区的嵌合基因转化植物来调节内源棉子糖合成酶的功能。 因此，产生棉子糖家族寡糖减少的植物。

公开(公告)号：US06727411B2

公开(公告)日：2004-04-27

申请号：US09727758

申请日：2000-12-04

申请人： Hiroaki Kisaka ,  Takao Kida

发明人： Hiroaki Kisaka ,  Takao Kida

IPC分类号： C12N1529

CPC分类号： C12N9/88 ,  C12N15/8251 ,  C12N15/8253

摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate decarboxylase(GAD) gene is introduced into a plant in anti-sense direction together with an appropriate regulatory sequence, and the expression of GAD gene is supressed.

摘要翻译： 提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法 在该方法中，将谷氨酸脱羧酶（GAD）基因连同适当的调节序列一起引入反义方向的植物中，并且抑制GAD基因的表达。

公开(公告)号：US06969782B2

公开(公告)日：2005-11-29

申请号：US09729821

申请日：2000-12-06

申请人： Hiroaki Kisaka ,  Takao Kida

发明人： Hiroaki Kisaka ,  Takao Kida

IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N15/53 ,  C12N15/82 ,  C12N15/83 ,  C12N15/90 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N9/0004 ,  C12N9/0016 ,  C12N9/93 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12Y104/01002 ,  C12Y104/01004 ,  Y02A40/146

摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

摘要翻译： 提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法 。 在该方法中，将谷氨酸脱氢酶（GDH）基因与适于过度表达植物细胞中编码GDH基因的序列的调节序列一起引入植物。