公开(公告)号：US4544630A

公开(公告)日：1985-10-01

申请号：US468792

申请日：1983-02-22

申请人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

发明人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/60

CPC分类号： C12Q1/60

摘要： The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.The present invention also provides a reagent for carrying out this process.

摘要翻译： 本发明提供了一种在胆固醇酯酶的作用下，通过胆固醇释放和胆固醇氧化酶氧化释放的胆固醇，在血清脂蛋白的HDL部分存在下，特异性测定LDL级分的胆固醇的方法 和氧气，形成过氧化氢和胆甾烯酮，动力学测量氧化酶反应的一种反应成分的变化，特别是过氧化氢的形成，其中测量在预定时间内进行，反应溶液 其表面活性剂浓度为0.01〜1.5mmol / l，胆固醇酯酶浓度为0.1〜30U / ml。 pH值为6.5〜8.0。 本发明还提供了一种用于进行该方法的试剂。

公开(公告)号：US4409328A

公开(公告)日：1983-10-11

申请号：US302861

申请日：1981-09-16

申请人： Joachim Ziegenhorn ,  Knut Bartl ,  Albert Roder ,  Gunter Lang ,  Hans Mollering ,  Ulrich Nagele

发明人： Joachim Ziegenhorn ,  Knut Bartl ,  Albert Roder ,  Gunter Lang ,  Hans Mollering ,  Ulrich Nagele

IPC分类号： G01N33/50 ,  C12N9/04 ,  C12Q1/26 ,  C12Q1/44 ,  C12Q1/61 ,  C12Q1/28 ,  C12Q1/30 ,  C12Q1/32

CPC分类号： C12Q1/61 ,  C12Q1/26 ,  Y10S435/805 ,  Y10S435/81

摘要： For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.

摘要翻译： 为了测定甘油，将后者与半乳糖氧化酶在氧气存在下在水性介质中温育，并测定氧气消耗量或形成的H 2 O 2或甘油醛的量。 适合于此目的的试剂由半乳糖氧化酶和用于测定H 2 O 2的系统或用于测定甘油醛的系统组成。 它还可以含有酯化甘油皂化剂。

公开(公告)号：US4486531A

公开(公告)日：1984-12-04

申请号：US489139

申请日：1983-05-04

申请人： Joachim Ziegenhorn ,  Knut Bartl ,  Max Brandhuber

发明人： Joachim Ziegenhorn ,  Knut Bartl ,  Max Brandhuber

IPC分类号： G01N33/68 ,  C12Q1/44 ,  G01N33/92 ,  C12Q1/60

CPC分类号： C12Q1/44 ,  G01N33/92 ,  G01N2800/044 ,  Y10S435/81 ,  Y10T436/104165 ,  Y10T436/105831 ,  Y10T436/107497 ,  Y10T436/25125 ,  Y10T436/25375

摘要： The present invention provides a process for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids by precipitation with polyanions and divalent cations, wherein the precipitation is carried out in the presence of a complex former at a pH value of from 7 to 9.The present invention also provides a reagent for the direct turbidimetric determination of .beta.-lipoproteins (LDL) in body fluids, comprising a polyanion, a salt of a divalent metal, a complex former and a substance buffering at pH 7 to 9.

摘要翻译： 本发明提供一种通过用聚阴离子和二价阳离子沉淀直接比浊测定体液中的β-脂蛋白（LDL）的方法，其中沉淀是在复合物的存在下在pH值为7至 本发明还提供用于直接比浊测定体液中的β-脂蛋白（LDL）的试剂，其包含聚阴离子，二价金属的盐，复合物和在pH7至9下缓冲的物质。

公开(公告)号：US4409327A

公开(公告)日：1983-10-11

申请号：US233536

申请日：1981-02-11

申请人： Knut Bartl ,  Helmut Lill ,  Peter Roeschlau ,  Joachim Ziegenhorn

发明人： Knut Bartl ,  Helmut Lill ,  Peter Roeschlau ,  Joachim Ziegenhorn

IPC分类号： G01N33/66 ,  C12Q1/37 ,  C12Q1/56 ,  G01N33/50 ,  G01N33/86 ,  C12Q1/38

CPC分类号： G01N33/86 ,  C12Q2337/12 ,  G01N2333/8117 ,  G01N2333/96444 ,  G01N2333/974 ,  G01N2400/40 ,  Y10S435/81

摘要： Method and reagent for the determination of the biological activity of heparin in plasma by adding thrombin or Factor X.sub.a as the proteolytic enzyme and a chromogen substrate of the latter and measuring the dye liberated from the chromogen substrate in the absence of exogenous anti-thrombin III, the improvement consisting of adding, additionally, at least one compound of the formula ##STR1## wherein X is oxygen or the group NR.sub.5 ;and R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen or alkyl of 1 to 2 carbon atoms.

