公开(公告)号：US07785811B2

公开(公告)日：2010-08-31

申请号：US11061341

申请日：2005-02-18

申请人： Daniel E. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Aya Jakobovits

发明人： Daniel E. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Aya Jakobovits

IPC分类号： G01N33/574

CPC分类号： C07K14/47 ,  A61K39/00 ,  A61K48/00 ,  C07K2317/24 ,  C07K2319/02 ,  C12N2799/026 ,  C12N2799/027

摘要： Described is a novel gene and its encoded secreted tumor antigen, termed BPC-1, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers which express BPC-1, particularly including prostate cancer and bladder cancer. In human normal tissues, BPC-1 is only expressed in certain tissues of the brain. However, BPC-1 is expressed at high levels in prostate cancer cells and is also expressed in bladder cancer cells. The structure of BPC-1 includes a signal sequence and a CUB domain. BPC-1 protein is secreted. Preliminary experimental evidence suggests that BPC-1 is directly involved in oncogenesis or maintenance of the transformed phenotype of cancer cells expressing BPC-1. BPC-1 also appears to bind specifically to a cellular protein expressed in prostate cancer cells and other cells.

摘要翻译： 描述了一种新型基因及其编码的分泌的肿瘤抗原，称为BPC-1，以及用于治疗表达BPC-1的各种癌症的诊断和治疗方法和组合物，特别包括前列腺癌和膀胱癌。 在人正常组织中，BPC-1仅在脑的某些组织中表达。 然而，BPC-1在前列腺癌细胞中以高水平表达，并且也在膀胱癌细胞中表达。 BPC-1的结构包括信号序列和CUB域。 分泌BPC-1蛋白。 初步实验证据表明，BPC-1直接参与肿瘤发生或维持表达BPC-1的癌细胞的转化表型。 BPC-1也似乎特异性结合前列腺癌细胞和其他细胞中表达的细胞蛋白。

公开(公告)号：US20100168027A1

公开(公告)日：2010-07-01

申请号：US12718821

申请日：2010-03-05

申请人： Daniel E. AFAR ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas C. Saffran ,  Aya Jakobovits

发明人： Daniel E. AFAR ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas C. Saffran ,  Aya Jakobovits

IPC分类号： A61K38/17 ,  C07K14/435 ,  C07K16/18 ,  C12N5/16

CPC分类号： C07K14/47 ,  A61K39/00 ,  A61K48/00 ,  C07K2317/24 ,  C07K2319/02 ,  C12N2799/026 ,  C12N2799/027

摘要： Described is a novel gene and its encoded secreted tumor antigen, termed BPC-1, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers which express BPC-1, particularly including prostate cancer and bladder cancer. In human normal tissues, BPC-1 is only expressed in certain tissues of the brain. However, BPC-1 is expressed at high levels in prostate cancer cells and is also expressed in bladder cancer cells. The structure of BPC-1 includes a signal sequence and a CUB domain. BPC-1 protein is secreted. Preliminary experimental evidence suggests that BPC-1 is directly involved in oncogenesis or maintenance of the transformed phenotype of cancer cells expressing BPC-1. BPC-1 also appears to bind specifically to a cellular protein expressed in prostate cancer cells and other cells.

摘要翻译： 描述了一种新型基因及其编码的分泌的肿瘤抗原，称为BPC-1，以及用于治疗表达BPC-1的各种癌症的诊断和治疗方法和组合物，特别包括前列腺癌和膀胱癌。 在人正常组织中，BPC-1仅在脑的某些组织中表达。 然而，BPC-1在前列腺癌细胞中以高水平表达，并且也在膀胱癌细胞中表达。 BPC-1的结构包括信号序列和CUB域。 分泌BPC-1蛋白。 初步实验证据表明，BPC-1直接参与肿瘤发生或维持表达BPC-1的癌细胞的转化表型。 BPC-1也似乎特异性结合前列腺癌细胞和其他细胞中表达的细胞蛋白。

公开(公告)号：US08003758B2

公开(公告)日：2011-08-23

申请号：US12718821

申请日：2010-03-05

申请人： Daniel E. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas C. Saffran ,  Aya Jakobovits

发明人： Daniel E. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas C. Saffran ,  Aya Jakobovits

IPC分类号： C07K5/10

CPC分类号： C07K14/47 ,  A61K39/00 ,  A61K48/00 ,  C07K2317/24 ,  C07K2319/02 ,  C12N2799/026 ,  C12N2799/027

摘要： Described is a novel gene and its encoded secreted tumor antigen, termed BPC-1, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers which express BPC-1, particularly including prostate cancer and bladder cancer. In human normal tissues, BPC-1 is only expressed in certain tissues of the brain. However, BPC-1 is expressed at high levels in prostate cancer cells and is also expressed in bladder cancer cells. The structure of BPC-1 includes a signal sequence and a CUB domain. BPC-1 protein is secreted. Preliminary experimental evidence suggests that BPC-1 is directly involved in oncogenesis or maintenance of the transformed phenotype of cancer cells expressing BPC-1. BPC-1 also appears to bind specifically to a cellular protein expressed in prostate cancer cells and other cells.

