公开(公告)号：US20230227881A1

公开(公告)日：2023-07-20

申请号：US18154437

申请日：2023-01-13

Applicant: CureVac SE

Inventor： Tilmann ROOS ,  Benyamin YAZDAN PANAH ,  Markus CONZELMANN ,  Andreas THESS ,  Dominik BUOB ,  Martin KUNZE ,  Veronika WAGNER

IPC: C12P19/34 ,  C12N9/10 ,  C12N9/16 ,  C12N9/12 ,  C12N11/02 ,  C12N11/087 ,  C12N11/098

CPC classification number: C12P19/34 ,  C12N9/1007 ,  C12Y201/01057 ,  C12Y201/01056 ,  C12Y301/03033 ,  C12N9/16 ,  C12Y207/0705 ,  C12N9/1241 ,  C12N11/02 ,  C12N11/087 ,  C12N11/098

Abstract: The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.

公开(公告)号：US20240100142A1

公开(公告)日：2024-03-28

申请号：US18315781

申请日：2023-05-11

Applicant: CureVac SE

Inventor： Tilmann ROOS ,  Martin KUNZE ,  Benyamin YAZDAN PANAH ,  Salih YILMAZ ,  Markus CONZELMANN

IPC: A61K39/12 ,  A61P21/00 ,  A61P31/00 ,  A61P39/00 ,  C07K14/005 ,  C12N15/10 ,  C12Q1/6844 ,  C40B40/02 ,  C40B40/08

CPC classification number: A61K39/12 ,  A61P21/00 ,  A61P31/00 ,  A61P39/00 ,  C07K14/005 ,  C12N15/1024 ,  C12Q1/6844 ,  C40B40/02 ,  C40B40/08 ,  A61K2039/53

Abstract: The invention relates to a method for producing a ribonucleic acid (RNA) molecule composition comprising n different RNA molecule species, the method comprising a step of RNA in vitro transcription of a mixture of m different deoxyribonucleic acid (DNA) molecule species in a single reaction vessel in parallel, i.e. simultaneously, and a step of obtaining the RNA molecule composition. Also provided is the RNA composition provided by the inventive method and a pharmaceutical composition comprising the same as well as a pharmaceutical container. Moreover, the invention provides the RNA composition and the pharmaceutical composition for use as medicament.