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公开(公告)号：US5310675A
公开(公告)日：1994-05-10
申请号：US805605
申请日：1991-12-10
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： Recombinant host bacteria and plasmids for making the bacteria using recombinant DNA techniques are described. The plasmids contain DNA coding for subtilisin with an amino acid substitution. Expression of the plasmid DNA results in production of a modified subtilisin.
摘要翻译： 描述了使用重组DNA技术制备细菌的重组宿主细菌和质粒。 质粒含有编码具有氨基酸取代的枯草杆菌蛋白酶的DNA。 质粒DNA的表达导致修饰的枯草杆菌蛋白酶的产生。
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公开(公告)号：US20090075332A1
公开(公告)日：2009-03-19
申请号：US12047157
申请日：2008-03-12
申请人： Eugenio Ferrari , David A. Estell
发明人： Eugenio Ferrari , David A. Estell
CPC分类号： C12N9/54 , C12Y304/21062
摘要： This invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the present invention relates to methods for altering the expression of proteases in microorganisms, such as Bacillus species. The invention discloses modified polynucleotides, vectors, modified polypeptides, and processes for enhancing the production of proteases.
摘要翻译： 本发明涉及编码修饰的蛋白酶的修饰多核苷酸，以及改变微生物中蛋白酶生成的方法。 特别地，本发明涉及用于改变微生物如芽孢杆菌属物种中蛋白酶表达的方法。 本发明公开了修饰的多核苷酸，载体，修饰的多肽以及用于增强蛋白酶生产的方法。
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公开(公告)号：US08779112B2
公开(公告)日：2014-07-15
申请号：US12047157
申请日：2008-03-12
申请人： Eugenio Ferrari , David A. Estell
发明人： Eugenio Ferrari , David A. Estell
CPC分类号： C12N9/54 , C12Y304/21062
摘要： This invention relates to modified polynucleotides encoding modified proteases, and methods for altering the production of proteases in microorganisms. In particular, the present invention relates to methods for altering the expression of proteases in microorganisms, such as Bacillus species. The invention discloses modified polynucleotides, vectors, modified polypeptides, and processes for enhancing the production of proteases.
摘要翻译： 本发明涉及编码修饰的蛋白酶的修饰多核苷酸，以及改变微生物中蛋白酶生成的方法。 特别地，本发明涉及用于改变微生物如芽孢杆菌属物种中蛋白酶表达的方法。 本发明公开了修饰的多核苷酸，载体，修饰的多肽以及用于增强蛋白酶生产的方法。
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4.发明授权Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors 失效非人羰基水解酶突变体，编码相同的载体和用所述载体转化的宿主
公开(公告)号：US5182204A
公开(公告)日：1993-01-26
申请号：US807786
申请日：1991-12-11
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54 , Y10S435/832
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to encode the substitution of an amino acid in the amino acid sequence of a precursor carbonyl hydrolase. Such mutants have properties which are different than the precursor hydrolase.
摘要翻译： 衍生自天然或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。 突变体羰基水解酶通常通过体外修饰编码天然存在或重组的羰基水解酶的前体DNA序列获得，以编码前体羰基水解酶的氨基酸序列中的氨基酸的取代。 这些突变体具有与前体水解酶不同的性质。
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公开(公告)号：US5346823A
公开(公告)日：1994-09-13
申请号：US36592
申请日：1993-03-24
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.
摘要翻译： 提供了制备具有改变的氧化稳定性的突变型枯草杆菌蛋白酶的方法，所述方法包括获得由编码枯草杆菌蛋白酶的区域组成的DNA片段，并将突变引入所述DNA片段，使得突变引入到编码 敏感的蛋氨酸，色氨酸，半胱氨酸或赖氨酸，使得突变型枯草杆菌蛋白酶表达后，编码甲硫氨酸，色氨酸，半胱氨酸或赖氨酸的一个或多个密码子区域被甲硫氨酸，色氨酸，半胱氨酸或赖氨酸以外的氨基酸替代， 优选丙氨酸或丝氨酸。
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公开(公告)号：USRE34606E
公开(公告)日：1994-05-10
申请号：US556918
申请日：1990-07-20
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： A cloned subtilsin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
摘要翻译： 已经在特定位点修饰了克隆的subtilisin基因，以在酶中的某些点处引起氨基酸取代。 优选由芽孢杆菌产生的修饰酶可与洗涤剂组合使用。
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公开(公告)号：US4760025A
公开(公告)日：1988-07-26
申请号：US614612
申请日：1984-05-29
申请人： David A. Estell , James A. Wells
发明人： David A. Estell , James A. Wells
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents.
