公开(公告)号：US20210095325A1

公开(公告)日：2021-04-01

申请号：US17050639

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Otto Martin LEHMANN ,  Harald PICHLER ,  Holly STOLTERFOHT

IPC: C12P33/00 ,  C12N9/10 ,  C12N9/02

Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a host strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20170044556A1

公开(公告)日：2017-02-16

申请号：US15306120

申请日：2015-04-28

Applicant: DSM IP ASSETS B.V.

Inventor： Anita EMMERSTORFER ,  Tamara WRIESSNEGGER ,  Miriam WIMMER-TEUBENBACHER ,  Harald PICHLER ,  Monika MUELLER ,  Iwona KALUZNA ,  Martin SCHURMANN

IPC: C12N15/81 ,  C12N9/02 ,  C07K14/395 ,  C12P7/26

CPC classification number: C12N15/81 ,  C07K14/395 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0073 ,  C12P7/02 ,  C12P7/26 ,  C12P19/56 ,  C12P33/00 ,  C12Y106/02004 ,  C12Y114/13121

Abstract: The present invention relates to a recombinant microorganism which is capable of expressing a cytochrome P450 reductase (CPR) and, optionally, a cytochrome P450 enzyme (CYP) and which is capable of overexpressing a Ice2p comprising the amino acid sequence set out in SEQ ID NO: 2 or a sequence having at least 50% sequence identity thereto. The invention further relates to use of Ice2p to stabilize expression of a cytochrome P450 reductase in a recombinant microorganism.

Abstract translation: 本发明涉及能够表达细胞色素P450还原酶（CPR）和任选的细胞色素P450酶（CYP）的重组微生物，其能够过表达包含SEQ ID NO中所示的氨基酸序列的Ice2p ：2或与其具有至少50％序列同一性的序列。 本发明还涉及使用Ice2p来稳定重组微生物中细胞色素P450还原酶的表达。

公开(公告)号：US20210222144A1

公开(公告)日：2021-07-22

申请号：US15734370

申请日：2019-06-04

Applicant: DSM IP Assets B.V.

Inventor： Harald PICHLER ,  Martin SCHÜRMANN ,  Rolf SCHUETZ ,  Matthias ENGLEDER ,  Gernot STROHMEIER

IPC: C12N9/88 ,  C11C3/00

Abstract: The present invention relates to regio- and stereoselective hydration of oleic acid derivatives, including esters of oleic acid, by action of modified oleate hydratase.

公开(公告)号：US20210180103A1

公开(公告)日：2021-06-17

申请号：US16065274

申请日：2016-12-20

Applicant: DSM IP ASSETS B.V.

Inventor： Hans-Peter HOHMANN ,  Regina LEBER ,  Martin LEHMANN ,  Corinna ODAR ,  Barbara PETSCHACHER ,  Harald PICHLER ,  Birgit PLOIER

IPC: C12P33/16 ,  C12N9/02 ,  C12N9/10 ,  C12N1/16

Abstract: The present invention is related to production of a sterol mix in a modified yeast cell, wherein the amount of zymosterol present in said mix is dramatically reduced or abolished via modification of sterol acyltransferase activity within said yeast. The modified yeast cell can be used for production of vitamin D3 or derivatives and/or metabolites thereof.