公开(公告)号：US20020173012A1

公开(公告)日：2002-11-21

申请号：US10142876

申请日：2002-05-13

发明人： Marco Berna ,  Lino Sivieri ,  Gianni Santambrogio ,  Ermanno Valoti

IPC分类号： C12P013/12

CPC分类号： C07H1/08 ,  C07H19/16 ,  C12P19/40

摘要： The present invention relates to a process for the preparation of pharmaceutically acceptable salts of (SS,RS)-S-adenosyl-L-methionine and allows to obtain the salified (RS)-(null)-S-adenosyl-L-methionine diastereoisomer in amounts lower than or equal to 3% with respect to the salified (SS)-(null)-S-adenosyl-L-methionine diastereoisomer; the salts that can be obtained by the process of the invention keep their configuration stable in time.

摘要翻译： 本发明涉及制备（SS，RS）-S-腺苷-L-甲硫氨酸的药学上可接受的盐的方法，并且可以获得盐化的（RS） - （+） - S-腺苷-L-甲硫氨酸非对映异构体 相对于成盐（SS） - （+） - S-腺苷-L-甲硫氨酸非对映异构体而言，其含量低于或等于3％ 可以通过本发明的方法获得的盐保持其构型在时间上稳定。

公开(公告)号：US20030186393A1

公开(公告)日：2003-10-02

申请号：US10254763

申请日：2002-09-26

申请人： Ajinomoto Co., Inc.

发明人： Hiroshi Takagi ,  Masaru Wada ,  Shigeru Nakamori

IPC分类号： C12P013/12 ,  C12N009/10 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

CPC分类号： C12R1/19 ,  C12N9/88 ,  C12P13/12

摘要： L-Cysteine is produced by culturing a bacterium belonging to the genus Escherichia having an L-cysteine producing ability and modified so that cystathionine-null-lyase activity or cystathionine-null-lyase activity and tryptophanase activity should be reduced or eliminated in a medium to produce and accumulate L-cysteine in the medium and collecting the L-cysteine from the medium.

摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏杆菌属细菌产生L-半胱氨酸，并进行修饰，以便在培养基中减少或消除胱硫醚-β-裂解酶活性和色氨酸酶活性 在培养基中产生并积累L-半胱氨酸，并从培养基中收集L-半胱氨酸。

公开(公告)号：US20030166174A1

公开(公告)日：2003-09-04

申请号：US10309089

申请日：2002-12-04

申请人： Ajinomoto Co., Inc.

发明人： Yukiko Ono ,  Hisashi Yasueda ,  Yoshio Kawahara ,  Shinichi Sugimoto

IPC分类号： C12P013/04 ,  C12P013/14 ,  C12P013/12 ,  C12P013/08 ,  C12P013/06

CPC分类号： C12R1/26 ,  C12P13/04 ,  C12P13/08 ,  C12P13/12 ,  C12R1/01

摘要： A methane-utilizing microorganism capable of producing L-amino acid, for example, bacteria belonging to type I, type X or type II in the taxonomic categorization methane-utilizing bacteria such as Methylomonas albus, Methylococcus capsulatus and Methylosinus trichosporium, is cultivated in a culture medium in contact with gas containing methane which is the main source of carbon, to allow the L-amino acid to be produced and accumulated in the medium, and the L-amino acid is collected from the medium.

摘要翻译： 能够生产L-氨基酸的甲烷利用微生物，例如属于I型，X型或II型的细菌，在分类分类甲烷利用细菌如Methylomonas albus，Methylococcus capsulatus和Methylosinus trichosporium中培养在 与作为主要碳源的甲烷的气体接触的培养基，以使L-氨基酸在培养基中产生和积累，从培养基中收集L-氨基酸。

公开(公告)号：US20030077766A1

公开(公告)日：2003-04-24

申请号：US10060218

申请日：2002-02-01

申请人： AJINOMOTO CO. INC.

发明人： Hiroshi Takagi ,  Masaru Wada ,  Shigeru Nakamori

IPC分类号： C12P013/04 ,  C12P013/12 ,  C12N009/10 ,  C12P021/02 ,  C12N001/21

CPC分类号： C12P13/12

摘要： L-cysteine is produced by culturing a coryneform bacterium in which intracellular serine acetyltransferase activity is increased by transformation with a gene coding for serine acetyltransferase, such modification of an expression control sequence of a gene coding for serine acetyltransferase that expression of the gene in a cell should be enhanced and for forth, preferably in which L-cysteine decomposition system is further suppressed, in a medium to produce and accumulate L-cysteine in culture and collecting the L-cysteine from the culture.

摘要翻译： 通过培养通过用编码丝氨酸乙酰转移酶的基因进行转化而增加细胞内丝氨酸乙酰转移酶活性的棒状细菌来产生L-半胱氨酸，这种修饰编码丝氨酸乙酰转移酶的基因的表达控制序列，该基因在细胞中的表达 应在培养基中进一步增强，优选在其中进一步抑制L-半胱氨酸分解系统，以在培养物中产生和积累L-半胱氨酸并从培养物中收集L-半胱氨酸。

公开(公告)号：US20020110877A1

公开(公告)日：2002-08-15

申请号：US09919835

申请日：2001-08-02

申请人： DEGUSSA AG

发明人： Brigitte Bathe ,  Bettina Moeckel ,  Walter Pfefferle ,  Klaus Huthmacher ,  Christian Rueckert ,  Joern Kalinowski ,  Alfred Puehler ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12P013/12 ,  C12P013/04 ,  A61K031/70

CPC分类号： C12N9/1007 ,  A23K20/142 ,  C07K14/34 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）至少包含至少 a），b）或c）的多核苷酸序列的15个连续核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少metE基因以增强形式存在，以及使用多核苷酸 序列作为杂交探针。