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    Sample well insert with wedge-shaped profile for ultra-thin slab gels in electrophoresis
    1.
    发明授权
    Sample well insert with wedge-shaped profile for ultra-thin slab gels in electrophoresis 失效
    样品孔插入物，用于电泳中超薄板状凝胶的楔形轮廓

    公开(公告)号：US5284565A

    公开(公告)日：1994-02-08

    申请号：US995495

    申请日：1992-12-23

    申请人： Daniel Y. Chu ,  Elaine R. Mardis

    发明人： Daniel Y. Chu ,  Elaine R. Mardis

    IPC分类号： B01D57/02 ,  C25B7/00 ,  G01N27/447 ,  G01N27/26

    CPC分类号： G01N27/44743

    摘要： A comb to be used for holding samples and defining sample lanes in an ultra-thin slab gel in vertical slab gel electrophoresis is constructed with to form tapered or wedged-shaped sample wells, with the narrowest edge of the wedge opening into the upper edge of the gel. In one embodiment, the comb has a wedge-shaped profile along the contact edge to provide an expanded well at each sample location. Notches alternating with grooves are cut into the wedge shape to form teeth which taper along orthogonal axes, the wells being defined by the space between each adjacent pair of teeth. The gel enclosure which holds the slab gel is formed by plates which are shaped along one edge to provide a complementary wedge-shaped opening leading into the gel space. The comb fits snugly into the opening with the teeth of the comb touching or extending a short distance into the upper exposed surface of the gel. In another embodiment, the comb has a rectangular profile, with grooves cut to a varying depth to form the wedge shape.

    摘要翻译： 用于在垂直板状凝胶电泳中用于保存样品并在超薄板状凝胶中定义样品通道的梳子被构造成形成锥形或楔形样品样品孔，其中楔形物的最窄边缘开口到 凝胶。 在一个实施例中，梳子具有沿着接触边缘的楔形轮廓，以在每个样品位置处提供扩展的孔。 与沟槽交替的凹槽被切割成楔形，以形成沿着正交轴线逐渐变细的齿，孔由每个相邻齿对之间的空间限定。 保持板状凝胶的凝胶封壳由沿着一个边缘成形的板形成，以提供通向凝胶空间的互补的楔形开口。 梳子紧密地贴合在开口中，梳子的牙齿接触或延伸到凝胶的上暴露表面的短距离。 在另一个实施例中，梳子具有矩形轮廓，其中凹槽切割成不同的深度以形成楔形。

    THERMAL CYCLER WITH VAPOR CHAMBER FOR RAPID TEMPERATURE CHANGES
    2.
    发明申请
    THERMAL CYCLER WITH VAPOR CHAMBER FOR RAPID TEMPERATURE CHANGES 有权
    具有蒸汽室的热循环器，用于快速温度变化

    公开(公告)号：US20130143272A1

    公开(公告)日：2013-06-06

    申请号：US13460983

    申请日：2012-05-01

    申请人： Kun Guo ,  Daniel Y. Chu ,  Paul Patt ,  Amir Sadri ,  Roger Tong

    发明人： Kun Guo ,  Daniel Y. Chu ,  Paul Patt ,  Amir Sadri ,  Roger Tong

    IPC分类号： B01L7/00

    CPC分类号： B01L7/52 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1855

    摘要： Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate.

    摘要翻译： 微孔板或包含反应孔或样品容器阵列的任何薄壁板的孔中的快速和均匀的温度变化通过使用加热和冷却元件实现，其中蒸气室插入在这些元件和微板之间。 在某些实施例中，蒸气室的上表面和样品板的下侧在形状上是互补的，即它们在每个样品容器周围的区域中具有相同但相反方向的轮廓，从而沿着 每个插座。 在其他实施例中，中间板被放置在蒸气室和井板之间，中间板的顶表面与孔板的下侧互补。

    COMPACT AUTOMATED CELL COUNTER
    3.
    发明申请
    COMPACT AUTOMATED CELL COUNTER 有权
    紧凑型自动电池柜台