摘要翻译： 通过添加凝血酶或因子Xa作为蛋白水解酶和后者的色底物，测定血浆中肝素生物活性的方法和试剂，并测定在没有外源性抗凝血酶III条件下从色底物释放的染料， 该改进包括另外加入至少一种式（IMAGE）化合物，其中X是氧或NR5基团; 并且R 1，R 2，R 3，R 4和R 5独立地选自氢或1至2个碳原子的烷基。

公开(公告)号：US4301028A

公开(公告)日：1981-11-17

申请号：US110581

申请日：1980-01-09

申请人： Knut Bartl ,  Joachim Ziegenhorn ,  Peter Wunderwald ,  Klaus Beaucamp ,  Helmut Lill

发明人： Knut Bartl ,  Joachim Ziegenhorn ,  Peter Wunderwald ,  Klaus Beaucamp ,  Helmut Lill

IPC分类号： G01N33/66 ,  G01N33/50 ,  G01N33/86 ,  G01N33/96 ,  G01N33/16 ,  C09K3/00

CPC分类号： G01N33/96 ,  G01N2400/40 ,  G01N33/86 ,  Y10T436/10 ,  Y10T436/106664

摘要： A control reagent for heparin activity determination comprising20 to 1500 USP heparin,2000 to 30000 IU antithrombin III,0.15 to 1.5 millimoles serum albumin,0.005 to 0.05 mole ethylenediaminetetraacetic acid sodium salt,0 to 0.4 mole saccharose,2000 to 20000 U aprotinin, andbuffer substance, pH 6.5 to 7.5,per liter of control reagent solution.

摘要翻译： 用于肝素活性测定的对照试剂，其包含20至1500USB肝素，2000至30000IU抗凝血酶III，0.15至1.5毫摩尔血清白蛋白，0.005至0.05摩尔乙二胺四乙酸钠盐，0至0.4摩尔蔗糖，2000至20000U抑肽酶，和 缓冲物质，pH 6.5〜7.5，每升对照试剂溶液。

公开(公告)号：US4234682A

公开(公告)日：1980-11-18

申请号：US13759

申请日：1979-02-21

申请人： Knut Bartl ,  Helmut Lill ,  Joachim Ziegenhorn

发明人： Knut Bartl ,  Helmut Lill ,  Joachim Ziegenhorn

IPC分类号： G01N33/66 ,  C12Q1/56 ,  G01N33/86 ,  C12Q1/38

CPC分类号： C12Q1/56 ,  C12Q2337/12 ,  G01N2333/8117 ,  G01N2333/96444 ,  G01N2333/974 ,  G01N2400/40 ,  Y10T436/108331 ,  Y10T436/143333

摘要： A method for determining biologically active heparin comprising adding, in the absence of exogenous antithrombin III, a proteolytic enzyme selected from the group consisting of thrombin and factor Xa and a chromogen substrate for the enzyme to a heparin sample, measuring the dye released from the chromogen substrate and thus determining the amount of heparin present.

摘要翻译： 一种用于测定生物活性肝素的方法，包括在不存在外源性抗凝血酶III的情况下，将选自凝血酶和因子Xa的蛋白水解酶和所述酶的色原底物加入到肝素样品中，测量从色原体释放的染料 底物，从而确定存在的肝素量。

公开(公告)号：US5001069A

公开(公告)日：1991-03-19

申请号：US256100

申请日：1988-10-04

申请人： Knut Bartl ,  Andreas Dessauer ,  Helmut Lill

发明人： Knut Bartl ,  Andreas Dessauer ,  Helmut Lill

IPC分类号： G01N33/68 ,  C12Q1/56 ,  G01N21/78 ,  G01N33/86

CPC分类号： G01N33/86 ,  C12Q1/56 ,  G01N2333/4626 ,  G01N2333/745 ,  G01N2333/96461

摘要： The present invention provides a process for the photometric determination of protein C and/or protein S activity, especially in plasma, wherein the sample containing the protein C and/or protein S to be determined is incubated with a protein C activator from snake venom with the formation of activated protein C and/or protein S and the decrease of the formation of thrombin from prothrombin brought about by the coagulation factors and the activators thereof is determined by means of a chromogenic thrombin substrate.

