公开(公告)号：US5726127A

公开(公告)日：1998-03-10

申请号：US646352

申请日：1996-07-24

申请人： Dae Whang Kim ,  Kyeong Yeol Yun ,  Jae Wook Ryu ,  Yeon Soo Lee ,  Seung Kyu Kang ,  In Taek Hwang

发明人： Dae Whang Kim ,  Kyeong Yeol Yun ,  Jae Wook Ryu ,  Yeon Soo Lee ,  Seung Kyu Kang ,  In Taek Hwang

IPC分类号： C07D409/12 ,  A01N47/36 ,  C07D333/34 ,  C07D491/048 ,  C07D491/052 ,  C07D521/00

CPC分类号： C07D521/00 ,  A01N47/36 ,  C07D333/34

摘要： The present invention relates to a thiophenesulfonylurea derivative represented by general formula (I), the process for preparation thereof, and herbicide using it; and a salt thereof, in which T represents a group T1, T2, or T3 having general formulae (a), wherein E represents a direct bond; R represents hydrogen or C.sub.1-4 acyl; R.sup.1 represents C.sub.1-6 alkyl substituted with 1 to 3 halogen atoms; R.sup.2 represents hydrogen, C.sub.1-3 alkyl, C.sub.1-3 haloalkyl, halogen, cyano, C.sub.1-3 alkoxy, C.sub.1-3 haloalkoxy, amino, methylamino, dimethylamino, or C.sub.1-3 alkyl substituted with C.sub.1-2 alkoxy, C.sub.1-2 haloalkoxy, thiol, methylthio, cyano or hydroxy; A represents a group selected from A1 to A4 having general formulae (b), and a herbicidal composition containing these derivatives.

摘要翻译： PCT No.PCT / KR94 / 00161 Sec。 371日期：1996年7月24日 102（e）日期1996年7月24日PCT 1994年11月9日PCT PCT。 公开号WO95 / 13276 日期1995年5月18日本发明涉及由通式（I）表示的噻吩磺酰脲衍生物，其制备方法和使用它的除草剂; 和其盐，其中T表示具有通式（a）的基团T1，T2或T3，其中E表示直接键; R代表氢或C1-4酰基; R 1表示被1至3个卤素原子取代的C 1-6烷基; R 2表示氢，C 1-3烷基，C 1-3卤代烷基，卤素，氰基，C 1-3烷氧基，C 1-3卤代烷氧基，氨基，甲基氨基，二甲基氨基或被C 1-2烷氧基取代的C 1-3烷基， ，硫醇，甲硫基，氰基或羟基; A表示选自具有通式（b）的A1至A4的基团和含有这些衍生物的除草组合物。

公开(公告)号：US08956842B2

公开(公告)日：2015-02-17

申请号：US14111647

申请日：2011-08-12

申请人： In Taek Hwang ,  No Joong Park ,  He Kyoung Lim

发明人： In Taek Hwang ,  No Joong Park ,  He Kyoung Lim

IPC分类号： C12N9/12 ,  C12N15/00 ,  C12N1/20 ,  C07K1/00 ,  C12N9/24 ,  A23K1/165 ,  A23K1/18

CPC分类号： C12N9/2482 ,  A23K20/189 ,  A23K50/10 ,  A23K50/75 ,  C12N9/248 ,  C12Y302/01008

摘要： The present invention relates to the novel Paenibacillus sp. strain, and the novel protein isolated from the same. More particularly, the present invention relates to the novel Paenibacillus sp. strain producing xylanase, and the novel xylanase having high activity at high temperature and in a wide range of pH, and a production method of the same. The Paenibacillus sp. HPL-3 strain (KCTC11987BP) and the xylanase of the present invention demonstrates high activity at high temperature or in a wide range of pH to decompose xylan, the major component of various lignocellulosic biomass, so that they can be effectively used for the production or development of bio-fuel, alternative material, performance chemical, bio-polymer, food and feeds, etc.

