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    SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL
    1.
    发明申请
    SIGNAL SEQUENCES AND CO-EXPRESSED CHAPERONES FOR IMPROVING PROTEIN PRODUCTION IN A HOST CELL 审中-公开
    用于改善宿主细胞中蛋白质生产的信号序列和共同表达的CHAPERON

    公开(公告)号：US20090221030A1

    公开(公告)日：2009-09-03

    申请号：US12261306

    申请日：2008-10-30

    申请人： Kai Bao ,  Huaming Wang

    发明人： Kai Bao ,  Huaming Wang

    IPC分类号： C12P21/00

    CPC分类号： C12N9/2428 ,  C07K2319/02 ,  C12N9/0061 ,  C12N9/2437 ,  C12N9/58 ,  C12N15/62 ,  C12N15/80 ,  C12P21/02 ,  C12Y110/03002 ,  C12Y302/01091

    摘要： The invention provides methods and compositions for improved protein production. The method comprises the steps of: (a) introducing into a host cell a first nucleic acid sequence comprising a signal sequence operably linked to a desired protein sequence; (b) expressing the first nucleic acid sequence; (c) co-expressing a second nucleic acid sequence encoding a chaperone or foldase selected from the group consisting of bip1, ero1, pdi1, tig1, prp1, ppi1, ppi2, prp3, prp4, calnexin, and lhs1; and (d) collecting the desired protein secreted from the host cell. The first nucleic acid sequence optionally comprises an enzyme sequence between the signal sequence and the desired protein sequence.

    摘要翻译： 本发明提供了改进蛋白质生产的方法和组合物。 该方法包括以下步骤：（a）向宿主细胞中引入包含可操作地连接到所需蛋白质序列的信号序列的第一核酸序列; （b）表达第一核酸序列; （c）共表达编码选自bip1，ero1，pdi1，tig1，prp1，ppi1，ppi2，prp3，prp4，calnexin和lhs1的分子伴侣或折叠酶的第二核酸序列; 和（d）收集从宿主细胞分泌的所需蛋白。 第一核酸序列任选地包含信号序列和所需蛋白质序列之间的酶序列。

    Neutral cellulase catalytic core and method of producing same
    2.
    发明申请
    Neutral cellulase catalytic core and method of producing same 审中-公开
    中性纤维素酶催化核心及其生产方法

    公开(公告)号：US20090017162A1

    公开(公告)日：2009-01-15

    申请号：US11784926

    申请日：2007-04-09

    申请人： Huaming Wang ,  Kai Bao

    发明人： Huaming Wang ,  Kai Bao

    IPC分类号： A23K1/00 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12P21/06 ,  D21C3/00 ,  D06M16/00 ,  C11D3/386

    CPC分类号： C12N9/2437 ,  A23K10/14 ,  A23K20/189 ,  C11D3/38645 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/50 ,  D06P1/38 ,  D21C5/005

    摘要： The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

    摘要翻译： 本发明涉及在宿主细胞中克隆和高水平表达新的纤维素酶蛋白或其衍生物。 本发明的其它方面涉及表达新型纤维素酶的转化体，以及包含使用遗传工程技术编码来自放线菌的新型纤维素酶的DNA基因片段或其变体的表达载体。 本发明还涉及新型纤维素酶组合物以及因此在工业过程中使用的方法。 特别地，本发明涉及用衍生自放线菌属的新型纤维素酶处理纺织品。 本发明还涉及来自放线菌属的纤维素酶的用途。 以增强动物饲料，洗涤剂，纸浆和纸张的处理和淀粉生产的消化率及其副产物的处理。

    Neutral cellulase catalytic core and method of producing same
    3.
    发明申请
    Neutral cellulase catalytic core and method of producing same 审中-公开

    公开(公告)号：US20060154843A1

    公开(公告)日：2006-07-13

    申请号：US11305650

    申请日：2005-12-16

    申请人： Huaming Wang ,  Kai Bao

    发明人： Huaming Wang ,  Kai Bao

    IPC分类号： C11D3/386 ,  C07H21/04 ,  C12P21/06 ,  C12N9/42 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/2437 ,  A23K10/14 ,  A23K20/189 ,  C11D3/38645 ,  D06M16/003 ,  D06M2101/06 ,  D06M2200/50 ,  D06P1/38 ,  D21C5/005

    摘要： The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

    KEX2 cleavage regions of recombinant fusion proteins
    4.
    发明授权
    KEX2 cleavage regions of recombinant fusion proteins 有权
    重组融合蛋白的KEX2切割区

    公开(公告)号：US08518667B2

    公开(公告)日：2013-08-27

    申请号：US13472296

    申请日：2012-05-15

    申请人： Huaming Wang ,  Michael Ward

    发明人： Huaming Wang ,  Michael Ward

    IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N1/20 ,  C07H21/02

    CPC分类号： C07K16/462 ,  C07K16/32 ,  C07K16/46 ,  C07K2319/00 ,  C12N15/62 ,  C12N15/80

    摘要： The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.

