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公开(公告)号：US20110281324A1
公开(公告)日：2011-11-17
申请号：US13132252
申请日：2009-11-30
申请人： Christian D. Adams , Kai Bao , Katherine D. Collier , Edwin Lee , Andrei Miasnikov , Zhen Qian , Brian F. Schmidt , Danfeng Song
发明人： Christian D. Adams , Kai Bao , Katherine D. Collier , Edwin Lee , Andrei Miasnikov , Zhen Qian , Brian F. Schmidt , Danfeng Song
CPC分类号： C11D3/38627
摘要： Described are detergent compositions comprising at least one lipase enzyme selected from SriII, ScoIIA, ScoIIB, CefII, and variants, thereof. The compositions are useful for removing oily stains from fabric.
摘要翻译： 描述了包含至少一种选自SriII，ScoIIA，ScoIIB，CefII及其变体的脂肪酶的洗涤剂组合物。 该组合物可用于从织物中除去油性污渍。
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公开(公告)号：US20060154843A1
公开(公告)日：2006-07-13
申请号：US11305650
申请日：2005-12-16
申请人： Huaming Wang , Kai Bao
发明人： Huaming Wang , Kai Bao
CPC分类号： C12N9/2437 , A23K10/14 , A23K20/189 , C11D3/38645 , D06M16/003 , D06M2101/06 , D06M2200/50 , D06P1/38 , D21C5/005
摘要： The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.
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3.发明申请SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL 审中-公开用于改善宿主细胞中蛋白质生产的信号序列和共同表达的CHAPERON公开(公告)号：US20090221030A1
公开(公告)日：2009-09-03
申请号：US12261306
申请日：2008-10-30
申请人： Kai Bao , Huaming Wang
发明人： Kai Bao , Huaming Wang
IPC分类号： C12P21/00
CPC分类号： C12N9/2428 , C07K2319/02 , C12N9/0061 , C12N9/2437 , C12N9/58 , C12N15/62 , C12N15/80 , C12P21/02 , C12Y110/03002 , C12Y302/01091
摘要： The invention provides methods and compositions for improved protein production. The method comprises the steps of: (a) introducing into a host cell a first nucleic acid sequence comprising a signal sequence operably linked to a desired protein sequence; (b) expressing the first nucleic acid sequence; (c) co-expressing a second nucleic acid sequence encoding a chaperone or foldase selected from the group consisting of bip1, ero1, pdi1, tig1, prp1, ppi1, ppi2, prp3, prp4, calnexin, and lhs1; and (d) collecting the desired protein secreted from the host cell. The first nucleic acid sequence optionally comprises an enzyme sequence between the signal sequence and the desired protein sequence.
摘要翻译： 本发明提供了改进蛋白质生产的方法和组合物。 该方法包括以下步骤：（a）向宿主细胞中引入包含可操作地连接到所需蛋白质序列的信号序列的第一核酸序列; （b）表达第一核酸序列; （c）共表达编码选自bip1，ero1，pdi1，tig1，prp1，ppi1，ppi2，prp3，prp4，calnexin和lhs1的分子伴侣或折叠酶的第二核酸序列; 和（d）收集从宿主细胞分泌的所需蛋白。 第一核酸序列任选地包含信号序列和所需蛋白质序列之间的酶序列。
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公开(公告)号：US20090017162A1
公开(公告)日：2009-01-15
申请号：US11784926
申请日：2007-04-09
申请人： Huaming Wang , Kai Bao
发明人： Huaming Wang , Kai Bao
IPC分类号： A23K1/00 , C07H21/04 , C12N15/63 , C12N1/21 , C12N1/15 , C12P21/06 , D21C3/00 , D06M16/00 , C11D3/386
CPC分类号： C12N9/2437 , A23K10/14 , A23K20/189 , C11D3/38645 , D06M16/003 , D06M2101/06 , D06M2200/50 , D06P1/38 , D21C5/005
摘要： The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.
摘要翻译： 本发明涉及在宿主细胞中克隆和高水平表达新的纤维素酶蛋白或其衍生物。 本发明的其它方面涉及表达新型纤维素酶的转化体，以及包含使用遗传工程技术编码来自放线菌的新型纤维素酶的DNA基因片段或其变体的表达载体。 本发明还涉及新型纤维素酶组合物以及因此在工业过程中使用的方法。 特别地，本发明涉及用衍生自放线菌属的新型纤维素酶处理纺织品。 本发明还涉及来自放线菌属的纤维素酶的用途。 以增强动物饲料，洗涤剂，纸浆和纸张的处理和淀粉生产的消化率及其副产物的处理。
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公开(公告)号：US20120057629A1
公开(公告)日：2012-03-08
申请号：US13225222
申请日：2011-09-02
IPC分类号： H04N7/26
CPC分类号： H04N19/52 , H04N5/145 , H04N19/115 , H04N19/124 , H04N19/164 , H04N19/176 , H04N19/198 , H04N19/51 , H04N19/61
摘要： Video encoders, systems and methods are described that characterize video encoding processes using a ρ-domain deviation metric. The deviation metric represents a weighted difference between actual non-zero coefficients and the expected non-zero coefficients, the actual and expected coefficients corresponding to quantization of a macroblock in a video frame during video encoding of the frame. The deviation metric is used to adjust the video encoding process to obtain an optimized encoding bit rate for a desired video encoding quality by selecting a quantizing parameter based on a normalized value of the deviation metric. The quantizing parameter can be selected from a table indexed using the deviation metric.
