公开(公告)号：US07455996B2

公开(公告)日：2008-11-25

申请号：US11034848

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12P19/18 ,  C12N1/20 ,  C12N9/10 ,  C07H21/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US20050160497A1

公开(公告)日：2005-07-21

申请号：US11034678

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US20050009165A1

公开(公告)日：2005-01-13

申请号：US10921994

申请日：2004-08-20

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/29 ,  C12N15/82 ,  C07H21/04 ,  C08B37/00 ,  C12N9/24 ,  C12P19/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.

摘要翻译： 从黄瓜纯化的棉子糖合成酶可以作用于蔗糖和肌醇半乳糖苷。 因此，有效地生产棉子糖。 通过用包含棉子糖合成酶基因和在植物中可表达的调节区的嵌合基因转化植物来调节内源棉子糖合成酶的功能。 因此，产生棉子糖家族寡糖减少的植物。

公开(公告)号：US07250277B2

公开(公告)日：2007-07-31

申请号：US11034678

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12P19/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

公开(公告)号：US20050118300A1

公开(公告)日：2005-06-02

申请号：US11034848

申请日：2005-01-14

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A23L1/20

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

公开(公告)号：US06891084B1

公开(公告)日：2005-05-10

申请号：US09425055

申请日：1999-10-22

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H5/00 ,  C12N1/21 ,  C12N15/52 ,  C12P21/02

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： The present invention provides a DNA encoding a raffinose synthase from soybean and vectors containing the same. Also provided are plants and host cells transformed with the DNA sequence and methods of altering the levels of raffinose family oligosaccharides in plants.

摘要翻译： 本发明提供编码大豆棉子糖合成酶的DNA和含有它们的载体。 还提供了用DNA序列转化的植物和宿主细胞以及改变植物中棉子糖家族寡糖水平的方法。

公开(公告)号：US6166292A

公开(公告)日：2000-12-26

申请号：US846234

申请日：1997-04-28

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose synthase purified from cucumber is allowed to act on sucrose and galactinol. Thus raffinose is efficiently produced. The function of endogenous raffinose synthase is regulated by transforming a plant with a chimeric gene comprising a raffinose synthase gene and a regulatory region expressible in the plant. Thus a plant, in which raffinose family oligosaccharides are decreased, is created.

摘要翻译： 从黄瓜纯化的棉子糖合成酶可以作用于蔗糖和肌醇半乳糖苷。 因此，有效地生产棉子糖。 通过用包含棉子糖合成酶基因和在植物中可表达的调节区的嵌合基因转化植物来调节内源棉子糖合成酶的功能。 因此，产生棉子糖家族寡糖减少的植物。

公开(公告)号：US4980285A

公开(公告)日：1990-12-25

申请号：US412562

申请日：1989-09-25

申请人： Konosuke Sano ,  Chieko Osumi ,  Kazuhiko Matsui ,  Kiyoshi Miwa

发明人： Konosuke Sano ,  Chieko Osumi ,  Kazuhiko Matsui ,  Kiyoshi Miwa

IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N15/00 ,  C12N15/52 ,  C12N15/77 ,  C12P13/04 ,  C12P13/08 ,  C12P13/22 ,  C12R1/13 ,  C12R1/15

CPC分类号： C12N15/52 ,  C12N15/77 ,  C12P13/08 ,  C12P13/22 ,  Y10S435/84

摘要： A method for producing an L-amino acid, which comprises inserting a gene which codes for an enzyme which is utilized on the route of biosynthesis of an L-amino acid product into one of at least two plasmid vectors which have compatible replicating origins different from each other, inserting a second gene which codes for an enzyme different from the first enzyme on the route of biosynthesis of the L-amino acid into a second of the plasmid vectors; introducing the thus obtained recombinant plasmids into a strain of Coryneform bacteria; and culturing the thus transformed strain which is capable of producing the L-amino acid, said two enzymes being highly rate determining enzymes for the biosynthesis of L-amino acid.

摘要翻译： 一种L-氨基酸的制造方法，其特征在于，将在L-氨基酸产物的生物合成途径中使用的酶编码的基因插入至少两个具有不同于其的复制起点的质粒载体之一 将在L-氨基酸生物合成途径上编码不同于第一酶的酶的第二基因插入第二质粒载体; 将如此获得的重组质粒导入棒状细菌菌株; 并培养能够产生L-氨基酸的如此转化的菌株，所述两种酶是用于生物合成L-氨基酸的高速率决定酶。

公开(公告)号：US20050066391A1

公开(公告)日：2005-03-24

申请号：US10948251

申请日：2004-09-24

申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/24

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。