公开(公告)号：US07105318B2

公开(公告)日：2006-09-12

申请号：US10322138

申请日：2002-12-17

申请人： Christoph Kessler ,  Gerd Haberhausen ,  Knut Bartl ,  Henrik Orum

发明人： Christoph Kessler ,  Gerd Haberhausen ,  Knut Bartl ,  Henrik Orum

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6823 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q2561/101

摘要： Method for the detection of a nucleic acid comprising the production of a plurality of amplificates of a section of this nucleic acid with the aid of two primers, one of which can bind to a binding sequence A of the nucleic acid and the other can bind to a binding sequence C′ which is complementary to a sequence C which is located in the 3′ direction from A and does not overlap with A, contacting the amplificates with a probe having a binding sequence D which can bind to a sequence B which is located between the sequences A and C or to the complement thereof, and detecting the formation of a hybrid of the amplificate and probe where the sequence located between the binding sequences A and C contains no nucleotides that do not belong to the binding sequence D of the probe or its complement D′.

公开(公告)号：US5001069A

公开(公告)日：1991-03-19

申请号：US256100

申请日：1988-10-04

申请人： Knut Bartl ,  Andreas Dessauer ,  Helmut Lill

发明人： Knut Bartl ,  Andreas Dessauer ,  Helmut Lill

IPC分类号： G01N33/68 ,  C12Q1/56 ,  G01N21/78 ,  G01N33/86

CPC分类号： G01N33/86 ,  C12Q1/56 ,  G01N2333/4626 ,  G01N2333/745 ,  G01N2333/96461

摘要： The present invention provides a process for the photometric determination of protein C and/or protein S activity, especially in plasma, wherein the sample containing the protein C and/or protein S to be determined is incubated with a protein C activator from snake venom with the formation of activated protein C and/or protein S and the decrease of the formation of thrombin from prothrombin brought about by the coagulation factors and the activators thereof is determined by means of a chromogenic thrombin substrate.

摘要翻译： 本发明提供了一种用于光度测定蛋白C和/或蛋白S活性的方法，特别是在血浆中，其中将待测定的含有蛋白C和/或蛋白S的样品与来自蛇毒的蛋白C激活剂一起温育， 活化蛋白C和/或蛋白S的形成以及由凝血因子及其活化剂引起的凝血酶原形成的凝血酶的形成减少是通过显色凝血酶底物来确定的。

公开(公告)号：US4486531A

公开(公告)日：1984-12-04

申请号：US489139

申请日：1983-05-04

申请人： Joachim Ziegenhorn ,  Knut Bartl ,  Max Brandhuber

发明人： Joachim Ziegenhorn ,  Knut Bartl ,  Max Brandhuber

IPC分类号： G01N33/68 ,  C12Q1/44 ,  G01N33/92 ,  C12Q1/60

CPC分类号： C12Q1/44 ,  G01N33/92 ,  G01N2800/044 ,  Y10S435/81 ,  Y10T436/104165 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/25125 ,  Y10T436/25375

摘要： The present invention provides a process for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids by precipitation with polyanions and divalent cations, wherein the precipitation is carried out in the presence of a complex former at a pH value of from 7 to 9.The present invention also provides a reagent for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids, comprising a polyanion, a salt of a divalent metal, a complex former and a substance buffering at pH 7 to 9.

摘要翻译： 本发明提供一种通过用聚阴离子和二价阳离子沉淀直接比浊测定体液中的β-脂蛋白（LDL）的方法，其中沉淀是在复合物的存在下在pH值为7至 本发明还提供用于直接比浊测定体液中的β-脂蛋白（LDL）的试剂，其包含聚阴离子，二价金属的盐，复合物和在pH7至9下缓冲的物质。

公开(公告)号：US4409334A

公开(公告)日：1983-10-11

申请号：US254364

申请日：1981-04-15

申请人： Helmut Lill ,  Knut Bartl

发明人： Helmut Lill ,  Knut Bartl

IPC分类号： C12N9/74 ,  C12N9/96 ,  C12Q1/37 ,  C12Q1/56 ,  G01N33/48 ,  C09K3/00

CPC分类号： C12N9/6429 ,  C12N9/96 ,  C12Q1/56 ,  C12Y304/21005 ,  G01N2333/8117 ,  G01N2333/974 ,  Y10T436/10 ,  Y10T436/105831

摘要： The present invention provides a stabilized thrombin preparation in solid or dissolved form, especially for analytical purposes, containing, as a stabilizer, serum albumin together with at least one protease inhibitor and at least one chelate former.