摘要翻译： 本发明涉及新型Paenibacillus sp。 菌株和从其分离的新型蛋白质。 更具体地说，本发明涉及新型的Paenibacillus sp。 菌株生产木聚糖酶，以及在高温和pH范围内具有高活性的新型木聚糖酶及其制备方法。 Paenibacillus sp。 HPL-3菌株（KCTC11987BP）和本发明的木聚糖酶在高温或宽范围的pH下表现出高活性，以分解各种木质纤维素生物质的主要组分木聚糖，从而可以有效地用于生产或 开发生物燃料，替代材料，性能化学，生物聚合物，食品和饲料等。

公开(公告)号：US09328377B2

公开(公告)日：2016-05-03

申请号：US13508086

申请日：2009-11-28

申请人： Jong Yoon Chun ,  In Taek Hwang ,  Young Jo Lee

发明人： Jong Yoon Chun ,  In Taek Hwang ,  Young Jo Lee

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6823 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q2565/1015 ,  C12Q2561/113 ,  C12Q2561/101

摘要： The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection primer (THD primer). The present invention allows for both a target amplification and a signal amplification by introducing a label into a primer used in PCR reactions, ensuring a real-time target detection by PCR reaction by no use of complicated oligonucleotides. The present invention could completely be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. This feature makes it possible that the present invention exhibits excellent real-time target detection in multiplex manner.

摘要翻译： 本发明涉及使用靶杂交和检测引物（THD引物）检测靶核酸序列。 本发明通过在PCR反应中使用的引物中引入标记物来允许目标扩增和信号扩增两者，通过不使用复杂的寡核苷酸来确保PCR反应的实时目标检测。 本发明可以完全免除与常规实时PCR方法相关的麻烦的问题和缺点。 本发明允许仅使用标记的引物成功实现目标检测。 该特征使得本发明可以以多路复用方式显示出优异的实时目标检测。

公开(公告)号：US20140038262A1

公开(公告)日：2014-02-06

申请号：US14111647

申请日：2011-08-12

申请人： In Taek Hwang ,  No Joong Park ,  He Kyoung Lim

发明人： In Taek Hwang ,  No Joong Park ,  He Kyoung Lim

IPC分类号： C12N9/24

CPC分类号： C12N9/2482 ,  A23K20/189 ,  A23K50/10 ,  A23K50/75 ,  C12N9/248 ,  C12Y302/01008

摘要： The present invention relates to the novel Paenibacillus sp. strain, and the novel protein isolated from the same. More particularly, the present invention relates to the novel Paenibacillus sp. strain producing xylanase, and the novel xylanase having high activity at high temperature and in a wide range of pH, and a production method of the same. The Paenibacillus sp. HPL-3 strain (KCTC11987BP) and the xylanase of the present invention demonstrates high activity at high temperature or in a wide range of pH to decompose xylan, the major component of various lignocellulosic biomass, so that they can be effectively used for the production or development of bio-fuel, alternative material, performance chemical, bio-polymer, food and feeds, etc.

摘要翻译： 本发明涉及新型Paenibacillus sp。 菌株和从其分离的新型蛋白质。 更具体地说，本发明涉及新型的Paenibacillus sp。 菌株生产木聚糖酶，以及在高温和pH范围内具有高活性的新型木聚糖酶及其制备方法。 Paenibacillus sp。 HPL-3菌株（KCTC11987BP）和本发明的木聚糖酶在高温或宽范围的pH下表现出高活性，以分解各种木质纤维素生物质的主要组分木聚糖，从而可以有效地用于生产或 开发生物燃料，替代材料，性能化学，生物聚合物，食品和饲料等。

