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公开(公告)号：US10510436B2
公开(公告)日：2019-12-17
申请号：US15201623
申请日：2016-07-05
发明人： Jr. Winston Wong , Stephen Chang-Chi Kao , Ying-Ta Lai , Yih-Jyh Shann , Ming-Fa Chen , Chih-Rong Chen
摘要： The present invention discloses a method of real-time quantification of a target nucleic acid in a sample by constructing a reference table of copy number vs. designated parameter from reference samples which sharing the same nucleic acid sequences with the target nucleic acid. The method includes (a) constructing a reference table of copy number vs. designated parameter from reference samples; (b) amplifying the target nucleic acid; (c) monitoring and detecting the amplification of the target nucleic acid in real-time; (d) analyzing the detected signals to get the designated parameter of the target nucleic acid; and (e) looking up and interpolating to the reference table to get the copy number of the target nucleic acid.
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公开(公告)号：US10222307B2
公开(公告)日：2019-03-05
申请号：US15586302
申请日：2017-05-04
IPC分类号： B01D63/16 , B01F9/00 , G01N1/00 , G01N1/44 , G01N1/02 , G01N1/38 , B01F15/06 , G01N1/40 , G01N33/558 , B01F7/00 , G01N21/75 , G01N35/00
摘要： A mixing device for operating biological, chemical or biochemical materials used in an assay includes a mixing member formed with a plurality of chambers, each having a sealable port provided along an edge of the mixing member. The mixing device also includes one or more compartments that are movable along the edge of the mixing member between selected ones of the sealed chambers. This compartment is operable to receive materials from and transfer materials between the chambers. Selected ones of the chambers include associated processing elements, for example, including heating and cooling elements, magnetic elements, membranes and lateral flow devices. The mixing device is also pivotable, for example, to facilitate the application of gravity force in the transfer of materials between the chambers and one or more compartments. The mixing device may operate manually by hand-held unit. Also, this mixing device may operate automatically with at least one driving unit.
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公开(公告)号：US09816136B1
公开(公告)日：2017-11-14
申请号：US15614626
申请日：2017-06-06
CPC分类号： C12Q1/686 , B01L3/502 , B01L7/52 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/6851 , C12Q2537/149 , C12Q2547/101 , C12Q2565/625
摘要： The present invention discloses a two-stage reaction and detection tube comprises a first tube, a second tube and a connector. The first tube comprises a detection space for placing a dipstick and a detection space for the test result. The second tube comprises a storing space for the PCR or RT-PCR reagents and the target gene segments. The connector comprises a first portion and a second portion which connect to the first tube and the second tube respectively. The connector further comprises a diversion unit, a liquid collection space, and a dipstick fixing space, where the liquid collection space is connected to the dipstick fixing space. The target gene amplification and detection could be directly processed in the same tube without any liquid transfer.
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公开(公告)号：US20190193080A1
公开(公告)日：2019-06-27
申请号：US16221562
申请日：2018-12-16
发明人： Ying-Ta Lai , Yu-Cheng Ou , Ming-Lung Hung , Cheng-Yueh Chung , Han-Yi Chen
IPC分类号： B01L7/00 , C12Q1/686 , C12Q1/6851
CPC分类号： B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/025 , B01L2300/0663 , B01L2300/1805 , B01L2300/1827 , B01L2300/1844 , C12Q1/6844 , C12Q1/6851 , C12Q1/686 , C12Q2527/101 , C12Q2527/15
摘要： The present invention discloses a convective PCR apparatus by using a transparent conductive thin film to replace the traditional metal heater. The PCR reaction is activated when the container with reagents contacted the heated transparent conductive thin film and the temperature inside the container raised to initiate the convective circulation. Also, the present invention could apply for a quantitative PCR reaction by adding a specific probe, fluorescent dye, light source, or photon receiver.
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公开(公告)号：US20170336304A1
公开(公告)日：2017-11-23
申请号：US15586302
申请日：2017-05-04
IPC分类号： G01N1/38 , B01F9/00 , B01F15/06 , G01N33/558 , G01N1/44 , G01N1/40 , G01N1/02 , B01D63/16 , G01N1/00
CPC分类号： G01N1/38 , B01D63/16 , B01F9/0003 , B01F15/06 , B01F2015/061 , B01F2215/0037 , G01N1/02 , G01N1/405 , G01N1/44 , G01N33/558 , G01N2001/002 , G01N2001/386 , G01N2035/00564
摘要： A mixing device for operating biological, chemical or biochemical materials used in an assay includes a mixing member formed with a plurality of chambers, each having a sealable port provided along an edge of the mixing member. The mixing device also includes one or more compartments that are movable along the edge of the mixing member between selected ones of the sealed chambers. This compartment is operable to receive materials from and transfer materials between the chambers. Selected ones of the chambers include associated processing elements, for example, including heating and cooling elements, magnetic elements, membranes and lateral flow devices. The mixing device is also pivotable, for example, to facilitate the application of gravity force in the transfer of materials between the chambers and one or more compartments. The mixing device may operate manually by hand-held unit. Also, this mixing device may operate automatically with at least one driving unit.
