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公开(公告)号：US11333597B2
公开(公告)日：2022-05-17
申请号：US15942430
申请日：2018-03-30
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase , Wenbin Jiang
IPC: G01N15/14 , G01J3/02 , G01J3/28 , G01J3/36 , G01N21/64 , G01J3/44 , G01J3/18 , G01J3/14 , G01N21/53 , G01J3/12 , G01N15/00 , G01N15/10
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
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公开(公告)号：US20180024040A1
公开(公告)日：2018-01-25
申请号：US15659610
申请日：2017-07-25
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
CPC classification number: G01N15/1436 , G01J1/08 , G01J3/0208 , G01J3/021 , G01J3/0218 , G01J3/0256 , G01J3/0291 , G01J3/36 , G01J2003/1213 , G01N15/1434 , G01N15/1459 , G01N21/47 , G01N2015/1006 , G01N2015/145
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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3.发明公开公开(公告)号：US20230168178A1
公开(公告)日：2023-06-01
申请号：US17994395
申请日：2022-11-28
Applicant: CYTEK BIOSCIENCES, INC.
IPC: G01N15/14
CPC classification number: G01N15/1434 , G01N15/1404 , G01N2015/1006
Abstract: An optical fiber forward scatter channel in a flow cytometer is disclosed. A detector system in the flow cytometer includes fiber optic cable for receiving scattered light from an incident laser light that is directed at cells/particles passing through the flow cytometer. The fiber optic cable delivers the scattered light to a sensor system, which collects data to perform analyses on the scattered light. Such analyses may include, for example, calculating the size of a cell/particle, counting cells/particles, and so on. The fiber optic cable is an inherently efficient and accurate filter for the acceptance or rejection of the scattered light.
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公开(公告)号：US20220128450A9
公开(公告)日：2022-04-28
申请号：US15942430
申请日：2018-03-30
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
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公开(公告)号：US20210262917A1
公开(公告)日：2021-08-26
申请号：US17302651
申请日：2021-05-10
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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Patent Agency Ranking
公开(公告)号：US11029243B2
公开(公告)日：2021-06-08
申请号：US15659610
申请日：2017-07-25
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC: G01N15/00 , G01N21/00 , G01J3/00 , G01N15/14 , G01J1/08 , G01N21/47 , G01J3/02 , G01J3/36 , G01N15/10 , G01J3/12
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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7.发明公开公开(公告)号：US20230405592A1
公开(公告)日：2023-12-21
申请号：US18297615
申请日：2023-04-08
Applicant: Cytek Biosciences, Inc.
Inventor： Kuncheng Wang , Huimin Gu , Andrew Klyce-Mariscal , Wing Lee , Ming Yan , Glen Krueger , David Vrane
CPC classification number: B01L3/502746 , G01N15/1434 , G01N2015/149 , G01N2015/1006
Abstract: A flow cell subassembly includes a carriage assembly coupled to a flow cell body to selectively engage a nozzle with a base of a cuvette. The carriage assembly includes a linear bearing slidingly engaged with the flow cell body, a tiltable carriage plate coupled to the linear bearing, and a nozzle mount coupled to the tiltable carriage plate. The nozzle mount receives a nozzle assembly with the nozzle. Set screws can adjust pitch angle of the tiltable carriage plate with the linear bearing to adjust engagement between the nozzle and cuvette in a first dimension. To adjust engagement between the nozzle and cuvette in a second dimension, axial play in bolts/screws through the tiltable carriage plate into threaded holes of the linear bearing allow for yaw angle adjustments.
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8.发明公开公开(公告)号：US20230288313A1
公开(公告)日：2023-09-14
申请号：US18319048
申请日：2023-05-17
Applicant: Cytek Biosciences, Inc.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
CPC classification number: G01N15/1436 , G01J1/08 , G01N21/47 , G01N15/1459 , G01N15/1434 , G01J3/0218 , G01J3/0291 , G01J3/0256 , G01J3/36 , G01J3/021 , G01J3/0208 , G01N2015/145 , G01N2015/1006
Abstract: A method includes launching, from an optical fiber, fluorescent light of differing wavelengths generated by different fluorochromes attached to particles in a sample fluid; magnifying an image size from an end of the optical fiber to a first dichroic filter of a row of a plurality of dichroic filters in a de-multiplexing imaging array; alternatively reflecting the fluorescent light between the plurality of dichroic filters and a plurality of micro-mirrors to collimate the fluorescent light on odd numbered dichroic filters and re-image the fluorescent light on even numbered dichroic filters; band passing different wavelength ranges of the fluorescent light at each of the plurality of dichroic filters to de-multiplex the wavelength spectrum of the wavelength range of the fluorescent light; and detecting fluorescent light in each of the different wavelength ranges to count a number of each of the different particles in the sample fluid.
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公开(公告)号：US11513055B2
公开(公告)日：2022-11-29
申请号：US17022621
申请日：2020-09-16
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Eric Chase , Yung-Chieh Hsieh
Abstract: A crystal for flow cytometry with dual laser beams is disclosed. The crystal is a birefringent crystal comprising a material composition including a quartz mineral having a face side including a face angle of ninety degrees plus or minus one tenth of a degree; a wedge side that is substantially perpendicular to the face side, wherein the wedge side includes a wedge angle of two degrees plus or minus one tenth of a degree; and a major side that is substantially perpendicular to the face side and the wedge side. The major side includes a thickness of one and one-half millimeter plus or minus one tenth of a millimeter. A polarized light beam entering the birefringent crystal at an incident angle is separated into an ordinary light beam and an extraordinary light beam.
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公开(公告)号：US20220082489A1
公开(公告)日：2022-03-17
申请号：US17304844
申请日：2021-06-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huiming Gu , Shao Qing , Ming Yan , David Vrane
IPC: G01N15/14
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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