公开(公告)号：US20120264643A1

公开(公告)日：2012-10-18

申请号：US13515975

申请日：2010-03-26

申请人： Jong Yoon Chun ,  In Taek Hwang

发明人： Jong Yoon Chun ,  In Taek Hwang

IPC分类号： C12Q1/68 ,  C40B30/04 ,  C40B40/06 ,  G01N21/64

CPC分类号： C12Q1/6816 ,  C12Q2565/107 ,  C12Q2565/1015 ,  C12Q2565/137

摘要： The present invention relates to the detection of a target nucleic acid sequence in a real-time manner using a target signal generating primer (TSG primer) having dual interactive labels. The present invention allows for both target amplification and signal amplification by introducing dual interactive labels into a primer used in PCR reactions, ensuring real-time target detection by PCR reactions without the use of complicated oligonucleotides. The present invention could be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. Also, the present invention can obtain strong signals indicative of the presence of target nucleic acid sequences in both a liquid phase and solid phase.

摘要翻译： 本发明涉及使用具有双重交互标签的目标信号产生引物（TSG引物）以实时方式检测靶核酸序列。 本发明允许通过将双重交互标记引入PCR反应中使用的引物来进行靶扩增和信号扩增，确保通过PCR反应的实时目标检测而不使用复杂的寡核苷酸。 本发明可以免于与常规实时PCR方法相关的麻烦的事项和缺点。 本发明允许仅使用标记的引物成功实现目标检测。 此外，本发明可以获得指示靶核酸序列在液相和固相中存在的强信号。

公开(公告)号：US20120220468A1

公开(公告)日：2012-08-30

申请号：US13392400

申请日：2010-09-02

申请人： Jong Yoon Chun ,  In Taek Hwang ,  Sang Kil Lee

发明人： Jong Yoon Chun ,  In Taek Hwang ,  Sang Kil Lee

IPC分类号： C40B20/04 ,  C07H21/04 ,  C07H1/00 ,  G01N21/64

CPC分类号： C07H21/00 ,  C12Q1/6823 ,  C12Q2565/1015 ,  C12Q2561/101 ,  C12Q2525/161

摘要： The present invention relates to a target discriminative probe (TD probe) and its uses or applications. The TD probe is hybridized with a target nucleic acid sequence through both of the 5′-second hybridization portion and the 3′-first hybridization portion. When the TD probe is hybridized with a non-target nucleic acid sequence, both the 5′-second hybridization portion and the separation portion are not hybridized with the non-target nucleic acid sequence such that both portions form a single strand due to its low Tm value. As such, the TD probe exhibits distinctly different hybridization patterns for each of the target and the non-target nucleic acid sequence, discriminating the target nucleic acid sequence from the non-target nucleic acid sequence with much higher specificity.

摘要翻译： 本发明涉及目标鉴别探针（TD探针）及其用途或应用。 TD探针通过5'-第二杂交部分和3'-第一杂交部分与靶核酸序列杂交。 当TD探针与非靶核酸序列杂交时，5'-第二杂交部分和分离部分都不与非靶核酸序列杂交，使得两部分由于其低部分形成单链 Tm值。 因此，TD探针对靶和非靶核酸序列中的每一个显示明显不同的杂交模式，以非常高的特异性区分靶核酸序列与非靶核酸序列。

公开(公告)号：US20120219955A1

公开(公告)日：2012-08-30

申请号：US13508086

申请日：2009-11-28

申请人： Jong Yoon Chun ,  In Taek Hwang ,  Young Jo Lee

发明人： Jong Yoon Chun ,  In Taek Hwang ,  Young Jo Lee

IPC分类号： G01N21/64

CPC分类号： C12Q1/6823 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q2565/1015 ,  C12Q2561/113 ,  C12Q2561/101

摘要： The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection primer (THD primer). The present invention allows for both a target amplification and a signal amplification by introducing a label into a primer used in PCR reactions, ensuring a real-time target detection by PCR reaction by no use of complicated oligonucleotides. The present invention could completely be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. This feature makes it possible that the present invention exhibits excellent real-time target detection in multiplex manner.

