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    C. antarctica lipase variants
    6.
    发明授权
    C. antarctica lipase variants 失效
    C.南极洲脂肪酶变体

    公开(公告)号：US06074863A

    公开(公告)日：2000-06-13

    申请号：US360758

    申请日：1994-12-22

    申请人： Allan Svendsen ,  Shamkant Anant Pathar ,  Michi Egel-Mitani ,  Kim Borch ,  Ib Groth Clausen ,  Mogens Trier Hansen

    发明人： Allan Svendsen ,  Shamkant Anant Pathar ,  Michi Egel-Mitani ,  Kim Borch ,  Ib Groth Clausen ,  Mogens Trier Hansen

    IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N1/21 ,  C12N9/20 ,  C12N15/55 ,  C12P7/64 ,  C12R1/19 ,  C12R1/69 ,  C12S3/08 ,  D21C9/08 ,  D21H21/02 ,  C07H21/04 ,  C12N15/63

    CPC分类号： D21H21/02 ,  C12N9/20 ,  C07K2299/00

    摘要： A lipase variant of a parent lipase comprising a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant said amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase. The parent lipase may be a C. antarctica lipase A essentially free from other substances from C. antarctica, which comprises the amino acid sequence shown in SEQ ID No. 2, or a variant of said lipase which (1) has lipase activity, (2) reacts with an antibody reactive with at least one epitope of C. antarctica lipase A having th eamino acid sequence SEQ ID No. 2, and/or (3) is encoded by a nucleotide sequence which hybridizes with an oligonucleotide probe prepared on the basis of the full or partial nucleotide sequence shown in SEQ ID No. 1 encoding the C. antarctica lipase A.

    摘要翻译： PCT No.PCT / DK93 / 00225 Sec。 371日期1994年12月22日第 102（e）日期1994年12月22日PCT提交1993年7月5日PCT公布。 出版物WO94 / 01541 日本1994年1月20日一种包含胰蛋白酶样催化三联体的母体脂肪酶的脂肪酶变体，其包含位于脂肪酶分子的主要疏水性，细长结合口袋中的活性丝氨酸，并位于脂肪酶分子的脂质接触区的临界位置 脂肪酶结构，不同于芳族氨基酸残基的氨基酸残基，该氨基酸残基在水解过程中或期间与脂质底物相互作用，其中脂肪酶变体所述氨基酸残基被芳族氨基酸残基替代，从而赋予 与变体相比，与母体脂肪酶相比具有增加的比活性。 母体脂肪酶可以是基本上不含来自南极洲的其它物质的C.南极洲脂肪酶A，其包含SEQ ID No.2所示的氨基酸序列，或所述脂肪酶的变体（1）具有脂肪酶活性（ 2）与具有氨基酸序列SEQ ID No.2的C.Antarctica脂肪酶A的至少一个表位反应的抗体反应，和/或（3）由与在其上制备的寡核苷酸探针杂交的核苷酸序列编码 基因编码南极洲洲脂肪酶A的SEQ ID No.1所示的全部或部分核苷酸序列。