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6.发明授权公开(公告)号：US10233509B2
公开(公告)日：2019-03-19
申请号：US15371223
申请日：2016-12-07
IPC分类号： A61K39/395 , C07H21/04 , C12N15/00 , C07H21/02 , A61K39/00 , C12Q1/70 , C12Q1/6823
摘要： The present invention disclose a method for detection CPV 2a, 2b, and 2c in a sample by detecting VP2 gene. The signals can be detected by both fluorescent detection system and lateral flow immunochromatographic assay. The second object of this present invention is to provide a method to discriminate the wild type from the vaccine type. The first approach to achieve this object is discriminating wild type from vaccine type by SNP 36, which could be used on both fluorescent detection system and lateral flow immunochromatographic assay. The second approach to achieve this object is discriminating wild type from vaccine type by SNP 899 and SNP 963.
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7.发明申请公开(公告)号：US20180155797A1
公开(公告)日：2018-06-07
申请号：US15371223
申请日：2016-12-07
CPC分类号： C12Q1/701 , C12Q1/6823 , C12Q2600/112 , C12Q2600/156 , C12Q2600/158
摘要： The present invention disclose a method for detection CPV 2a, 2b, and 2c in a sample by detecting VP2 gene. The signals can be detected by both fluorescent detection system and lateral flow immunochromatographic assay. The second object of this present invention is to provide a method to discriminate the wild type from the vaccine type. The first approach to achieve this object is discriminating wild type from vaccine type by SNP 36, which could be used on both fluorescent detection system and lateral flow immunochromatographic assay. The second approach to achieve this object is discriminating wild type from vaccine type by SNP 899 and SNP 963.
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公开(公告)号：US09873910B1
公开(公告)日：2018-01-23
申请号：US15730757
申请日：2017-10-12
CPC分类号： C12Q1/686 , B01L3/502 , B01L7/52 , C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q1/6851 , C12Q2537/149 , C12Q2547/101 , C12Q2565/625
摘要： The present invention provides a method of using a two-stage nucleic acid reaction and detection tube. First, a reaction and detection tube is provided. Then a reaction is performed by using a liquid in the storing space of the second tube, following by putting the dipstick into the dipstick fixing space of the connector. The first tube, the second tube and the connector is assembled together, wherein the dipstick is placed both in the dipstick fixing space of the connector and the detection space of the first tube. The reaction and detection tube is rotated from bottom to top, thus making the liquid in the storing space of the second tube flow into the liquid collection space through the diversion unit and then contact the dipstick in the dipstick fixing space. A detection result is then observed from the dipstick.
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公开(公告)号：US20180011967A1
公开(公告)日：2018-01-11
申请号：US15201623
申请日：2016-07-05
发明人： Jr Winston WONG , Stephen, Chang-Chi KAO , Ying-Ta Lai , Yih-Jyh Shann , Ming-Fa Chen , Chih-Rong Chen
CPC分类号： G16B30/00 , C12Q1/6851 , C12Q1/686 , G16B25/00 , G16B25/20 , C12Q2537/16 , C12Q2537/165 , C12Q2561/113
摘要： The present invention discloses a method of real-time quantification of a target nucleic acid in a sample by constructing a reference table of copy number vs. designated parameter from reference samples which sharing the same nucleic acid sequences with the target nucleic acid. After that, obtain the designated parameter of the target sample and get the copy number by looking up and interpolating to the reference table. The object of the present invention is in particular provide methods for the quantification of the target nucleic acid which the target nucleic acid is quantified independently without comparing it to the standard controls by using a calibration curve. This invention will not only provide a new quantifying method, but will also propose a new standard operational method that eliminates the variations accompanying amplification efficiency, polymerase activity, primer concentrations, and instrument variations.
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公开(公告)号：US11565268B2
公开(公告)日：2023-01-31
申请号：US16221562
申请日：2018-12-16
发明人： Ying-Ta Lai , Yu-Cheng Ou , Ming-Lung Hung , Cheng-Yueh Chung , Han-Yi Chen
IPC分类号： B01L7/00 , C12Q1/6851 , C12Q1/686 , C12Q1/6844
摘要： The present invention discloses a convective PCR apparatus by using a transparent conductive thin film to replace the traditional metal heater. The PCR reaction is activated when the container with reagents contacted the heated transparent conductive thin film and the temperature inside the container raised to initiate the convective circulation. Also, the present invention could apply for a quantitative PCR reaction by adding a specific probe, a fluorescent dye, a light source, or a photon receiver.
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