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公开(公告)号：US07198938B2
公开(公告)日：2007-04-03
申请号：US10883346
申请日：2004-06-30
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
CPC分类号： C12N9/2437 , C12N9/20 , C12N15/01 , C12N15/102 , C12N15/80 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。
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公开(公告)号：US07196189B2
公开(公告)日：2007-03-27
申请号：US10402056
申请日：2003-03-28
CPC分类号： C07K14/38
摘要： The invention provides variant regulator proteins of secondary metabolite production and nucleic acids encoding said variant regulator proteins. In particular, the invention provides variant regulator molecules of the lovE protein.
摘要翻译： 本发明提供次级代谢产物的变体调节蛋白和编码所述变体调节蛋白的核酸。 特别地，本发明提供了lovE蛋白的变体调节剂分子。
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公开(公告)号：US06544774B1
公开(公告)日：2003-04-08
申请号：US09705390
申请日：2000-11-02
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
IPC分类号： C12N114
CPC分类号： C12N9/2437 , C12N9/20 , C12N15/01 , C12N15/102 , C12N15/80 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。
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4.发明授权Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein 失效无毒，非产生，非致病性镰孢表达系统及其中使用的启动子和终止子
公开(公告)号：US5837847A
公开(公告)日：1998-11-17
申请号：US921426
申请日：1997-08-29
IPC分类号： C12N15/09 , C12N1/15 , C12N9/20 , C12N9/24 , C12N9/58 , C12N9/76 , C12N15/80 , C12P21/00 , C12P21/02 , C12R1/77 , C12N15/11
CPC分类号： C12P21/00 , C12N15/80 , C12N9/20 , C12N9/58 , C12N9/6427 , C12P21/02 , Y10S435/929
摘要： The invention is related to a non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium, e.g., Fusarium graminearum host cell comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter. The invention further relates to a method for the production of recombinant proteins using such Fusarium host cells. The invention also relates to a promoter and terminator sequence which may be used in such cells.
摘要翻译： 本发明涉及无毒，非产生，非致病重组镰刀菌，例如禾谷镰孢宿主细胞，其包含编码与启动子可操作连接的异源蛋白质的核酸序列。 本发明还涉及使用这种镰刀菌宿主细胞生产重组蛋白质的方法。 本发明还涉及可用于这种细胞中的启动子和终止子序列。
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5.发明授权Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 有权在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法公开(公告)号：US07332341B2
公开(公告)日：2008-02-19
申请号：US11095491
申请日：2005-03-31
申请人： John C. Royer , Lynne M. Christanson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christanson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
摘要翻译： 本发明涉及包含基因的无标记物修饰的突变体细胞，以及获得和使用这种突变细胞的方法。
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公开(公告)号：US6066493A
公开(公告)日：2000-05-23
申请号：US816239
申请日：1997-03-13
申请人： Jeffrey R. Shuster , John C. Royer
发明人： Jeffrey R. Shuster , John C. Royer
IPC分类号： C12N15/09 , C12N1/15 , C12N1/38 , C12N9/20 , C12N9/42 , C12N15/01 , C12N15/10 , C12N15/80 , C12P21/00 , C12P21/02 , C12R1/645 , C12R1/69 , C12R1/77 , C12N1/14 , C12N13/00 , C12P21/06
CPC分类号： C12N9/2437 , C12N15/01 , C12N15/102 , C12N15/80 , C12N9/20 , C12R1/69 , C12R1/77
摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.
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公开(公告)号：US07163804B1
公开(公告)日：2007-01-16
申请号：US09461537
申请日：1999-12-15
CPC分类号： C12N15/80 , C12N9/20 , C12N9/2437 , C12N9/58 , C12N9/6427 , C12Y302/01004
摘要： The present invention relates non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium host cells of the section Discolor or a teleomorph or synonym thereof, comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
摘要翻译： 本发明涉及Discolor部分或其同义词的无毒非毒性非致病重组镰孢宿主细胞，其包含编码与启动子有效连接的异源蛋白质的核酸序列。
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公开(公告)号：US07157262B2
公开(公告)日：2007-01-02
申请号：US10232544
申请日：2002-08-30
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及具有脂肪分解活性的修饰酶，能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA的宿主细胞 序列或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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9.发明授权Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells 失效在单端孢霉烯缺乏丝状真菌突变体细胞中产生异源多肽的方法公开(公告)号：US06903193B1
公开(公告)日：2005-06-07
申请号：US09710760
申请日：2000-11-10
申请人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
发明人： John C. Royer , Lynne M. Christianson , Gregory A. Gambetta , Howard Brody , Suzanne M. Otani , Wendy T. Yoder
IPC分类号： C12N9/02 , C12N9/06 , C12N9/24 , C12N9/88 , C12N15/80 , A23J35/78 , C07K1/00 , C12N1/14 , C12N9/00 , C12N15/00
CPC分类号： C12N9/0036 , C12N9/0044 , C12N9/2482 , C12N9/88 , C12N15/80 , C12Y302/01008 , C12Y402/03006
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有利于产生多肽的条件下培养亲本丝状真菌细胞的突变体，其中（i）突变细胞包含编码多肽的第一核酸序列 多肽和第二核酸序列，其包含参与单端孢霉烯生产的至少一个基因的修饰，和（ii）当在相同条件下培养时，所述突变体产生比亲本丝状真菌细胞少的单端孢霉素; 和（b）从培养基中分离多肽。 本发明还涉及丝状真菌细胞的突变体以及获得突变细胞，分离的二恶烯合酶和编码二氢合酶的核酸序列的方法。
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公开(公告)号：US06495357B1
公开(公告)日：2002-12-17
申请号：US09007288
申请日：1998-01-14
申请人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人： Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
IPC分类号： C12N920
CPC分类号： C11D3/38627 , C12N9/20
摘要： The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译： 本发明涉及一种具有脂肪分解活性的修饰酶，能够一周期洗涤大量脂肪物质的脂肪分解酶，编码所述酶的DNA序列，包含所述DNA序列的载体，携带所述DNA序列的宿主细胞 或所述载体，以及用于产生具有脂肪分解活性的所述酶的方法。
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