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公开(公告)号:US07098017B2
公开(公告)日:2006-08-29
申请号:US09948080
申请日:2001-09-06
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.
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公开(公告)号:US06300116B1
公开(公告)日:2001-10-09
申请号:US08963851
申请日:1997-11-04
IPC分类号: C12N950
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.
摘要翻译: 本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比,这些酶表现出改善的自体蛋白水解稳定性。
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公开(公告)号:US5837517A
公开(公告)日:1998-11-17
申请号:US642987
申请日:1996-05-06
申请人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
发明人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译: 本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比,酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。 这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。
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公开(公告)号:US06682924B1
公开(公告)日:2004-01-27
申请号:US09587747
申请日:2000-06-05
申请人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
发明人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
IPC分类号: C12N954
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译: 本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比,酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。 这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。
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公开(公告)号:US06190900B1
公开(公告)日:2001-02-20
申请号:US09120577
申请日:1998-07-22
申请人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
发明人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
IPC分类号: C12N954
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译: 本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比,酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。 这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。
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公开(公告)号:US20120165241A1
公开(公告)日:2012-06-28
申请号:US13413870
申请日:2012-03-07
申请人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
发明人: Laurens Nicolaas Sierkstra , Jan Klugkist , Peter Markvardsen , Claus von der Osten , Peter Bauditz
CPC分类号: C12N9/54 , C11D3/386 , C11D3/38681
摘要: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
摘要翻译: 本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比,酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。 这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。
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公开(公告)号:US20090143270A1
公开(公告)日:2009-06-04
申请号:US12360635
申请日:2009-01-27
CPC分类号: C12N9/2417 , C11D3/386 , C11D3/38618 , C12P7/06 , C12P7/14 , C12P19/14 , C12Y302/01001 , Y02E50/17
摘要: The present invention relates to variants (mutants) of parent Termamyl-tike alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
摘要翻译: 本发明涉及亲本Termamyl-tikeα-淀粉酶的变体(突变体),该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质。
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公开(公告)号:US6004790A
公开(公告)日:1999-12-21
申请号:US947965
申请日:1997-10-09
CPC分类号: C12N9/1074 , C12P19/18 , C12Y204/01019
摘要: The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically the invention relates to a method of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.
摘要翻译: 本发明涉及环糊精葡聚糖转移酶的变体。 更具体地,本发明涉及一种通过取代,插入和/或缺失一个或多个氨基酸残基来修饰前体CGT酶的底物结合和/或产物选择性的方法和衍生自前体CGT酶的CGTase变体(s ),其中氨基酸残基保持靠近底物的位置。 此外,本发明涉及编码CGTase变体,表达载体,宿主细胞的DNA构建体和产生本发明的CGTase变体的方法。
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公开(公告)号:US06461849B1
公开(公告)日:2002-10-08
申请号:US09417359
申请日:1999-10-13
申请人: Arne Agerlin Olsen , Claus von der Osten , Kim Vilbour Andersen , Steffen Ernst , Erwin Ludo Roggen
发明人: Arne Agerlin Olsen , Claus von der Osten , Kim Vilbour Andersen , Steffen Ernst , Erwin Ludo Roggen
IPC分类号: C12N950
CPC分类号: C12N9/242 , A61K47/54 , A61K47/60 , A61K47/646 , C11D3/3719 , C11D3/38 , C12N9/54
摘要: The present invention relates to polypeptides with reduced immune response including reduced allergenicity having one or more amino acid residues being substituted with other amino acid residues and/or having coupled one or more polymeric molecules in the vicinity of the polypeptides metal binding site, a method for preparing modified polypeptides of the invention, the use of the polypeptide for reducing the immunogenicity and allergenicity and compositions comprising the polypeptide.
摘要翻译: 本发明涉及具有降低的免疫应答的多肽,包括具有一个或多个氨基酸残基被其它氨基酸残基取代的降低的变应原性和/或在多肽金属结合位点附近具有偶联的一个或多个聚合物分子的方法, 制备本发明的修饰多肽,多肽用于降低免疫原性和变应原性的用途以及包含该多肽的组合物。
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公开(公告)号:US5914306A
公开(公告)日:1999-06-22
申请号:US140008
申请日:1993-10-22
CPC分类号: C11D3/38645 , C11D3/386 , C11D3/38627 , C11D3/38636 , C11D3/38654 , C12N9/00 , C12N9/20
摘要: This invention relates to novel stabilized enzymes. More specifically the invention relates to novel stabilized enzymes, in which a naturally occurring amino acid residue (other than proline) has been substituted with a proline residue at one or more positions; at which position(s) the dihedral angles .phi. (phi) constitute values within the interval �-90.degree.
摘要翻译: PCT No.PCT / DK92 / 00142 Sec。 371日期:1993年10月22日 102(e)日期1993年10月22日PCT提交1992年5月1日PCT公布。 出版物WO92 / 19726 日期:1992年11月12日本发明涉及新型稳定化酶。 更具体地,本发明涉及新的稳定化酶,其中天然存在的氨基酸残基(除脯氨酸以外)已被一个或多个位置的脯氨酸残基取代; 在哪个位置上二面角phi(phi)构成间隔[-90°
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