摘要翻译： 本发明提供了一种固体或溶解形式的稳定凝血酶制剂，特别是用于分析目的，其含有作为稳定剂的血清白蛋白以及至少一种蛋白酶抑制剂和至少一种螯合物。

公开(公告)号：US4931392A

公开(公告)日：1990-06-05

申请号：US355039

申请日：1989-05-15

申请人： Helmut Rehner ,  Knut Bartl ,  Peter Stegmuller ,  Wilhelm Tischer ,  Sibylle Rollinger

发明人： Helmut Rehner ,  Knut Bartl ,  Peter Stegmuller ,  Wilhelm Tischer ,  Sibylle Rollinger

IPC分类号： C12N9/12 ,  C12N9/96 ,  C12Q1/50

CPC分类号： C12Q1/50 ,  C12N9/96

摘要： The present invention provides a process for stabilizing creatine kinase by disulphide modification, wherein creatine kinase is reacted in any desired sequence(a) with a molar excess of disulphide and/or thiosulphonate and(b) with a molar excess of water-soluble carbohydrate as carrier.

摘要翻译： 本发明提供了通过二硫化物修饰来稳定肌酸激酶的方法，其中肌酸激酶以任何所需的序列（a）与摩尔过量的二硫化物和/或硫代磺酸盐反应，和（b）用摩尔过量的水溶性碳水化合物作为 载体

公开(公告)号：US4861712A

公开(公告)日：1989-08-29

申请号：US046659

申请日：1987-05-04

申请人： Knut Bartl ,  Helmut Lill ,  Hans Wielinger

发明人： Knut Bartl ,  Helmut Lill ,  Hans Wielinger

IPC分类号： C12Q1/56 ,  C12Q1/00 ,  G01N33/52 ,  G01N33/86

CPC分类号： G01N33/525 ,  G01N33/86 ,  C12Q2337/12 ,  G01N2333/745 ,  Y10S435/805 ,  Y10S436/81

摘要： The present invention provides an analysis element for the determination of coagulation parameters with the help of a detectable or a detection reaction-initiating substrate of a protease of the blood coagulation system and at least one factor and/or co-factor of the blood coagulation system and a buffer substance, wherein the factor and/or co-factor, together with a water-soluble, non-ionic polymer which does not falsely influence the course of the coagulation cascade, is impregnated on an open, planar composite structure which consists of a material which does not have a disturbing influence on the course of the coagulation.

摘要翻译： 本发明提供了一种用于通过凝血系统的蛋白酶的可检测或检测反应引发底物和血液凝固系统的至少一个因子和/或辅因子来确定凝血参数的分析元件 和缓冲物质，其中所述因子和/或辅助因子与不会对凝血级联进程没有错误影响的水溶性非离子聚合物一起浸渍在开放的平面复合结构上，所述平面复合结构由 不会对凝固过程产生干扰的材料。

公开(公告)号：US4732860A

公开(公告)日：1988-03-22

申请号：US822364

申请日：1986-01-24

申请人： Knut Bartl ,  Helmut Lill

发明人： Knut Bartl ,  Helmut Lill

IPC分类号： G01N33/86 ,  C12Q1/56 ,  G01N21/78

CPC分类号： C12Q1/56 ,  C12Q2337/12 ,  G01N2333/96455

摘要： The present invention provides a process for the photometric determination of prekallikrein in plasma, wherein plasma is incubated with a surface activator, the extinction obtained is measured, a chromogenic thrombin substrate is then added thereto, the optically determinable group liberated therefrom is measured at short time intervals or continuously and the gradient of the linear part of the curve obtained by plotting the time against the extinction is determined as a measure of the prekallikrein content. The partial thromboplastin time of plasma is simultaneously determined by carrying out the incubation in the presence of calcium ions and a phospholipid and measuring the time it takes the extinction to reach a predetermined level.