摘要翻译： 描述了一种新型基因及其编码的分泌的肿瘤抗原，称为BPC-1，以及用于治疗表达BPC-1的各种癌症的诊断和治疗方法和组合物，特别包括前列腺癌和膀胱癌。 在人正常组织中，BPC-1仅在脑的某些组织中表达。 然而，BPC-1在前列腺癌细胞中以高水平表达，并且也在膀胱癌细胞中表达。 BPC-1的结构包括信号序列和CUB域。 分泌BPC-1蛋白。 初步实验证据表明，BPC-1直接参与肿瘤发生或维持表达BPC-1的癌细胞的转化表型。 BPC-1也似乎特异性结合前列腺癌细胞和其他细胞中表达的细胞蛋白。

公开(公告)号：US06277972B1

公开(公告)日：2001-08-21

申请号：US09374135

申请日：1999-08-10

申请人： Daniel E. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas C. Saffran ,  Aya Jakobovits

发明人： Daniel E. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas C. Saffran ,  Aya Jakobovits

IPC分类号： C07H2102

CPC分类号： C07K14/47 ,  A61K39/00 ,  A61K48/00 ,  C07K2317/24 ,  C07K2319/02 ,  C12N2799/026 ,  C12N2799/027

摘要： Described is a novel gene and its encoded secreted tumor antigen, termed BPC-1, and to diagnostic and therapeutic methods and compositions useful in the management of various cancers which express BPC-1, particularly including prostate cancer and bladder cancer. In human normal tissues, BPC-1 is only expressed in certain tissues of the brain. However, BPC-1 is expressed at high levels in prostate cancer cells and is also expressed in bladder cancer cells. The structure of BPC-1 includes a signal sequence and a CUB domain. BPC-1 protein is secreted. Preliminary experimental evidence suggests that BPC-1 is directly involved in oncogenesis or maintenance of the transformed phenotype of cancer cells expressing BPC-1. BPC-1 also appears to bind specifically to a cellular protein expressed in prostate cancer cells and other cells.

摘要翻译： 描述了一种新型基因及其编码的分泌的肿瘤抗原，称为BPC-1，以及用于治疗表达BPC-1的各种癌症的诊断和治疗方法和组合物，特别包括前列腺癌和膀胱癌。 在人正常组织中，BPC-1仅在脑的某些组织中表达。 然而，BPC-1在前列腺癌细胞中以高水平表达，并且也在膀胱癌细胞中表达。 BPC-1的结构包括信号序列和CUB域。 分泌BPC-1蛋白。 初步实验证据表明，BPC-1直接参与肿瘤发生或维持表达BPC-1的癌细胞的转化表型。 BPC-1也似乎特异性结合前列腺癌细胞和其他细胞中表达的细胞蛋白。

公开(公告)号：US07037667B1

公开(公告)日：2006-05-02

申请号：US09615285

申请日：2000-07-12

申请人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

发明人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

IPC分类号： G01N33/574 ,  G01N33/48 ,  C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/154 ,  G01N33/57419 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/95

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-related, cell membrane associated and secreted serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provided are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. The invention also provides prognostic and diagnostic methods of examining a biological sample for evidence of disregulated cellular growth by comparing the status of 20P1F12/TMPRSS2 in the biological sample to the status of 20P1F12/TMPRSS2 in a corresponding normal sample, wherein alterations in the status of 20P1F12/TMPRSS2 in the biological sample are associated with disregulated cellular growth. The invention further provides various therapeutic compositions and strategies for treating prostate cancer, including particularly, 20P1F12/TMPRSS2 polypeptide and anti-20P1F12/TMPRSS2 antibody therapy methods and compositions, cancer vaccines, and small molecule therapy.

摘要翻译： 描述了源自或基于名为20P1F12 / TMPRSS2的新型前列腺特异性雄激素相关的细胞膜相关和分泌的丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 所提供的组合物是结合20P1F12 / TMPRSS2蛋白和其多肽片段的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本发明还提供了通过将生物样品中20P1F12 / TMPRSS2的状态与对应的正常样品中的20P1F12 / TMPRSS2的状态进行比较来检查生物样品以检测细胞生长失调的证据的预后和诊断方法，其中， 生物样品中的20P1F12 / TMPRSS2与细胞生长失调有关。 本发明还提供了治疗前列腺癌的各种治疗组合物和策略，特别包括20P1F12 / TMPRSS2多肽和抗-20P1F12 / TMPRSS2抗体治疗方法和组合物，癌症疫苗和小分子治疗。