摘要翻译： 本发明涉及使用靶杂交和检测引物（THD引物）检测靶核酸序列。 本发明通过在PCR反应中使用的引物中引入标记物来允许目标扩增和信号扩增两者，通过不使用复杂的寡核苷酸来确保PCR反应的实时目标检测。 本发明可以完全免除与常规实时PCR方法相关的麻烦的问题和缺点。 本发明允许仅使用标记的引物成功实现目标检测。 该特征使得本发明可以以多路复用方式显示出优异的实时目标检测。

公开(公告)号：US20120190030A1

公开(公告)日：2012-07-26

申请号：US13497310

申请日：2010-04-09

申请人： Jong Yoon Chun ,  In Taek Hwang

发明人： Jong Yoon Chun ,  In Taek Hwang

IPC分类号： G01N21/64

CPC分类号： C12Q1/682 ,  C12Q2565/1025 ,  C12Q2521/319

摘要： The present invention relates to the detection of a target nucleic acid sequence by a cyclic exonucleolytic reaction. The present method enabling to generate signals by probe digestion with no help of primers and to amplify signals with no help of simultaneous target amplification reactions may enable to detect multiple target sequences without any problems accounted in the conventional real-time PCR methods such as false positive signals and difficulties in oligonucleotides (primer and probe) selection and reaction condition optimization.

摘要翻译： 本发明涉及通过循环核酸内切酶反应检测靶核酸序列。 通过探针消化而不用引物帮助产生信号并且在没有同时靶扩增反应的帮助下扩增信号的本发明方法可以使得能够检测多个靶序列，而不存在常规实时PCR方法中所存在的任何问题，例如假阳性 寡核苷酸（引物和探针）选择和反应条件优化的信号和困难。

公开(公告)号：US09845492B2

公开(公告)日：2017-12-19

申请号：US13515975

申请日：2010-03-26

申请人： Jong Yoon Chun ,  In Taek Hwang

发明人： Jong Yoon Chun ,  In Taek Hwang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q2565/107 ,  C12Q2565/1015 ,  C12Q2565/137

摘要： The present invention relates to the detection of a target nucleic acid sequence in a real-time manner using a target signal generating primer (TSG primer) having dual interactive labels. The present invention allows for both target amplification and signal amplification by introducing dual interactive labels into a primer used in PCR reactions, ensuring real-time target detection by PCR reactions without the use of complicated oligonucleotides. The present invention could be free from the troublesome matters and shortcomings associated with conventional real-time PCR methods. The present invention allows for successful real-time target detection by using only a labeled primer. Also, the present invention can obtain strong signals indicative of the presence of target nucleic acid sequences in both a liquid phase and solid phase.

公开(公告)号：US09447457B2

公开(公告)日：2016-09-20

申请号：US13497310

申请日：2010-04-09

申请人： Jong Yoon Chun ,  In Taek Hwang

发明人： Jong Yoon Chun ,  In Taek Hwang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/682 ,  C12Q2565/1025 ,  C12Q2521/319

摘要： The present invention relates to the detection of a target nucleic acid sequence by a cyclic exonucleolytic reaction. The present method enabling to generate signals by probe digestion with no help of primers and to amplify signals with no help of simultaneous target amplification reactions may enable to detect multiple target sequences without any problems accounted in the conventional real-time PCR methods such as false positive signals and difficulties in oligonucleotides (primer and probe) selection and reaction condition optimization.

摘要翻译： 本发明涉及通过循环核酸内切酶反应检测靶核酸序列。 通过探针消化而不用引物帮助产生信号并且在没有同时靶扩增反应的帮助下扩增信号的本发明方法可以使得能够检测多个靶序列，而不存在常规实时PCR方法中所存在的任何问题，例如假阳性 寡核苷酸（引物和探针）选择和反应条件优化的信号和困难。