摘要翻译： 描述了视频编码器，系统和方法，其使用“域名偏差度量”表征视频编码过程。 偏差度量表示实际的非零系数与期望的非零系数之间的加权差，实际和期望的系数对应于帧的视频编码期间视频帧中的宏块的量化。 偏差度量用于通过基于偏差度量的归一化值选择量化参数来调整视频编码处理以获得所需视频编码质量的优化编码比特率。 可以从使用偏差度量索引的表中选择量化参数。
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公开(公告)号：US08824554B2
公开(公告)日：2014-09-02
申请号：US13225269
申请日：2011-09-02
CPC分类号： H04N19/52 , H04N5/145 , H04N19/115 , H04N19/124 , H04N19/164 , H04N19/176 , H04N19/198 , H04N19/51 , H04N19/61
摘要： Video analytics systems and methods are described that typically comprise a video encoder operable to generate macroblock video analytics metadata (VAMD) from a video frame. Functional modules receive the VAMD and an encoded version of the video frame is configured to generate video analytics information related to the frame using the VAMD and the encoded video frame. The downstream decoder can use the VAMD to obtain a global motion vector related to the frame, detect and track motion of an object within the frame and monitor a line provided or found within the frame. Traversals of the line by a moving object can be detected and counted using information in the VAMD and the line may be part of a polygon that delineates an area to be monitored within the encoded frame. The VAMD can comprise macroblock level and video frame level information.
摘要翻译： 描述了视频分析系统和方法，其通常包括可操作以从视频帧生成宏块视频分析元数据（VAMD）的视频编码器。 功能模块接收VAMD，视频帧的编码版本被配置为使用VAMD和编码视频帧生成与帧相关的视频分析信息。 下游解码器可以使用VAMD来获得与帧相关的全局运动矢量，检测和跟踪帧内的对象的运动并监视在帧内提供或发现的线。 可以使用VAMD中的信息来检测和计数运动对象的行的遍历，并且线可以是描绘编码帧内要监视的区域的多边形的一部分。 VAMD可以包括宏块级和视频帧级信息。
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公开(公告)号：US20120057634A1
公开(公告)日：2012-03-08
申请号：US13225269
申请日：2011-09-02
IPC分类号： H04N7/26
CPC分类号： H04N19/52 , H04N5/145 , H04N19/115 , H04N19/124 , H04N19/164 , H04N19/176 , H04N19/198 , H04N19/51 , H04N19/61
摘要： Video analytics systems and methods are described that typically comprise a video encoder operable to generate macroblock video analytics metadata (VAMD) from a video frame. Functional modules receive the VAMD and an encoded version of the video frame is configured to generate video analytics information related to the frame using the VAMD and the encoded video frame. The downstream decoder can use the VAMD to obtain a global motion vector related to the frame, detect and track motion of an object within the frame and monitor a line provided or found within the frame. Traversals of the line by a moving object can be detected and counted using information in the VAMD and the line may be part of a polygon that delineates an area to be monitored within the encoded frame. The VAMD can comprise macroblock level and video frame level information.
摘要翻译： 描述了视频分析系统和方法，其通常包括可操作以从视频帧生成宏块视频分析元数据（VAMD）的视频编码器。 功能模块接收VAMD，视频帧的编码版本被配置为使用VAMD和编码视频帧生成与帧相关的视频分析信息。 下游解码器可以使用VAMD来获得与帧相关的全局运动矢量，检测和跟踪帧内的对象的运动并监视在帧内提供或发现的线。 可以使用VAMD中的信息来检测和计数运动对象的行的遍历，并且线可以是描绘编码帧内要监视的区域的多边形的一部分。 VAMD可以包括宏块级和视频帧级信息。
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公开(公告)号：US20100285567A1
公开(公告)日：2010-11-11
申请号：US12664357
申请日：2008-06-02
申请人： Kai Bao , Fernando Valle
发明人： Kai Bao , Fernando Valle
CPC分类号： C12P21/02 , C07K2319/02 , C12N9/16 , C12N15/625 , C12N15/76
摘要： This invention provides polynucleotides encoding TAT fusion proteins, and methods for producing proteins of interest in a host cell. In particular, the present invention relates to polynucleotides, vectors, polypeptides and methods for expressing organophosphate-degrading enzymes e.g. organophosphorus hydrolase (OPH) in host cell, such as a Streptomyces species host cell.
摘要翻译： 本发明提供了编码TAT融合蛋白的多核苷酸，以及在宿主细胞中产生感兴趣的蛋白质的方法。 特别地，本发明涉及用于表达有机磷酸酯降解酶的多核苷酸，载体，多肽和方法，例如 有机磷水解酶（OPH）在宿主细胞中，如链霉菌属宿主细胞。
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