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    Nucleotide sequences which code for the sahH gene
    1.
    发明申请
    Nucleotide sequences which code for the sahH gene 有权
    编码sahH基因的核苷酸序列

    公开(公告)号：US20030100080A1

    公开(公告)日：2003-05-29

    申请号：US09919854

    申请日：2001-08-02

    申请人： DEGUSSA AG

    发明人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    IPC分类号： C12P013/04 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

    CPC分类号： C12N9/88 ,  A23K10/12 ,  A23K20/142 ,  A23K40/35 ,  A23K50/10 ,  C12P13/08 ,  C12P13/12

    摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.

    摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sahH基因以增强形式存在，以及使用 含有根据本发明的序列的多核苷酸作为杂交探针。

    Nucleotide sequences which code for the metY gene
    2.
    发明申请
    Nucleotide sequences which code for the metY gene 有权
    编码metY基因的核苷酸序列

    公开(公告)号：US20020110878A1

    公开(公告)日：2002-08-15

    申请号：US09919932

    申请日：2001-08-02

    申请人： DEGUSSA AG

    发明人： Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    IPC分类号： C12P013/08 ,  C12N009/10 ,  C07H021/04 ,  C12N015/74 ,  C12P021/02 ,  C12N001/21

    CPC分类号： C12P13/12 ,  C12N9/88 ,  C12P13/08

    摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metY gene is present in enhanced form, and use of the polynucleotides as hybridization probes.

    摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metY基因以增强形式存在，以及使用多核苷酸 作为杂交探针。

    Process for the preparation of unsaturated ketones
    4.
    发明申请
    Process for the preparation of unsaturated ketones 失效
    制备不饱和酮的方法

    公开(公告)号：US20020169342A1

    公开(公告)日：2002-11-14

    申请号：US10132195

    申请日：2002-04-26

    申请人： DEGUSSA AG

    发明人： Nongyuan Shi ,  Bernd Drapal ,  Steffen Krill ,  Markus Julino ,  Klaus Huthmacher

    IPC分类号： C07C045/68

    CPC分类号： C07C45/513 ,  C07C45/62 ,  C07C49/203 ,  C07C49/04

    摘要： A process for the preparation of null,null,null-unsaturated ketones and/or null,null,null,null-unsaturated ketones by the reaction of an unsaturated alcohol with an enol ether or mixture of enol ethers, with formation of ketals as a by-product, at temperatures of from 50null C. to 200null C., in the presence of an acid catalyst, whereby one or all of the reagents is/are heated to the reaction temperature of from 50null C. to 200null C. before the acid catalyst is added.

    摘要翻译： 通过不饱和醇与烯醇醚或烯醇醚的混合物的反应制备β，γ，δ-不饱和酮和/或α，β，γ，δ-不饱和酮的方法，形成缩酮作为 副产物，在50℃至200℃的温度下，在酸催化剂存在下，由此将一种或全部试剂加热至50℃至200℃的反应温度 在添加酸催化剂之前。

    Nucleotide sequences which code for the metE gene
    5.
    发明申请
    Nucleotide sequences which code for the metE gene 有权
    编码metE基因的核苷酸序列

    公开(公告)号：US20020110877A1

    公开(公告)日：2002-08-15

    申请号：US09919835

    申请日：2001-08-02

    申请人： DEGUSSA AG

    发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    IPC分类号： C12P013/12 ,  C12P013/04 ,  A61K031/70

    CPC分类号： C12N9/1007 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

    摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因以增强形式存在，以及使用多核苷酸 序列作为杂交探针。

    Nucleotide sequence which code for the metH gene
    6.
    发明申请
    Nucleotide sequence which code for the metH gene 有权
    编码metH基因的核苷酸序列

    公开(公告)号：US20020048793A1

    公开(公告)日：2002-04-25

    申请号：US09919891

    申请日：2001-08-02

    申请人： DEGUSSA AG

    发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

    IPC分类号： C12P021/02 ,  C07H021/04 ,  C12N001/21 ,  C12N015/74

    CPC分类号： C12N9/1007 ,  A23K20/142 ,  C12P13/12 ,  C12R1/15

    摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.

    摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metH基因以增强形式存在，以及使用多核苷酸序列 作为杂交探针。

    Process for the preparation of ketones
    7.
    发明申请
    Process for the preparation of ketones 审中-公开
    酮的制备方法

    公开(公告)号：US20020161263A1

    公开(公告)日：2002-10-31

    申请号：US10132196

    申请日：2002-04-26

    申请人： DEGUSSA AG

    发明人： Nongyuan Shi ,  Steffen Krill ,  Klaus Huthmacher ,  Bernd Drapal

    IPC分类号： C07C045/68

    CPC分类号： C07C45/62 ,  C07C45/513 ,  C07C49/203 ,  C07C49/04

    摘要： A process for the preparation of ketones corresponding to the general Formula I 1 in which null,null,null-allenic, di-unsaturated ketones and/or null,null,null,null-conjugated, di-unsaturated ketones, obtained by reaction of propargyl alcohols with enol ethers and are converted in direct manner by hydrogenation to the saturated ketones corresponding to the general Formula I without purification by distillation.

    摘要翻译： 制备相应于通式I的酮的方法，其中通过反应得到的β，γ，δ-泛酸，二不饱和酮和/或α，β，γ，δ-缀合的二不饱和酮， 具有烯醇醚的醇，并且通过氢化直接转化成对应于通式I的饱和酮而不通过蒸馏纯化。

    Nucleotide sequences which code for the metD gene
    10.
    发明申请
    Nucleotide sequences which code for the metD gene 审中-公开
    编码metD基因的核苷酸序列

    公开(公告)号：US20030092026A1

    公开(公告)日：2003-05-15

    申请号：US10156856

    申请日：2002-05-30

    申请人： DEGUSSA AG

    发明人： Daniel Rey ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Brigitte Bathe ,  Klaus Huthmacher ,  Walter Pfefferle

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21

    CPC分类号： C07K14/34 ,  A23K10/12 ,  A23K20/142 ,  A23K40/10 ,  C12P13/12

    摘要： The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the metD gene and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of MetD transcription regulator activity, methods for preparation of L-amino acids, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which comprise varying degrees of MetD transcription regulator activity. Further, the invention relates to animal food additives based on fermentation liquor and containing L-methionine, and to the preparation of such additive.

    摘要翻译： 本发明涉及多核苷酸，其包含对应于metD基因的多核苷酸序列及其部分，其编码具有不同程度的MetD转录调节活性的多肽序列及其部分，用于制备L-氨基酸的方法，以及筛选和扩增编码多肽的多核苷酸的方法 包含不同程度的MetD转录调节活性的序列。 此外，本发明涉及基于发酵液并含有L-甲硫氨酸的动物性食品添加剂以及这种添加剂的制备。