摘要翻译： 本发明提供了一种用于光度测定蛋白C和/或蛋白S活性的方法，特别是在血浆中，其中将待测定的含有蛋白C和/或蛋白S的样品与来自蛇毒的蛋白C激活剂一起温育， 活化蛋白C和/或蛋白S的形成以及由凝血因子及其活化剂引起的凝血酶原形成的凝血酶的形成减少是通过显色凝血酶底物来确定的。

公开(公告)号：US07105318B2

公开(公告)日：2006-09-12

申请号：US10322138

申请日：2002-12-17

申请人： Christoph Kessler ,  Gerd Haberhausen ,  Knut Bartl ,  Henrik Orum

发明人： Christoph Kessler ,  Gerd Haberhausen ,  Knut Bartl ,  Henrik Orum

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6823 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q2561/101

摘要： Method for the detection of a nucleic acid comprising the production of a plurality of amplificates of a section of this nucleic acid with the aid of two primers, one of which can bind to a binding sequence A of the nucleic acid and the other can bind to a binding sequence C′ which is complementary to a sequence C which is located in the 3′ direction from A and does not overlap with A, contacting the amplificates with a probe having a binding sequence D which can bind to a sequence B which is located between the sequences A and C or to the complement thereof, and detecting the formation of a hybrid of the amplificate and probe where the sequence located between the binding sequences A and C contains no nucleotides that do not belong to the binding sequence D of the probe or its complement D′.

公开(公告)号：US4409334A

公开(公告)日：1983-10-11

申请号：US254364

申请日：1981-04-15

申请人： Helmut Lill ,  Knut Bartl

发明人： Helmut Lill ,  Knut Bartl

IPC分类号： C12N9/74 ,  C12N9/96 ,  C12Q1/37 ,  C12Q1/56 ,  G01N33/48 ,  C09K3/00

CPC分类号： C12N9/6429 ,  C12N9/96 ,  C12Q1/56 ,  C12Y304/21005 ,  G01N2333/8117 ,  G01N2333/974 ,  Y10T436/10 ,  Y10T436/105831

摘要： The present invention provides a stabilized thrombin preparation in solid or dissolved form, especially for analytical purposes, containing, as a stabilizer, serum albumin together with at least one protease inhibitor and at least one chelate former.

摘要翻译： 本发明提供了一种固体或溶解形式的稳定凝血酶制剂，特别是用于分析目的，其含有作为稳定剂的血清白蛋白以及至少一种蛋白酶抑制剂和至少一种螯合物。

公开(公告)号：US4931392A

公开(公告)日：1990-06-05

申请号：US355039

申请日：1989-05-15

申请人： Helmut Rehner ,  Knut Bartl ,  Peter Stegmuller ,  Wilhelm Tischer ,  Sibylle Rollinger

发明人： Helmut Rehner ,  Knut Bartl ,  Peter Stegmuller ,  Wilhelm Tischer ,  Sibylle Rollinger

IPC分类号： C12N9/12 ,  C12N9/96 ,  C12Q1/50

CPC分类号： C12Q1/50 ,  C12N9/96

摘要： The present invention provides a process for stabilizing creatine kinase by disulphide modification, wherein creatine kinase is reacted in any desired sequence(a) with a molar excess of disulphide and/or thiosulphonate and(b) with a molar excess of water-soluble carbohydrate as carrier.

摘要翻译： 本发明提供了通过二硫化物修饰来稳定肌酸激酶的方法，其中肌酸激酶以任何所需的序列（a）与摩尔过量的二硫化物和/或硫代磺酸盐反应，和（b）用摩尔过量的水溶性碳水化合物作为 载体