公开(公告)号：US07981624B2

公开(公告)日：2011-07-19

申请号：US12389293

申请日：2009-02-19

申请人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

发明人： Daniel E. H. Afar ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

IPC分类号： G01N33/53

CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/154 ,  G01N33/57419 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/95

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-regulated, cell membrane associated and secreted serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provided are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. The invention also provides prognostic and diagnostic methods of examining a biological sample for evidence of disregulated cellular growth by comparing the status of 20P1F12/TMPRSS2 in the biological sample to the status of 20P1F12/TMPRSS2 in a corresponding normal sample, wherein alterations in the status of 20P1F12/TMPRSS2 in the biological sample are associated with disregulated cellular growth. The invention further provides various therapeutic compositions and strategies for treating prostate cancer, including particularly, 20P1F12/TMPRSS2 polypeptide and anti-20P1F12/TMPRSS2 antibody therapy methods and compositions, cancer vaccines, and small molecule therapy.

摘要翻译： 描述了衍生自或基于称为20P1F12 / TMPRSS2的新型前列腺特异性，雄激素调节的细胞膜相关和分泌的丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 所提供的组合物是结合20P1F12 / TMPRSS2蛋白和其多肽片段的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本发明还提供了通过将生物样品中20P1F12 / TMPRSS2的状态与对应的正常样品中的20P1F12 / TMPRSS2的状态进行比较来检查生物样品以检测细胞生长失调的证据的预后和诊断方法，其中， 生物样品中的20P1F12 / TMPRSS2与细胞生长失调有关。 本发明还提供了治疗前列腺癌的各种治疗组合物和策略，特别包括20P1F12 / TMPRSS2多肽和抗-20P1F12 / TMPRSS2抗体治疗方法和组合物，癌症疫苗和小分子治疗。

公开(公告)号：US20090226921A1

公开(公告)日：2009-09-10

申请号：US12389293

申请日：2009-02-19

申请人： Daniel E. H. AFAR ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

发明人： Daniel E. H. AFAR ,  Rene S. Hubert ,  Kahan Leong ,  Arthur B. Raitano ,  Douglas Saffran ,  Stephen C. Mitchell ,  Aya Jakobovits ,  Mary Faris ,  Igor Vivanco

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/154 ,  G01N33/57419 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/95

摘要： Compositions for the diagnosis and therapy of prostate and colon cancer, derived from or based on a novel prostate-specific, androgen-regulated, cell membrane associated and secreted serine protease termed 20P1F12/TMPRSS2 are described. A full length cDNA comprising the entire coding sequence of the 20P1F12/TMPRSS2 gene (also designated 20P1F12-GTC1 herein) is provided (FIG. 1). Among the compositions provided are antibodies that bind to 20P1F12/TMPRSS2 proteins and polypeptide fragments thereof, including antibodies labeled with a detectable marker or toxin or therapeutic composition. The invention also provides prognostic and diagnostic methods of examining a biological sample for evidence of disregulated cellular growth by comparing the status of 20P1F12/TMPRSS2 in the biological sample to the status of 20P1F12/TMPRSS2 in a corresponding normal sample, wherein alterations in the status of 20P1F12/TMPRSS2 in the biological sample are associated with disregulated cellular growth. The invention further provides various therapeutic compositions and strategies for treating prostate cancer, including particularly, 20P1F12/TMPRSS2 polypeptide and anti-20P1F12/TMPRSS2 antibody therapy methods and compositions, cancer vaccines, and small molecule therapy.

摘要翻译： 描述了衍生自或基于称为20P1F12 / TMPRSS2的新型前列腺特异性，雄激素调节的细胞膜相关和分泌的丝氨酸蛋白酶的前列腺和结肠癌的诊断和治疗的组合物。 提供了包含20P1F12 / TMPRSS2基因（本文中也称为20P1F12-GTC1）的整个编码序列的全长cDNA（图1）。 所提供的组合物是结合20P1F12 / TMPRSS2蛋白和其多肽片段的抗体，包括用可检测标记或毒素或治疗组合物标记的抗体。 本发明还提供了通过将生物样品中20P1F12 / TMPRSS2的状态与对应的正常样品中的20P1F12 / TMPRSS2的状态进行比较来检查生物样品以检测细胞生长失调的证据的预后和诊断方法，其中， 生物样品中的20P1F12 / TMPRSS2与细胞生长失调有关。 本发明还提供了治疗前列腺癌的各种治疗组合物和策略，特别包括20P1F12 / TMPRSS2多肽和抗-20P1F12 / TMPRSS2抗体治疗方法和组合物，癌症疫苗和小分子治疗。