    公开(公告)号：US20110211058A1

    公开(公告)日：2011-09-01

    申请号：US12869979

    申请日：2010-08-27

    申请人： Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

    发明人： Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

    IPC分类号： G06K9/00 ,  C12M1/00 ,  H04N7/18

    CPC分类号： C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

    摘要： Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

    摘要翻译： 液体悬浮液中的生物细胞在自动细胞计数器中计数，其将悬浮液的图像聚焦在包含至少4,000,000个像素的数字成像传感器上，每个具有2×2μm或更小的面积，并且图像的视场 至少3 mm2。 该传感器使得计数器能够将光学部件压缩成高度小于20cm的光路，当垂直布置时，整个光路的方向没有变化，并且整个仪器的占地面积小于300cm 2。 光源的激活，传感器图像的自动聚焦和数字单元计数都是通过将样品架简单插入到仪器中来启动的。 将悬浮液置于样品室中，其形状为成形以确保细胞计数器中载玻片的正确取向。

    Microplates with Ultra-Thin Walls by Two-Stage Forming
    5.
    发明申请
    Microplates with Ultra-Thin Walls by Two-Stage Forming 有权
    通过两阶段成型制成超薄壁微孔板

    公开(公告)号：US20100028988A1

    公开(公告)日：2010-02-04

    申请号：US12511839

    申请日：2009-07-29

    申请人： Daniel Y. Chu ,  Hui Du

    发明人： Daniel Y. Chu ,  Hui Du

    IPC分类号： C12M1/34 ,  B29C55/00

    CPC分类号： B29C51/04 ,  B01L3/50851 ,  B01L2200/12 ,  B01L2300/0829 ,  B29C51/02 ,  B29C2791/006 ,  B29K2023/06 ,  B29K2023/12

    摘要： A multi-well plate with wells having ultra-thin walls and yet with sufficient structural rigidity to allow reliable use of the plate in automated instrumentation is formed by first forming a plate blank by injection molding, the blank being of a thickness that provides the desired rigidity, and then subjecting the blank to vacuum forming to stretch designated areas in the blank to form wells or to extend wells already formed, the stretching resulting in a reduction in thickness of the molded resin at the walls of the wells only.

    摘要翻译： 具有超薄壁并且具有足够的结构刚性以允许在自动化仪器中可靠地使用板的多孔板通过首先通过注射成型形成板坯而形成，坯料具有提供期望的厚度 刚性，然后对坯料进行真空成形以拉伸坯料中的指定区域以形成孔或延伸已经形成的孔，拉伸仅导致在孔壁处的模制树脂的厚度减小。

    Electrophoresis slab gel enclosure for gradient gels
    6.
    发明授权
    Electrophoresis slab gel enclosure for gradient gels 失效
    梯度凝胶电泳板式凝胶封套

    公开(公告)号：US5626735A

    公开(公告)日：1997-05-06

    申请号：US249566

    申请日：1994-05-26

    申请人： Daniel Y. Chu

    发明人： Daniel Y. Chu

    IPC分类号： C25B9/00 ,  G01N27/447

    CPC分类号： G01N27/44704

    摘要： Gradient gels are formed in a slab gel enclosure by introducing gel solution through a channel in one of the two or more spacers which set the thickness of the gel. The channel opens into the gel space through an aperture in the inner longitudinal edge of the spacer at approximately mid-length along the edge, and filling of the gel space with the solution is performed with the enclosure rotated onto its side. In certain embodiments of the invention, the opposing spacer contains an aperture as well, plus a channel leading from the aperture to the exterior of the enclosure, for venting of air from the gel space as the space is being filled with the gel-forming liquid.