摘要翻译： 克隆的枯草杆菌蛋白酶基因已在特定位点被修饰，以在酶中的某些位点引起氨基酸取代。 优选由芽孢杆菌产生的修饰酶可与洗涤剂组合使用。
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公开(公告)号：US5441882A
公开(公告)日：1995-08-15
申请号：US521010
申请日：1990-05-09
申请人： David A. Estell , James A. Wells , Richard R. Bott
发明人： David A. Estell , James A. Wells , Richard R. Bott
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： There are described methods for making mutant subtilisins, the methods comprising obtaining a DNA fragment from a Bacillus subtilisin and introducing a mutation into the fragment by substituting at least one amino acid, transforming a suitable host cell with the mutated DNA, recovering a mutant subtilisin and screening the mutant subtilisin for certain altered enzymatic properties.
摘要翻译： 描述了制备突变型枯草杆菌蛋白酶的方法，所述方法包括从枯草杆菌蛋白酶获得DNA片段，并通过用至少一个氨基酸取代突变进入该片段，用突变的DNA转化合适的宿主细胞，回收突变型枯草杆菌蛋白酶和 筛选突变的枯草杆菌蛋白酶以获得某些改变的酶特性。
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公开(公告)号：US20140154782A1
公开(公告)日：2014-06-05
申请号：US14115801
申请日：2012-05-04
申请人： Neelam S. Amin , Katherine Augustyn , Joshua R. Basler , Luis G. Cascao-Pereira , Katherine D. Collier , Edward M. Concar , David A. Estell , James T. Kellis, JR. , Euan John Magennis , Alexander Pisarchik , Ayrookaran J. Poulose , Philip Frank Souter , Glenn Steven Ward , Jian Yao
发明人： Neelam S. Amin , Katherine Augustyn , Joshua R. Basler , Luis G. Cascao-Pereira , Katherine D. Collier , Edward M. Concar , David A. Estell , James T. Kellis, JR. , Euan John Magennis , Alexander Pisarchik , Ayrookaran J. Poulose , Philip Frank Souter , Glenn Steven Ward , Jian Yao
摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.
摘要翻译： 本发明提供丝氨酸蛋白酶变体，更具体地是从其产生的枯草杆菌蛋白酶变体。 具体而言，与参考丝氨酸蛋白酶相比，本发明提供丝氨酸蛋白酶变体，更具体地具有一个或多个取代的枯草杆菌蛋白酶变体。 此外，本发明提供包含这些丝氨酸蛋白酶变体，更具体地枯草杆菌蛋白酶变体的组合物。 在一些实施方案中，本发明提供了包含这些丝氨酸蛋白酶变体，更具体地枯草杆菌蛋白酶变体中的至少一种的清洁组合物。
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公开(公告)号：US08394941B2
公开(公告)日：2013-03-12
申请号：US13082979
申请日：2011-04-08
申请人： Neelam S. Amin , Katherine D. Collier , Melodie Estabrook , David A. Estell , Bryan P. Fox , Scott D. Power , Brian F. Schmidt , Gudrun Vogtentanz
发明人： Neelam S. Amin , Katherine D. Collier , Melodie Estabrook , David A. Estell , Bryan P. Fox , Scott D. Power , Brian F. Schmidt , Gudrun Vogtentanz
IPC分类号： C12N15/00
CPC分类号： C07K14/811 , A61K8/64 , A61Q7/02
摘要： The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.
摘要翻译： 本发明涉及包含结合靶蛋白的肽的修饰变体Bowman Birk蛋白酶抑制剂蛋白（BBPI），并且被进一步修饰以具有比未修饰的BBPI更大的蛋白酶抑制活性和/或以更高的产量产生。 本发明包括多核苷酸构建体和含有编码修饰变体BBPI的多核苷酸序列的表达载体，表达和产生修饰变体BBPI的转化宿主细胞，修饰变体BBPI蛋白，包含修饰变体BBPI的组合物和制备方法 并在个人护理中使用修改后的变体BBPI。
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