    摘要翻译： 本发明涉及包含KEX2区域的融合DNA构建体，所述KEX2区域包含KEX2位点和紧邻KEX2位点5'的KEX2位点前序列，融合多肽，载体和包含融合DNA构建体的细胞，用于产生所需蛋白质的方法 丝状真菌细胞和用于增强从细胞中分泌和/或切割所需蛋白质的方法。

    KEX2 cleavage regions of recombinant fusion proteins
    6.
    发明授权
    KEX2 cleavage regions of recombinant fusion proteins 有权
    重组融合蛋白的KEX2切割区

    公开(公告)号：US08198046B2

    公开(公告)日：2012-06-12

    申请号：US12373121

    申请日：2007-06-21

    申请人： Huaming Wang ,  Michael Ward

    发明人： Huaming Wang ,  Michael Ward

    IPC分类号： C07H21/02 ,  C12P21/06 ,  C12N15/00 ,  C12N1/20

    CPC分类号： C07K16/32 ,  C07K14/37 ,  C07K16/46 ,  C07K2317/56 ,  C07K2319/00 ,  C07K2319/50 ,  C12N15/62 ,  C12N15/80

    摘要： The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.

    摘要翻译： 本发明涉及包含KEX2区域的融合DNA构建体，所述KEX2区域包含KEX2位点和紧邻KEX2位点5'的KEX2位点前序列，融合多肽，载体和包含融合DNA构建体的细胞，用于产生所需蛋白质的方法 丝状真菌细胞和用于增强从细胞中分泌和/或切割所需蛋白质的方法。

    Production of functional antibodies in filamentous fungi
    8.
    发明授权
    Production of functional antibodies in filamentous fungi 有权
    生产丝状真菌中的功能性抗体

    公开(公告)号：US07977067B2

    公开(公告)日：2011-07-12

    申请号：US11007886

    申请日：2004-12-09

    申请人： Scott D. Power ,  Huaming Wang ,  Michael Ward

    发明人： Scott D. Power ,  Huaming Wang ,  Michael Ward

    IPC分类号： C07K16/46

    CPC分类号： C07K16/2818 ,  C07K16/00 ,  C07K16/32 ,  C07K2317/10 ,  C07K2317/41 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2319/00

    摘要： Described herein are methods for the production of monoclonal antibodies in filamentous fungi host cells. The monoclonal antibodies are expressed as full-length fusion proteins that retain functional antigen binding and antibody-dependent cellular cytotoxicity capabilities. Improvements in the cleavage of the glucoamylase-light chain fusion protein to yield a mature antibody are also provided. The antibodies produced in filamentous fungi show equivalent pharmacokinetic disposition to antibodies produced in mammalian cells.

    摘要翻译： 本文描述了在丝状真菌宿主细胞中生产单克隆抗体的方法。 单克隆抗体表达为全长融合蛋白，其保留功能性抗原结合和抗体依赖性细胞毒性能力。 还提供了葡糖淀粉酶 - 轻链融合蛋白切割产生成熟抗体的改进。 在丝状真菌中产生的抗体对哺乳动物细胞中产生的抗体显示出等效的药代动力学位置。

    FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION
    9.
    发明申请
    FILAMENTOUS FUNGI WITH INACTIVATED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION 审中-公开
    FILAMENTOUS FUNGI WITH INVTIVED PROTEASE GENES FOR ALTERED PROTEIN PRODUCTION

    公开(公告)号：US20090253173A1

    公开(公告)日：2009-10-08

    申请号：US12412297

    申请日：2009-03-26

    申请人： Huaming Wang

    发明人： Huaming Wang

    IPC分类号： C12P21/06 ,  C12N15/74 ,  C12N1/15 ,  C12N15/63 ,  C07H21/04

    CPC分类号： C12P21/00 ,  C07K14/38 ,  C12N9/00 ,  C12N9/62 ,  C12N15/80 ,  C12P21/02

    摘要： The invention relates to a filamentous fungal cell (e.g., Aspergillus sp.) comprising at least one inactivated protease gene chosen from apsB, a homolog of apsB, cpsA, a homolog cpsA, and combinations thereof. Nucleic acids and methods for making the inactivated mutant filamentous fungal cells are provided as well as methods for using the cells for the altered production of endogenous or heterologous proteins of interest.

    摘要翻译： 本发明涉及包含至少一种选自apsB的灭活蛋白酶基因，apsB同源物，cpsA，同系物cpsA及其组合的丝状真菌细胞（例如曲霉属）。 提供用于制备灭活的突变丝状真菌细胞的核酸和方法以及使用细胞改变产生感兴趣的内源或异源蛋白质的方法。