    摘要翻译： 通过将凝胶溶液通过设置凝胶厚度的两个或更多间隔物中的一个中的通道引入凝胶溶液形成梯度凝胶。 通道沿着边缘大致中等长度地通过间隔物的内纵向边缘中的孔通入凝胶空间，并且在外壳旋转到其侧面上时，用溶液填充凝胶空间。 在本发明的某些实施例中，相对的间隔物还包括孔，加上从孔到外壳的通道，用于当凝胶形成液体被填充空间时从凝胶空间排出空气 。

    Cam-operated gradient former
    7.
    发明授权
    Cam-operated gradient former 失效
    凸轮操纵梯度前

    公开(公告)号：US5540498A

    公开(公告)日：1996-07-30

    申请号：US282241

    申请日：1994-07-29

    申请人： Daniel Y. Chu

    发明人： Daniel Y. Chu

    IPC分类号： B01D57/02 ,  B01J4/02 ,  G01N27/447 ,  B01F15/02 ,  G05D11/02

    CPC分类号： G01N27/44704 ,  Y10T74/2107

    摘要： Gradient gels for electrophoresis are produced by an apparatus in which a pair of syringes are mounted on a support rack together with cams arranged for synchronous rotation. Cam followers engaged by the cams are linked to the syringe barrels, with each cam defining a distinct spiral path translated by one of the cam followers into a preselected rate or rate profile of volumetric discharge from one of the syringes as the cam rotates. With the rate or rate profile established by one cam being distinct from the rate or rate profile established by the other, a gradient or other variation in composition is obtained when the syringe discharges are combined into a common stream and directed to the gel enclosure for solidification into a gel. The gradient or variation in composition is determined by the cam profiles alone, and is independent of the rate at which the cams are rotated, and of whether or not the rotation is performed in a steady manner. The apparatus can be readily designed for manual operation with highly accurate and reproducible results.

    摘要翻译： 用于电泳的梯度凝胶通过其中一对注射器与配置为同步旋转的凸轮一起安装在支撑架上的装置来制造。 由凸轮啮合的凸轮从动件连接到注射器筒上，每个凸轮限定一个螺旋路径，其中一个凸轮从动件由凸轮从动件中的一个平移到预定速率或速度曲线，其中一个注射器随着凸轮旋转而进行。 由于一个凸轮确定的速率或速率曲线与另一个凸轮确定的速率或速率曲线不同，当将注射器排出物组合成共同的流并引导到凝胶外壳进行凝固时，获得组成的梯度或其他变化 变成凝胶。 组成的梯度或变化由单独的凸轮轮廓确定，并且与凸轮旋转的速率无关，并且是否以稳定的方式执行旋转。 该装置可以容易地设计用于具有高度精确和可重现的结果的手动操作。

    Microplates with ultra-thin walls by two-stage forming
    10.
    发明授权
    Microplates with ultra-thin walls by two-stage forming 有权
    具有超薄壁的微孔板通过两段成型

    公开(公告)号：US08802000B2

    公开(公告)日：2014-08-12

    申请号：US12511839

    申请日：2009-07-29

    申请人： Daniel Y. Chu ,  Hui Du

    发明人： Daniel Y. Chu ,  Hui Du

    IPC分类号： B29C51/10 ,  B29C51/04 ,  B01L3/00 ,  B29C51/02 ,  B29K23/00

    CPC分类号： B29C51/04 ,  B01L3/50851 ,  B01L2200/12 ,  B01L2300/0829 ,  B29C51/02 ,  B29C2791/006 ,  B29K2023/06 ,  B29K2023/12

    摘要： A multi-well plate with wells having ultra-thin walls and yet with sufficient structural rigidity to allow reliable use of the plate in automated instrumentation is formed by first forming a plate blank by injection molding, the blank being of a thickness that provides the desired rigidity, and then subjecting the blank to vacuum forming to stretch designated areas in the blank to form wells or to extend wells already formed, the stretching resulting in a reduction in thickness of the molded resin at the walls of the wells only.

    摘要翻译： 具有超薄壁并且具有足够的结构刚性以允许在自动化仪器中可靠地使用板的多孔板通过首先通过注射成型形成板坯而形成，坯料具有提供期望的厚度 刚性，然后对坯料进行真空成形以拉伸坯料中的指定区域以形成孔或延伸已经形成的孔，拉伸仅导致在孔壁处的模制树脂的厚度减小。