利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

10 条记录 (耗时 0.027 秒)

    Method for the treatment of a sample containing biomolecules
    1.
    发明授权
    Method for the treatment of a sample containing biomolecules 有权
    用于处理含有生物分子的样品的方法

    公开(公告)号：US08785120B2

    公开(公告)日：2014-07-22

    申请号：US12862156

    申请日：2010-08-24

    申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 由以下组成的组：a）多胺，b）氨基酸以及寡聚和多肽，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多糖，d）R1R2NR3类型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）碳酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    Method for the treatment of a sample containing biomolecules
    2.
    发明授权
    Method for the treatment of a sample containing biomolecules 有权
    用于处理含有生物分子的样品的方法

    公开(公告)号：US08076069B2

    公开(公告)日：2011-12-13

    申请号：US12477813

    申请日：2009-06-03

    申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 包括a）多胺，b）氨基酸和寡聚和多肽，c）包含这些含氮化合物的含氮杂环化合物，包括均相杂聚物，d）R1R2NR3型胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺，f ）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    METHOD FOR THE TREATMENT OF A SAMPLE CONTAINING BIOMOLECULES
    3.
    发明申请
    METHOD FOR THE TREATMENT OF A SAMPLE CONTAINING BIOMOLECULES 有权
    用于处理含有生物分子样品的方法

    公开(公告)号：US20100323363A1

    公开(公告)日：2010-12-23

    申请号：US12862156

    申请日：2010-08-24

    申请人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian KORFHAGE ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括均匀的杂多聚物，d）R1R2NR3型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）碳酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    Method for the treatment of a sample containing biomolecules
    4.
    发明授权
    Method for the treatment of a sample containing biomolecules 有权
    用于处理含有生物分子的样品的方法

    公开(公告)号：US07838233B2

    公开(公告)日：2010-11-23

    申请号：US11910247

    申请日：2006-04-01

    申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68 ,  C12P21/06 ,  C07K1/00 ,  C07K14/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides In particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡聚物和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多酸，d）R1R2NR3类型的胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5 - 烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺， f）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供一种用于裂解生物样品的方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    Method For the Treatment of a Sample Containing Biomolecules
    5.
    发明申请
    Method For the Treatment of a Sample Containing Biomolecules 有权
    用于处理含有生物分子的样品的方法

    公开(公告)号：US20080187924A1

    公开(公告)日：2008-08-07

    申请号：US11910247

    申请日：2006-04-01

    申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz

    发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Loffert ,  Ralf Himmelreich ,  Claudia Fritz

    IPC分类号： C12Q1/68

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-older heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides In particular a method for the lysis of a biological sample, and methods for the stabilisation of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 来自由a）多胺，b）氨基酸和寡肽和多肽组成的组，c）包含这些含氮化合物的含氮杂环化合物，包括同质杂多酸，d）R 1型胺， 其中R 1，R 2，R 3，R 3，R 3，R 3， SUP>彼此独立地选自由H，C 1 -C 5 - 烷基和 芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺，f）无机铵盐， g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供一种用于裂解生物样品的方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。

    Method and apparatus for the simultaneous, automated decomposition of a plurality of biological samples
    7.
    发明授权
    Method and apparatus for the simultaneous, automated decomposition of a plurality of biological samples 有权
    用于同时自动分解多个生物样品的方法和装置

    公开(公告)号：US09243984B2

    公开(公告)日：2016-01-26

    申请号：US13063805

    申请日：2009-08-06

    申请人： Gerhard Baer ,  Dennis Mertens ,  Andreas Schaefer ,  Friederike Wilmer

    发明人： Gerhard Baer ,  Dennis Mertens ,  Andreas Schaefer ,  Friederike Wilmer

    IPC分类号： B02C19/00 ,  B02C17/14 ,  G01N1/28 ,  G01N1/42

    CPC分类号： G01N1/286 ,  B02C17/14 ,  B02C19/00 ,  G01N1/42 ,  G01N2001/2866

    摘要： The invention relates to a method for decomposing a biological sample (9), the method having the following steps: the sample (9) is put into a container (6) which is composed of plastic, in particular, the container (6) is inserted into an adapter (2, 2a), the adapter with the closed container therein is connected to an apparatus which moves the adapter back and forth, in particular upwards, in automated fashion. This method makes it possible to decompose biological samples in automated fashion, to be precise both samples at room temperature and frozen samples. The invention also relates to an apparatus for carrying out the method, the apparatus having an adapter (2, 2a) which is predominantly composed of plastic and has sleeves (4) which are composed of metal and are intended to accommodate containers (6) which are composed of plastic, in particular.

    摘要翻译： 本发明涉及一种分解生物样品（9）的方法，该方法具有以下步骤：样品（9）放入由塑料制成的容器（6）中，特别是容器（6）为 插入适配器（2,2a）中，其中具有密封容器的适配器连接到使得适配器以自动方式来回移动，特别是向上移动适配器的装置。 这种方法使得可以以自动化方式分解生物样品，准确地说，室温下的样品和冷冻样品。 本发明还涉及一种用于执行该方法的装置，该装置具有主要由塑料构成的适配器（2,2a），并且具有由金属构成并且旨在容纳容器（6）的套筒（4），所述容器 特别是由塑料制成。

    Method And Apparatus For the Simultaneous, Automated Decomposition Of A Plurality Of Biological Samples
    8.
    发明申请
    Method And Apparatus For the Simultaneous, Automated Decomposition Of A Plurality Of Biological Samples 有权
    用于同时自动分解多种生物样品的方法和装置

    公开(公告)号：US20110281259A1

    公开(公告)日：2011-11-17

    申请号：US13063805

    申请日：2009-08-06

    申请人： Gerhard Baer ,  Dennis Mertens ,  Andreas Schaefer ,  Friederike Wilmer

    发明人： Gerhard Baer ,  Dennis Mertens ,  Andreas Schaefer ,  Friederike Wilmer

    IPC分类号： C12Q1/68 ,  C12M1/00

    CPC分类号： G01N1/286 ,  B02C17/14 ,  B02C19/00 ,  G01N1/42 ,  G01N2001/2866

    摘要： The invention relates to a method for decomposing a biological sample (9), said method having the following steps: the sample (9) is put into a container (6) which is composed of plastic, in particular, the container (6) is inserted into an adapter (2, 2a), the adapter with the closed container therein is connected to an apparatus which moves the adapter back and forth, in particular upwards, in automated fashion. This method makes it possible to decompose biological samples in automated fashion, to be precise both samples at room temperature and frozen samples. The invention also relates to an apparatus for carrying out the method, said apparatus comprising an adapter (2, 2a) which is predominantly composed of plastic and has sleeves (4) which are composed of metal and are intended to accommodate containers (6) which are composed of plastic, in particular.

    摘要翻译： 本发明涉及一种分解生物样品（9）的方法，所述方法具有以下步骤：将样品（9）放入由塑料制成的容器（6）中，特别是容器（6）为 插入适配器（2,2a）中，其中具有密封容器的适配器连接到使得适配器以自动方式来回移动，特别是向上移动适配器的装置。 这种方法使得可以以自动化方式分解生物样品，准确地说，室温下的样品和冷冻样品。 本发明还涉及一种用于执行该方法的装置，所述装置包括主要由塑料构成的适配器（2,2a），并具有由金属构成并且用于容纳容器（6）的套筒（4），所述容器 特别是由塑料制成。