公开(公告)号：US07217799B2

公开(公告)日：2007-05-15

申请号：US09942052

申请日：2001-08-28

申请人： Arthur B. Raitano ,  Mary Faris ,  Rene S. Hubert ,  Daniel E. Afar ,  Wangmao Ge ,  Pia M. Challita-Eid ,  Aya Jakobovits

发明人： Arthur B. Raitano ,  Mary Faris ,  Rene S. Hubert ,  Daniel E. Afar ,  Wangmao Ge ,  Pia M. Challita-Eid ,  Aya Jakobovits

IPC分类号： C07K16/00 ,  C12P21/08 ,  A61K39/395 ,  C12P21/04 ,  C12N15/02 ,  C12N5/06 ,  C07H21/04

CPC分类号： C07K14/47

摘要： A novel gene (designated 85P1B3) and its encoded protein are described. While 85P1B3 exhibits tissue specific expression in normal adult tissue, it is aberrantly expressed in multiple cancers including set forth in Table 1. Consequently, 85P1B3 provides a diagnostic and/or therapeutic target for cancers. The 85P1B3 gene or fragment thereof, or its encoded protein or a fragment thereof, can be used to elicit an immune response.

摘要翻译： 描述了一种新型基因（命名为85P1B3）及其编码的蛋白质。 虽然85P1B3在正常成人组织中表现出组织特异性表达，但是在多种癌症中异常表达，如表1所示。因此，85P1B3为癌症提供了诊断和/或治疗靶点。 85P1B3基因或其片段或其编码的蛋白质或其片段可用于引发免疫应答。

公开(公告)号：US07351583B2

公开(公告)日：2008-04-01

申请号：US10991028

申请日：2004-11-16

申请人： Arthur B. Raitano ,  Daniel E. H. Afar ,  Aya Jakobovits ,  Mary Faris ,  Rene S. Hubert ,  Steven Chappell Mitchell ,  Douglas Saffran

发明人： Arthur B. Raitano ,  Daniel E. H. Afar ,  Aya Jakobovits ,  Mary Faris ,  Rene S. Hubert ,  Steven Chappell Mitchell ,  Douglas Saffran

IPC分类号： C12N15/85

CPC分类号： C07K16/28 ,  A01K2217/05 ,  A61K38/00 ,  A61K39/00 ,  C07K14/705 ,  C07K16/3069 ,  C07K2317/34

摘要： A novel gene (designated PHOR-1) that is highly over-expressed in prostate and other cancers and its encoded protein are described. PHOR-1 is a G protein-coupled receptor with homology to receptors involved in olfaction. PHOR-1 in normal human tissues is restricted to prostate, and this gene is highly over-expressed in prostate cancer as well as in cancers of the kidney, uterus, cervix, stomach and rectum. Consequently, PHOR-1 provides a diagnostic and/or therapeutic target for prostate cancer.

摘要翻译： 描述了在前列腺和其他癌症及其编码的蛋白质中高度过表达的新基因（称为PHOR-1）。 PHOR-1是与涉及嗅觉的受体具有同源性的G蛋白偶联受体。 正常人组织中的PHOR-1仅限于前列腺，并且该基因在前列腺癌以及肾，子宫，宫颈，胃和直肠的癌症中高度过度表达。 因此，PHOR-1为前列腺癌提供了诊断和/或治疗靶点。

公开(公告)号：US07208280B2

公开(公告)日：2007-04-24

申请号：US10001469

申请日：2001-10-31

申请人： Aya Jakobovits ,  Arthur B. Raitano ,  Daniel E. H. Afar ,  Douglas Saffran ,  Rene S. Hubert ,  Mary Faris ,  Pia M. Challita-Eid

发明人： Aya Jakobovits ,  Arthur B. Raitano ,  Daniel E. H. Afar ,  Douglas Saffran ,  Rene S. Hubert ,  Mary Faris ,  Pia M. Challita-Eid

IPC分类号： G01M33/53

CPC分类号： C07K14/47 ,  A01K2217/05 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/705 ,  C07K16/28 ,  C07K2317/34

摘要： A novel gene (designated 101P3A11 and also referred to as PHOR-1) and its encoded protein are described. While 101P3A11 exhibits tissue specific expression in normal adult tissue, it is aberrantly expressed in prostate, colon and kidney cancers. Thus, 101P3A11 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 101P3A11 gene or fragment thereof, or its encoded protein or a fragment thereof, can be used to elicit an immune response.

摘要翻译： 描述了一种新型基因（命名为101P3A11，也称为PHOR-1）及其编码的蛋白质。 虽然101P3A11在正常成人组织中表现出组织特异性表达，但在前列腺，结肠和肾癌中异常表达。 因此，101P3A11为癌症提供了诊断，预后，预防和/或治疗靶点。 101P3A11基因或其片段或其编码的蛋白质或其片段可用于引发免疫应答。