公开(公告)号：US20240147996A1

公开(公告)日：2024-05-09

申请号：US18504940

申请日：2023-11-08

Applicant: DOW AGROSCIENCES LLC ,  M.S. TECHNOLOGIES, L.L.C.

Inventor： Yunxing C. CUI ,  Thomas HOFFMAN ,  Ning ZHOU ,  Stephen N. NOVAK ,  Julissa COLON ,  Dawn M. PARKHURST ,  Sandra G. TOLEDO ,  Terry R. WRIGHT ,  Sean M. RUSSELL ,  Bruce HELD ,  Vaithilingam SEKAR

IPC: A01N39/04 ,  C12N9/00 ,  C12N9/10 ,  C12N15/82 ,  C12Q1/6895

CPC classification number: A01N39/04 ,  C12N9/1092 ,  C12N9/93 ,  C12N15/8241 ,  C12N15/8274 ,  C12N15/8275 ,  C12Q1/6895 ,  C12Y205/01019 ,  C12Y604/01002 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

公开(公告)号：US20250154492A1

公开(公告)日：2025-05-15

申请号：US19029959

申请日：2025-01-17

Applicant: CORTEVA AGRISCIENCE LLC ,  M.S. TECHNOLOGIES, L.L.C.

Inventor： Yunxing C. CUI ,  Thomas HOFFMAN ,  Ning ZHOU ,  Stephen N. NOVAK ,  Julissa COLON ,  Dawn M. PARKHURST ,  Sandra G. TOLEDO ,  Terry R. WRIGHT ,  Sean M. RUSSELL ,  Bruce HELD ,  Vaithilingam SEKAR

IPC: C12N9/00 ,  A01N39/04 ,  C12N9/10 ,  C12N15/82 ,  C12Q1/6895

Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays arc also provided.

公开(公告)号：US20210403934A1

公开(公告)日：2021-12-30

申请号：US17386891

申请日：2021-07-28

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing Cory CUI ,  Jill BRYAN ,  Donald MAUM ,  Greg GILLES ,  Terry WRIGHT ,  Jennifer HAMILTON ,  Nicole ARNOLD ,  Nathan VanOpdorp ,  Tina KAISER ,  Ning ZHOU

IPC: C12N15/82 ,  C12Q1/6895 ,  C12N9/02

Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel AAD-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (or corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US20190233904A1

公开(公告)日：2019-08-01

申请号：US16279055

申请日：2019-02-19

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing Cory CUI ,  Jill BRYAN ,  Donald MAUM ,  Greg GILLES ,  Terry WRIGHT ,  Jennifer HAMILTON ,  Nicole ARNOLD ,  Nathan VanOpdorp ,  Tina KAISER ,  Ning ZHOU

IPC: C12Q1/6895 ,  C12N15/82 ,  A01H5/10

CPC classification number: C12Q1/6895 ,  A01H5/10 ,  C12N9/0069 ,  C12N9/0071 ,  C12N15/8274 ,  C12Q2600/16 ,  C12Y113/00

Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (or corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

公开(公告)号：US20170044558A1

公开(公告)日：2017-02-16

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David R. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82 ,  C12N9/12

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。

公开(公告)号：US20140173783A1

公开(公告)日：2014-06-19

申请号：US14105563

申请日：2013-12-13

Applicant: Dow AgroSciences LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David H. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32中的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。