摘要翻译： 本发明提供了一种用于光度测定血浆中前激肽释放酶的方法，其中将血浆与表面活化剂一起温育，测量所得的消光，然后向其中加入显色凝血酶底物，在短时间内测定从其释放的光学上可测定的基团 间隔或连续，并且通过绘制针对灭绝的时间获得的曲线的线性部分的梯度被确定为前激肽释放素含量的量度。 通过在钙离子和磷脂的存在下进行孵育并测量灭绝达到预定水平的时间，同时测定血浆的部分凝血活酶时间。

公开(公告)号：US4710459A

公开(公告)日：1987-12-01

申请号：US767865

申请日：1985-08-21

申请人： Knut Bartl ,  Helmut Lill

发明人： Knut Bartl ,  Helmut Lill

IPC分类号： G01N33/86 ,  C12Q1/56 ,  G01N33/50 ,  C12N9/68 ,  C12N9/74

CPC分类号： C12Q1/56 ,  C12Q2337/12 ,  G01N2333/75 ,  G01N2333/9726 ,  Y10S435/81

摘要： The present invention provides a process for the determination of fibrin monomer in plasma, wherein a plasma sample containing fibrin monomer and freed from plasmin inhibitors is incubated in buffered solution with tissue plasminogen activator (EPA), plasminogen and a chromogenic plasmin substrate and the color formed is measured as a measure of the amount of fibrin monomer.The present invention also provides a reagent for the determination of fibrin monomer in plasma, wherein it contains plasminogen, tissue plasminogen activator (EPA), chromogenic plasmin substrate and buffer (pH 7.0 to 9.0).

摘要翻译： 本发明提供了一种测定血浆中纤维蛋白单体的方法，其中将含有纤维蛋白单体的血浆样品与纤维蛋白溶酶抑制剂无关的蛋白质在缓冲液中与组织纤溶酶原激活剂（EPA），纤溶酶原和色素性纤维蛋白溶酶基质孵育，并形成颜色 被测量为纤维蛋白单体量的量度。 本发明还提供了用于测定血浆中纤维蛋白单体的试剂，其中它含有纤溶酶原，组织纤溶酶原激活剂（EPA），显色性纤溶酶底物和缓冲液（pH 7.0-9.0）。

公开(公告)号：US4544630A

公开(公告)日：1985-10-01

申请号：US468792

申请日：1983-02-22

申请人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

发明人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/60

CPC分类号： C12Q1/60

摘要： The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.The present invention also provides a reagent for carrying out this process.

摘要翻译： 本发明提供了一种在胆固醇酯酶的作用下，通过胆固醇释放和胆固醇氧化酶氧化释放的胆固醇，在血清脂蛋白的HDL部分存在下，特异性测定LDL级分的胆固醇的方法 和氧气，形成过氧化氢和胆甾烯酮，动力学测量氧化酶反应的一种反应成分的变化，特别是过氧化氢的形成，其中测量在预定时间内进行，反应溶液 其表面活性剂浓度为0.01〜1.5mmol / l，胆固醇酯酶浓度为0.1〜30U / ml。 pH值为6.5〜8.0。 本发明还提供了一种用于进行该方法的试剂。

公开(公告)号：US4409327A

公开(公告)日：1983-10-11

申请号：US233536

申请日：1981-02-11

申请人： Knut Bartl ,  Helmut Lill ,  Peter Roeschlau ,  Joachim Ziegenhorn

发明人： Knut Bartl ,  Helmut Lill ,  Peter Roeschlau ,  Joachim Ziegenhorn

IPC分类号： G01N33/66 ,  C12Q1/37 ,  C12Q1/56 ,  G01N33/50 ,  G01N33/86 ,  C12Q1/38

CPC分类号： G01N33/86 ,  C12Q2337/12 ,  G01N2333/8117 ,  G01N2333/96444 ,  G01N2333/974 ,  G01N2400/40 ,  Y10S435/81

摘要： Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor X.sub.a as the proteolytic enzyme and a chromogen substrate of the latter and measuring the dye liberated from the chromogen substrate in the absence of exogenous anti-thrombin III, the improvement consisting of adding, additionally, at least one compound of the formula ##STR1## wherein X is oxygen or the group NR.sub.5 ;and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen or alkyl of 1 to 2 carbon atoms.

摘要翻译： 通过添加凝血酶或因子Xa作为蛋白水解酶和后者的色底物，测定血浆中肝素生物活性的方法和试剂，并测定在没有外源性抗凝血酶III条件下从色底物释放的染料， 该改进包括另外加入至少一种式（IMAGE）化合物，其中X是氧或NR5基团; 并且R 1，R 2，R 3，R 4和R 5独立地选自氢或1至2个碳原子的烷基。