公开(公告)号：US12110378B2

公开(公告)日：2024-10-08

申请号：US16980025

申请日：2019-03-12

申请人： Centre national de la recherche scientifique ,  UNIVERSITE GRENOBLE ALPES ,  Commissariat à l'énergie atomique et aux énergies alternatives

发明人： Alice Nicolas ,  Camille Migdal

IPC分类号： C08J9/36 ,  B81C1/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08J7/04 ,  C12N11/089 ,  C12N11/098

CPC分类号： C08J9/36 ,  B81C1/00206 ,  C08J7/04 ,  C08J9/365 ,  C12N11/089 ,  C12N11/098 ,  B82Y30/00 ,  B82Y40/00 ,  C08J2205/022 ,  C08J2205/042 ,  C08J2205/10 ,  C08J2333/26 ,  C08J2389/00

摘要： The invention relates to a method for depositing nano-objects on the surface of a gel comprising the steps of: a) providing a gel having a polymer matrix and a solvent within the polymer matrix, the polymer matrix forming a three-dimensional network which is capable of swelling in the presence of the solvent, wherein the solubility of the polymer matrix in the solvent at 1 bar and 25° C. is less than 1 g/l, wherein the gel has a rigidity gradient on the micrometer scale of less than 10%, then b) depositing nano-objects on the surface of the gel, the nano-objects having a mean diameter greater than or equal to the mean diameter of the pores of the gel, then c) evaporating the solvent from the gel at least until the content of solvent no longer varies over time, under the proviso that, at the start of evaporation, the content of mineral salts in the solvent is less than 6 g/l, the gel capable of being obtained and the uses thereof.

公开(公告)号：US20230227881A1

公开(公告)日：2023-07-20

申请号：US18154437

申请日：2023-01-13

申请人： CureVac SE

发明人： Tilmann ROOS ,  Benyamin YAZDAN PANAH ,  Markus CONZELMANN ,  Andreas THESS ,  Dominik BUOB ,  Martin KUNZE ,  Veronika WAGNER

IPC分类号： C12P19/34 ,  C12N9/10 ,  C12N9/16 ,  C12N9/12 ,  C12N11/02 ,  C12N11/087 ,  C12N11/098

CPC分类号： C12P19/34 ,  C12N9/1007 ,  C12Y201/01057 ,  C12Y201/01056 ,  C12Y301/03033 ,  C12N9/16 ,  C12Y207/0705 ,  C12N9/1241 ,  C12N11/02 ,  C12N11/087 ,  C12N11/098

摘要： The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.

公开(公告)号：US20240101948A1

公开(公告)日：2024-03-28

申请号：US17955463

申请日：2022-09-28

申请人： United States of America as Represented by The Secretary of the Army

发明人： Seung J. OH ,  Tanner J. Wood

IPC分类号： C12M1/26 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  C12M1/12 ,  C12N11/098

CPC分类号： C12M33/04 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  C12M25/02 ,  C12N11/098 ,  C12N2537/10

摘要： A 3D bioprinter having a visible light source to photocure biomaterial is disclosed. The 3D bioprinter prints visible light-curable biomaterial along with viable cells, and visible light photocures the biomaterial while maintaining cell viability. Visible light 3D bioprinter systems and methods of printing are further disclosed.

公开(公告)号：US12084649B2

公开(公告)日：2024-09-10

申请号：US17587006

申请日：2022-01-28

申请人： CORNELL UNIVERSITY

发明人： Stephane C. Corgie ,  Xiaonan Duan ,  Emmanuel Giannelis ,  Daniel Aneshansley ,  Larry P. Walker

IPC分类号： C12N11/04 ,  A62D3/02 ,  B01J8/00 ,  B01J19/12 ,  B01J31/00 ,  B01J31/02 ,  B01J35/33 ,  B01J35/56 ,  B01J37/02 ,  C02F1/48 ,  C02F3/00 ,  C02F3/34 ,  C07C1/12 ,  C07D301/22 ,  C07G1/00 ,  C08F14/00 ,  C08F18/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N11/08 ,  C12N11/091 ,  C12N11/098 ,  C12N11/14 ,  C12N13/00 ,  C12P7/04 ,  C12P7/22 ,  C12P17/02 ,  B01J35/64 ,  C02F101/32

CPC分类号： C12N11/04 ,  A62D3/02 ,  B01J8/00 ,  B01J19/12 ,  B01J31/003 ,  B01J31/02 ,  B01J35/33 ,  B01J35/56 ,  B01J37/02 ,  C02F1/484 ,  C02F3/00 ,  C02F3/342 ,  C07C1/12 ,  C07D301/22 ,  C07G1/00 ,  C08F14/00 ,  C08F18/00 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0061 ,  C12N9/0065 ,  C12N11/08 ,  C12N11/091 ,  C12N11/098 ,  C12N11/14 ,  C12N13/00 ,  C12P7/04 ,  C12P7/22 ,  C12P17/02 ,  B01J35/647 ,  B01J35/651 ,  B01J2208/00805 ,  B01J2219/0854 ,  B01J2219/0862 ,  C02F2101/327 ,  C02F2209/006 ,  C02F2303/04 ,  C02F2305/08 ,  C12Y101/03004 ,  C12Y111/01 ,  Y02P20/50 ,  Y02W10/40

摘要： A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

公开(公告)号：US20240209404A1

公开(公告)日：2024-06-27

申请号：US18576208

申请日：2021-10-13

申请人： TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES

发明人： Yanhe MA ,  Ting SHI ,  Pingping HAN

IPC分类号： C12P19/02 ,  C12N1/20 ,  C12N11/089 ,  C12N11/098

CPC分类号： C12P19/02 ,  C12N1/20 ,  C12N11/089 ,  C12N11/098

摘要： Provided are a preparation method for an immobilized cell for tagatose production and a method for producing tagatose by using the immobilized cell. The preparation method for the immobilized cell comprises: mixing a fermentation broth of Escherichia coli or Bacillus subtilis that expresses α-glucan phosphorylase, phosphoglucomutase, glucose phosphate isomerase, tagatose 6-phosphate epimerase, and tagatose 6-phosphate phosphatase to obtain a fermentation mixture, adding inorganic soil and then performing uniform stirring, then adding a flocculant to flocculate bacteria, subsequently adding a cross-linking agent to cross-link, performing vacuum filtration to obtain a filter cake, using a rotary granulator to extrude the filter cake to granulate into a long strip, then cutting by means of a spherical shot blasting machine into particles having equal lengths, and performing boiling drying to obtain the immobilized cell for tagatose production. According to the present invention, separation and purification steps of an enzyme required in tagatose production are simplified, the recycling rate of the enzyme is improved, and the recycling of the enzyme is achieved.