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公开(公告)号：US07157262B2
公开(公告)日：2007-01-02
申请号：US10232544
申请日：2002-08-30
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及具有脂肪分解活性的修饰酶，能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA的宿主细胞 序列或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号：US06495357B1
公开(公告)日：2002-12-17
申请号：US09007288
申请日：1998-01-14
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
IPC分类号： C12N920
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及一种具有脂肪分解活性的修饰酶，能够一周期洗涤大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA序列的宿主细胞 或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号：US07851176B2
公开(公告)日：2010-12-14
申请号：US12245082
申请日：2008-10-03
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US07312062B2
公开(公告)日：2007-12-25
申请号：US10779427
申请日：2004-11-22
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schrøder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schrøder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US08679774B2
公开(公告)日：2014-03-25
申请号：US12849911
申请日：2010-08-04
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US07632669B2
公开(公告)日：2009-12-15
申请号：US11951597
申请日：2007-12-06
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12N9/20
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20090047384A1
公开(公告)日：2009-02-19
申请号：US12245082
申请日：2008-10-03
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： A21D8/04
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20080131951A1
公开(公告)日：2008-06-05
申请号：US11951597
申请日：2007-12-06
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
IPC分类号： C12N9/16
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US20100291262A1
公开(公告)日：2010-11-18
申请号：US12849911
申请日：2010-08-04
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US6143545A
公开(公告)日：2000-11-07
申请号：US387922
申请日：1999-09-01
申请人： Ib Groth Clausen , Shamkant Anant Patkar , Kim Borch , Martin Barfoed , Kim Clausen , Claus Crone Fuglsang , Lone Dybdal , Torben Halkier
发明人： Ib Groth Clausen , Shamkant Anant Patkar , Kim Borch , Martin Barfoed , Kim Clausen , Claus Crone Fuglsang , Lone Dybdal , Torben Halkier
CPC分类号： C11B3/003 , A21D8/042 , A23J7/00 , A23K20/189
摘要： The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorus content, wherein said method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of said enzyme for a number of industrial applications.
摘要翻译： 本发明涉及一种降低食用油中含磷成分含量的方法，该方法包括大量不可水合的磷含量，其中所述方法包括使用磷脂酶。 此外，本发明涉及具有磷脂酶活性的酶，编码具有磷脂酶活性的酶的克隆DNA序列，该酶的制备方法以及所述酶在许多工业应用中的用途。
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